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Recent Advances in Biofuel Production from Microalgae Biomass

A special issue of Energies (ISSN 1996-1073). This special issue belongs to the section "A4: Bio-Energy".

Deadline for manuscript submissions: 30 May 2025 | Viewed by 5505

Special Issue Editor


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Guest Editor
Department of Renewable Energy Engineering, West Pomeranian University of Technology, Pawla VI 1, 71-459 Szczecin, Poland
Interests: microalgal biomass; biogas production; biofuels; bioenergy; waste management; environmental engineering; microbial fuel cells; bioremediation

Special Issue Information

Dear Colleagues,

The use of fossil fuels for energy production leads to increased pollution levels, including the amount of greenhouse gases emitted, and has global economic consequences. An alternative way to cover the increasing energy demand is to use renewable sources, including biomass. However, the production of bioenergy must be sustainable and environmentally friendly, not competing with food production, both in terms of land (low ILUC) and the components necessary for cultivation, i.e., water and nutrients. Conventional biomass use, the method of obtaining such feedstocks, and their availability raise concerns; therefore, a great deal of attention is focused on algal biomass. The distinguishing properties of microalgae are their growth rate, their potential for the fixation of carbon dioxide during photosynthesis, and their ability to accumulate valuable biocomponents. Microalgal biomass is a kind of green magazine where solar energy has been stored in the form of various chemical compounds. An appropriate conversion method makes it possible to produce heat, electricity, or liquid and gaseous biofuels, including bioethanol, biodiesel, green diesel, biomethane, biohydrogen, syngas, and bio-oil, from the biomass or components present in the microalgae cells. Microalgal biomass is not only a renewable energy substrate but also a biocatalyst for hydrogen production via biophotolysis and electrofermentation in microbial electrolysis cells. Biofuels from microalgae have the potential to promote energy diversification in the future and, due to the high level of carbon dioxide sequestration in biomass, to decarbonize transport. Biofuel production can be combined with bioremediation ('phycoremediation') processes favouring the microalgae-mediated removal of different environmental pollutants with lower cultivation costs. The commercialization of these technologies requires intensified research for efficient and low-cost biomass production conducted on a larger scale, optimization of environmental and technical cultivation conditions, efficient and low-cost harvesting, and refined conversion methods. There is a need for economic analyses of microalgal biofuel production to indicate the current level of competitiveness compared to other conventional energy carriers. Sustainable production of microalgal biomass, integrated energy conversion technologies in biorefineries, and appropriate regulations are key areas for the development of commercial microalgal biofuel production.

Prof. Małgorzata Hawrot-Paw
Guest Editor

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Keywords

  • microalgae
  • microalgal biomass culture
  • microalgal biomass conversion
  • advanced biofuel
  • bioethanol
  • biodiesel
  • syngas
  • biohydrogen
  • biomethane
  • bioenergy
  • biophotovoltaic (BPV)
  • biorefinery
  • biomass management
  • energy efficiency
  • microbial electrolysis cells (MECs)
  • energy storage
  • anaerobic digestion
  • transport decarbonization
  • biophotolysis

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Published Papers (4 papers)

