Lactic Acid Bacteria: The Functions and Applications in Foods—2nd Edition

A special issue of Foods (ISSN 2304-8158). This special issue belongs to the section "Nutraceuticals, Functional Foods, and Novel Foods".

Deadline for manuscript submissions: 20 November 2026 | Viewed by 2373

Special Issue Editors


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Guest Editor
Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China
Interests: lactic acid bacteria; bioactive compounds; probiotics; prebiotics; postbiotics; gut microbiota; antibacterial; quorum sensing; biofilm; foodborne pathogens
Special Issues, Collections and Topics in MDPI journals

E-Mail Website
Guest Editor
College of Food Science, South China Agriculture University, Guangzhou 510642, China
Interests: lactic acid bacteria; probiotics; prebiotics; metabolism; metabolic syndrome; gut microbiota; biological activities
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The first edition of this Special Issue (https://www.mdpi.com/journal/foods/special_issues/5W0CHFT2X2) was a great success and gained the attention and interest of many scholars. Here, I take the opportunity to thank them for their contributions and support. As the topic continues to gain scholarly attention and play a pivotal role, we are pleased to announce that we are launching a second edition. We hope that it will be as successful as Volume I and greatly benefit the field.

Lactic acid bacteria (LAB), as natural microbiotas which can inhabit the human body, are generally recognized as safe, are widely applied in the food industry, and have a long history. Previous works have proven that LAB not only improve the texture and taste of food but also exhibit a variety of biological activities, such as antioxidant, hypoglycemic, antihypertensive, cholesterol-lowering, antibiofilm, and antibacterial. LAB synthesize many natural and health-promoting compounds, including organic acids, bacteriocins, aromatic compounds, fatty acids, and exopolysaccharides in the fermentation process. Additionally, the majority of LAB strains are used as probiotics, which exert many functions, such as regulating the gut microbiota and enhancing the immune system. Furthermore, LAB and their fermentation products can be used to prepare postbiotics, which are formulations of lifeless microorganisms and/or their components beneficial to host health.

Prof. Dr. Qingping Zhong
Prof. Dr. Zhenlin Liao
Guest Editors

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Keywords

  • lactic acid bacteria
  • bioactive compounds
  • probiotics
  • prebiotics
  • postbiotics
  • gut microbiota
  • antibacterial
  • quorum sensing
  • biofilm

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Published Papers (3 papers)

