Listeria monocytogenes in the Food Chain: Detection, Control, Resistance, and Emerging Risks

A special issue of Foods (ISSN 2304-8158). This special issue belongs to the section "Food Microbiology".

Deadline for manuscript submissions: 20 July 2026 | Viewed by 1605

Editors


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Department of Food Science and Technology, Faculty of Veterinary, Agrifood Campus of International Excellence (ceiA3), University of Cordoba, 14014 Córdoba, Spain
Interests: food safety; food hygiene; predictive microbiology; microbial risk assessment; modeling; emerging technologies; biopreservation; antimicrobial resistance
Special Issues, Collections and Topics in MDPI journals

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Guest Editor
Department of Food Science and Technology, Centro Andaluz de Investigación de Zoonosis y Enfermedades Emergentes CAIZEM, Research Group HIBRO, UIC Zoonosis y Enfermedades Emergentes ENZOEM, University of Cordoba, 14014 Córdoba, Spain
Interests: microbial risk assessment; predicitive modeling; food safety; sustainable food packaging; preservation
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Listeria monocytogenes is a major foodborne pathogen of concern across the entire food chain, from primary production to consumption. Its ability to persist in food processing environments, resist environmental stressors, and grow under refrigeration makes it particularly challenging to control. The ongoing occurrence of listeriosis outbreaks, especially linked to ready-to-eat foods, highlights the need for continued monitoring.

This Special Issue invites original research and review articles focused on the detection, control, and characterization of L. monocytogenes throughout the food chain. Particular emphasis is placed on the emergence of antimicrobial-resistant and hypervirulent strains, biofilm formation, and the development of effective intervention strategies. Contributions addressing predictive microbiology, genomic tools, risk assessment, and innovative approaches to mitigate L. monocytogenes in diverse food matrices are especially welcome.

We look forward to your submissions.

Dr. Arícia Possas
Prof. Dr. Fernando Pérez-Rodríguez
Guest Editors

Manuscript Submission Information

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Keywords

  • ready-to-eat foods
  • antimicrobial restistance
  • persistence
  • biofilm
  • virulence
  • detection methods
  • predictive microbiology
  • risk assessment
  • genomic surveillance
  • listeriosis

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Published Papers (1 paper)

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Research

15 pages, 629 KB  
Article
Safety of Ready-to-Eat Green Leafy Salads: Growth Potential of Listeria monocytogenes During Shelf Life
by Muhammad-Ehtesham Abdul, Paolo Cipriani, Elena Cosciani-Cunico, Paola Monastero, Stefania Ducoli, Alessandro Norton, Daniela Merigo, Enrico Pavoni, Guido Finazzi, Marina-Nadia Losio and Elena Dalzini
Foods 2026, 15(7), 1136; https://doi.org/10.3390/foods15071136 - 25 Mar 2026
Viewed by 878
Abstract
Ready-to-eat (RTE) fresh salads are widely consumed for their convenience and nutritional value, but they could represent a relevant food safety concern, as they do not undergo a lethal heat treatment before consumption, and furthermore, they may support the growth of Listeria monocytogenes [...] Read more.
Ready-to-eat (RTE) fresh salads are widely consumed for their convenience and nutritional value, but they could represent a relevant food safety concern, as they do not undergo a lethal heat treatment before consumption, and furthermore, they may support the growth of Listeria monocytogenes during refrigerated storage. In this study, the growth potential of L. monocytogenes was evaluated by standardised challenge tests in five commercially available RTE salads: crispy lettuce, baby lettuce, a baby lettuce–spicy mustard mix, and two mâche products from different producers. Three different batches for each product were inoculated with a three-strain cocktail of L. monocytogenes at a target level of approximately 2–3 log CFU/g and stored under conditions simulating reasonably foreseeable refrigerated storage (7 °C for approximately two-thirds of their shelf life, followed by 10 °C for the remaining one-third), in accordance with ISO 20976-1 and EURL L. monocytogenes guidelines. The growth potential (Δ) was calculated as the difference between the highest mean L. monocytogenes concentration observed during storage and the mean of the initial concentration at time zero, both in three replicate samples; Δmax was defined as the highest Δ value among the tested batches. Crispy lettuce, baby lettuce, and the mixed salad supported the growth of L. monocytogenes, with Δmax values of 2.33, 2.60, and 3.65 log CFU/g, respectively. In contrast, both mâche products showed Δmax values ≤ 0.5 log CFU/g, indicating an inability to support pathogen growth under the tested conditions. These results demonstrate that the growth potential of L. monocytogenes in RTE salads is strongly product-specific and likely influenced by intrinsic characteristics and background microbiota, as well as by storage temperature. The findings underline the importance of strict temperature control and product-specific risk assessment to ensure compliance with microbiological criteria throughout shelf life and to mitigate the risk of listeriosis associated with RTE salads. Full article
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