Innovative Extraction Techniques of Bioactive Compounds from Plant-Based Foods: Characterization and Potential Applications

A special issue of Foods (ISSN 2304-8158). This special issue belongs to the section "Plant Foods".

Deadline for manuscript submissions: 31 October 2025 | Viewed by 4693

Special Issue Editors


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Guest Editor
Research Group for Bioactives–Analysis and Application, National Food Institute, Technical University of Denmark, Lyngby, Denmark
Interests: food science and technology; emerging technologies; natural products; bioactive compounds; green extraction; food processing; food agricultural wastes; chromatography

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Guest Editor
Grain Science and Industry Department, Kansas State University, Manhattan, KS 66506, USA
Interests: supercritical fluid technology; phenolic compounds; analytical chemistry; grain science; glycation; antioxidants; food processing; emerging technologies; agricultural wastes; bioactivity.
Special Issues, Collections and Topics in MDPI journals

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Guest Editor
Research Group for Bioactives–Analysis and Application, National Food Institute, Technical University of Denmark, Lyngby, Denmark
Interests: green chemistry; sustainable extraction technologies; waste valorization; bioactive compounds; modification of bioactive compounds
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

There is a growing interest in incorporating plant-based ingredients into food formulations, driven by sustainability concerns and consumer preferences. These ingredients include a wide variety of plant materials such as fruits, vegetables, cereals, legumes, herbs, and spices, among others. Consequently, advancing research and development in the extraction of bioactive compounds from plant foods has become a key focus in food science and biotechnology. Bioactive compounds—such as polyphenols, flavonoids, peptides, essential oils, and carotenoids—are of particular interest due to their antioxidant, anti-inflammatory, and anticancer properties, making them valuable as functional food ingredients.

Innovative methods, including ultrasound-assisted extraction, supercritical fluid extraction, pressurized liquid extraction, microwave-assisted extraction, and pulsed electric field-assisted extraction, have demonstrated significant potential in promoting the sustainable production of high-quality functional ingredients. This Special Issue aims to provide a platform for the dissemination of cutting-edge research on innovative techniques for the extraction and modification of bioactive compounds, with the goal of enhancing their bioactivity and functionality. We warmly invite researchers to contribute their latest findings to this Special Issue, fostering knowledge exchange and the development of novel solutions in the field of bioactive compounds extracted from plant foods.

Dr. Grazielle Náthia-Neves
Dr. Ádina L. Santana
Dr. Adane Tilahun Getachew
Guest Editors

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Keywords

  • green extraction methods
  • natural antioxidants
  • food biotechnology
  • phytochemicals
  • emerging technologies
  • clean label
  • functional ingredients
  • formulation of functional foods using bioactive extracts
  • process intensification using innovative technologies
  • bioavailability and bioaccessibility of natural bioactive compounds
  • biological activity of plant extracts
  • sensory evaluation of food products with plant extracts as ingredients

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Published Papers (2 papers)

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Research

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20 pages, 3945 KB  
Article
Ozone-Assisted Green Upgrading of Lactuca sativa Oil: Characterization and Bioactivity for Clean-Label Functional Applications
by Abdulrahman S. Bazaid, Sulaiman A. Alsalamah, Waleed Hakami, Mohammed Ibrahim Alghonaim, Amro Duhduh and Husam Qanash
Foods 2025, 14(20), 3458; https://doi.org/10.3390/foods14203458 - 10 Oct 2025
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Abstract
Ozonation is an emergent green technology that modifies the chemical composition and bioactivity of natural oils, creating new opportunities for functional and biomedical use. In this study, the chemical changes and in vitro activities of lettuce (Lactuca sativa) oil before and [...] Read more.
Ozonation is an emergent green technology that modifies the chemical composition and bioactivity of natural oils, creating new opportunities for functional and biomedical use. In this study, the chemical changes and in vitro activities of lettuce (Lactuca sativa) oil before and after ozonation were evaluated. Gas chromatography–mass spectrometry (GC–MS) revealed an increase in both the number and diversity of constituents in ozonated oil, with (Z)-13-docosenamide and trans-13-octadecenoic acid as predominant components. Fourier-transform infrared (FTIR) spectra showed overall similarity between native and ozonated oils, but with three additional characteristic bands in the ozonated sample. Bioassays demonstrated that ozonation enhanced anti-Helicobacter pylori activity (inhibition zone 21.3 ± 0.3 mm), supported bactericidal effects, and improved antibiofilm and antihemolytic properties. The antioxidant capacity of ozonated oil was modestly increased (IC50 = 3.95 ± 0.4 µg/mL), while butyrylcholinesterase inhibition was more markedly enhanced (IC50 = 2.58 ± 0.6 µg/mL), compared to that of the non-ozonated oil (IC50 = 6.14 ± 0.3 µg/mL and IC50 = 4.38 ± 0.4 µg/mL, respectively). Molecular docking suggested strong interactions of major ozonation-derived compounds with human BuChE and H. pylori urease, providing mechanistic support for the observed activities. Overall, these results indicate that ozonation modestly but consistently enhances the biological potential of lettuce oil through compositional shifts, highlighting its promise for development as a safe functional food ingredient with possible biomedical applications. Full article
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Review

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39 pages, 1456 KB  
Review
Legume Proteins in Food Products: Extraction Techniques, Functional Properties, and Current Challenges
by Grazielle Náthia-Neves, Adane Tilahun Getachew, Ádina L. Santana and Charlotte Jacobsen
Foods 2025, 14(9), 1626; https://doi.org/10.3390/foods14091626 - 4 May 2025
Cited by 2 | Viewed by 3671
Abstract
The aim of this review is to provide a comprehensive overview of protein extraction from legume sources, with a focus on both conventional and emerging techniques. Particular attention is given to the impact of innovative methods on protein functionality, a key factor for [...] Read more.
The aim of this review is to provide a comprehensive overview of protein extraction from legume sources, with a focus on both conventional and emerging techniques. Particular attention is given to the impact of innovative methods on protein functionality, a key factor for food applications. Due to their nutritional profile and techno-functional properties, legumes are increasingly regarded as promising alternatives to animal-based protein sources in the food industry. Traditional extraction methods, such as alkaline and acidic extraction, are discussed and compared with novel approaches including enzymatic extraction, ultrasound-assisted extraction (UAE), microwave-assisted extraction (MAE), ohmic heating (OH), subcritical water extraction (SWE), deep eutectic solvents (DES), and dry fractionation. The potential of these emerging technologies to improve protein yield and functionality is critically assessed, alongside key challenges such as scalability, cost-effectiveness, and potential allergenicity. This review also identifies current research gaps and highlights opportunities for innovation in sustainable protein extraction. Therefore, this review contributes to the development of more efficient, functional, and sustainable protein ingredients production, highlighting the role of innovative extraction technologies in shaping the future of plant-based foods. Full article
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