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Research

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12 pages, 2495 KB  
Article
Exopolysaccharides from Lactiplantibacillus plantarum WLPL04 Alleviate Hyperuricemia by Regulating Uric Acid Metabolism and Gut Microbiota
by Min Wei, Yingsheng Hu, Xiaoxian Li, Xingyi Long, Zhihong Zhang, Xueying Tao and Hua Wei
Foods 2026, 15(7), 1206; https://doi.org/10.3390/foods15071206 - 2 Apr 2026
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Abstract
Background: Hyperuricemia (HUA) is associated with excessive uric acid (UA) production, impaired renal excretion, and gut microbial dysbiosis. This study aims to systematically evaluate the alleviating effects of exopolysaccharide (EPS04) derived from Lactiplantibacillus plantarum WLPL04 on HUA. Methods: The study employed both in [...] Read more.
Background: Hyperuricemia (HUA) is associated with excessive uric acid (UA) production, impaired renal excretion, and gut microbial dysbiosis. This study aims to systematically evaluate the alleviating effects of exopolysaccharide (EPS04) derived from Lactiplantibacillus plantarum WLPL04 on HUA. Methods: The study employed both in vitro HK-2 cells and in vivo animal studies in HUA mice. Key methods included assessing xanthine oxidase (XOD) activity and reactive oxygen species (ROS) production in vitro and measuring serum UA levels, renal function parameters, XOD activity, and gene expression in vivo. Additionally, 16S rRNA sequencing was used to analyze gut microbiota composition. Results: EPS04 reduced UA production in HK-2 cells by inhibiting XOD activity and downregulating its gene expression while also decreasing XOD-derived ROS. EPS04 significantly lowered serum UA levels and attenuated renal injury in HUA mice. Hepatic XOD expression and activity were downregulated, reducing UA production, while UA excretion was enhanced through upregulation of renal ABCG2 expression. Furthermore, EPS04 increased gut microbiota α-diversity, restored the Bacteroidota/Firmicutes ratio, and enriched beneficial taxa, including Akkermansia and Dubosiella. Conclusions: EPS04 alleviates HUA through inhibition of XOD activity, upregulation of renal ABCG2 expression, and modulation of gut microbiota, suggesting its potential as a nutraceutical biopolymer for dietary management of HUA and related metabolic disorders. Full article
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21 pages, 1367 KB  
Article
Raw Milk Cheese Microbiomes: A Paradigm for Interactions of Lactic Acid Bacteria in Food Ecosystems
by Christine K. Olupot, Olivia Sheehan, Zoe Kampff, Brian McDonnell, David F. Woods, Gabriele Andrea Lugli, Marco Ventura, F. Jerry Reen, Douwe van Sinderen and Jennifer Mahony
Foods 2026, 15(7), 1160; https://doi.org/10.3390/foods15071160 - 30 Mar 2026
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Abstract
While industrial-scale dairy fermentations often employ pasteurized milk as the substrate, many farmhouse and traditional production practices apply raw milk derived from a variety of mammals. Certain artisanal production systems rely on the autochthonous microbiota of the milk, fermentation vessels, equipment and/or environment [...] Read more.
While industrial-scale dairy fermentations often employ pasteurized milk as the substrate, many farmhouse and traditional production practices apply raw milk derived from a variety of mammals. Certain artisanal production systems rely on the autochthonous microbiota of the milk, fermentation vessels, equipment and/or environment to initiate milk coagulation. While the technological properties of lactic acid bacteria associated with dairy fermentations are well described, their interactions with other organisms during fermentation and cheese ripening are poorly investigated. This study presents an overview of the microbial ecology of raw and pasteurized milk used in the production of Irish farmhouse cheeses using metagenomic and culture-based approaches. Metagenomic analysis of four raw milk-derived cheeses established the dominant presence of either lactococci or Streptococcus spp. and with a secondary population of various lactobacilli. Interestingly, the Brie sample was also demonstrated to possess significant proportion of Hafnia spp. This was corroborated in culture-based analysis where Hafnia isolates were also identified. Furthermore, we report on the motility phenotype, lactose utilization ability and metabolic products of isolates of Hafnia paralvei and Hafnia alvei, and determine that these strains could grow in a non-antagonistic manner on plates with strains of Lactococcus lactis and Streptococcus thermophilus. As artisanal and farmhouse production systems are often associated with protected or regionally significant products, it is essential to develop a clear understanding of the microbial communities within and the complex relationships between the community members. Full article
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Review

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21 pages, 1896 KB  
Review
Bioactive Peptides–Probiotics Interactions: Implications for Microbial Function and Human Health
by Yue Fan, Qingping Wu, Lanyan Huang, Ying Zhang, Xiuhua Lin, Qihui Gu and Moutong Chen
Foods 2026, 15(6), 979; https://doi.org/10.3390/foods15060979 - 10 Mar 2026
Cited by 1 | Viewed by 945
Abstract
Bioactive peptides (BPs) and probiotics have attracted increasing attention in food and nutrition research for their roles in microbial metabolism and functional food development, with lactic acid bacteria (LAB) representing widely used probiotic microorganisms possessing well-characterized metabolic and peptide transport systems within the [...] Read more.
Bioactive peptides (BPs) and probiotics have attracted increasing attention in food and nutrition research for their roles in microbial metabolism and functional food development, with lactic acid bacteria (LAB) representing widely used probiotic microorganisms possessing well-characterized metabolic and peptide transport systems within the gut microbiota. This review summarizes current knowledge on food-derived BPs and their interactions with probiotic LAB, with a particular focus on peptide transport and utilization mechanisms, including oligopeptide permease (Opp) and di-/tripeptide permease (Dpp) systems. Sources and production methods of BPs are reviewed, along with experimental evidence describing peptide-supported microbial growth and metabolic responses. Relevant analytical approaches used for peptide characterization and functional assessment are also discussed. Most available evidence derives from controlled in vitro studies and primarily reflects microbial physiological responses rather than direct host-level effects. This review provides a mechanistic perspective on peptide–probiotic interactions in LAB and outlines research directions related to nitrogen utilization and microbial functional performance. Full article
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