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Research

14 pages, 2289 KiB  
Article
Characterization of Lipid Production in Chlorella sp. Cultivated in Different Plant Fertilizers
by Bipasha Chakrabarty, Łukasz Barczak, Paulina Rusanowska, Magda Dudek and Marcin Zieliński
Energies 2024, 17(23), 6193; https://doi.org/10.3390/en17236193 - 9 Dec 2024
Cited by 2 | Viewed by 877
Abstract
Microalgae have gained attention due to their higher reproduction rate and lipid productivity. In particular, various stress conditions lead to an overproduction of lipids in microalgae cells. The study investigated the influence of additional CO2 introduced with air into the reactor during [...] Read more.
Microalgae have gained attention due to their higher reproduction rate and lipid productivity. In particular, various stress conditions lead to an overproduction of lipids in microalgae cells. The study investigated the influence of additional CO2 introduced with air into the reactor during biomass growth of Chlorella sp. Additionally, increased phosphorus concentration in the medium under stress cultivation (low nitrogen concentration) was examined. The partial pressure of CO2 and its increased availability to Chlorella sp. in the cultivation medium increased biomass growth (1.4 times) and chlorophyll content (2.5 times) in microalgae cells. A high phosphorus fertilizer significantly increased lipid production under stress conditions with CO2 supply to 85.2 mg/g (2.6 times) and without CO2 to 73.8 mg/g (2.2 times). A high concentration of phosphorus in the culture medium stimulated the synthesis of C16:0 (about 38–45%) and C18:1 CIS9 (about 24–30%). The results confirm that the fertilizers can be used as a culture medium to induce stress and stimulate lipid production. Adjusting the composition of the fertilizers and controlling the additional CO2 supply could prove beneficial to increase the content of the desired fatty acids. Full article
(This article belongs to the Special Issue Recent Advances in Biofuel Production from Microalgae Biomass)
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15 pages, 2606 KiB  
Article
Comparison of Biohydrogen Production by Tetraselmis subcordiformis During Cultivation Using Soil-Less Agricultural Wastewater and Effluent from Microbial Fuel Cells
by Marcin Zieliński, Łukasz Barczak, Paulina Rusanowska, Joanna Kazimierowicz and Marcin Dębowski
Energies 2024, 17(21), 5287; https://doi.org/10.3390/en17215287 - 24 Oct 2024
Cited by 2 | Viewed by 1147
Abstract
The development and implementation of innovative production technologies have a direct influence on the creation of new sources of pollution and types of waste. An example of this is the wastewater from soil-less agriculture and the effluent from microbial fuel cells. An important [...] Read more.
The development and implementation of innovative production technologies have a direct influence on the creation of new sources of pollution and types of waste. An example of this is the wastewater from soil-less agriculture and the effluent from microbial fuel cells. An important topic is the development and application of methods for their neutralisation that take into account the assumptions of global environmental policy. The aim of the present study was to determine the possibilities of utilising this type of pollution in the process of autotrophic cultivation of the biohydrogen-producing microalgae Tetraselmis subcordiformis. The highest biomass concentration of 3030 ± 183 mgVS/L and 67.9 ± 3.5 mg chl-a/L was observed when the culture medium was wastewater from soil-less agriculture. The growth rate in the logarithmic growth phase was 270 ± 16 mgVS/L-day and 5.95 ± 0.24 mg chl-a/L-day. In the same scenario, the highest total H2 production of 161 ± 8 mL was also achieved, with an observed H2 production rate of 4.67 ± 0.23 mL/h. Significantly lower effects in terms of biomass production of T. subcordiformis and H2 yield were observed when fermented dairy wastewater from the anode chamber of the microbial fuel cell was added to the culture medium. Full article
(This article belongs to the Special Issue Recent Advances in Biofuel Production from Microalgae Biomass)
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20 pages, 1173 KiB  
Article
Analysis of Multi-Biofuel Production during Cultivation of the Green Microalga Tetraselmis subscordiformis
by Marcin Dębowski, Magda Dudek, Joanna Kazimierowicz, Piera Quattrocelli, Paulina Rusanowska, Łukasz Barczak, Anna Nowicka and Marcin Zieliński
Energies 2024, 17(15), 3670; https://doi.org/10.3390/en17153670 - 25 Jul 2024
Cited by 2 | Viewed by 909
Abstract
Research to date has mainly focused on the properties and efficiency of the production of selected, individual types of biofuels from microalgae biomass. There are not enough studies investigating the efficiency of the production of all energy sources synthesised by these microorganisms in [...] Read more.
Research to date has mainly focused on the properties and efficiency of the production of selected, individual types of biofuels from microalgae biomass. There are not enough studies investigating the efficiency of the production of all energy sources synthesised by these microorganisms in a single technological cycle. The aim of this research was to determine the possibilities and efficiency of the production of hydrogen, bio-oil, and methane in the continuous cycle of processing T. subcordiformis microalgae biomass. This study showed it was feasible to produce these three energy carriers, but the production protocol adopted was not necessarily valuable from the energy gain standpoint. The production of bio-oil was found to be the least viable process, as bio-oil energy value was only 1.3 kWh/MgTS. The most valuable single process for microalgae biomass conversion turned out to be methane fermentation. The highest specific gross energy gain was found after applying a protocol combining biomass production, hydrogen biosynthesis, and subsequent methane production from T. subcordiformis biomass, which yielded a total value of 1891.4 kWh/MgTS. The direct methane fermentation of T. subcordiformis biomass enabled energy production at 1769.8 kWh/MgTS. Full article
(This article belongs to the Special Issue Recent Advances in Biofuel Production from Microalgae Biomass)
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12 pages, 1536 KiB  
Article
Efficient Production of Microalgal Biomass—Step by Step to Industrial Scale
by Małgorzata Hawrot-Paw and Patryk Ratomski
Energies 2024, 17(4), 944; https://doi.org/10.3390/en17040944 - 18 Feb 2024
Viewed by 1647
Abstract
The production of microalgal biomass on a commercial scale remains a significant challenge. Despite the positive results obtained in the laboratory, there are difficulties in obtaining similar results in industrial photobioreactors. Changing the cultivation conditions can affect not only the growth of microalgae [...] Read more.
The production of microalgal biomass on a commercial scale remains a significant challenge. Despite the positive results obtained in the laboratory, there are difficulties in obtaining similar results in industrial photobioreactors. Changing the cultivation conditions can affect not only the growth of microalgae but also their metabolism. This is of particular importance for the use of biomass for bioenergy production, including biofuel production. The aim of this study was to determine the biomass production efficiency of selected microalgal strains, depending on the capacity of the photobioreactor. The lipid and ash content of the biomass were also taken into account. It was found that as the scale of production increased, the amount of biomass decreased, irrespective of the type of strain. The change in scale also affected the lipid content of the biomass. The highest values were found in 2.5 L photobioreactors (ranging from 26.3 ± 2.2% for Monoraphidium to 13.9 ± 0.3% for Chlorella vulgaris). The least favourable conditions were found with industrial photobioreactors, where the lipid content of the microalgal biomass ranged from 7.1 ± 0.6% for Oocycstis submarina to 10.2 ± 1.2% for Chlorella fusca. The increase in photobioreactor capacity had a negative effect on the ash content. Full article
(This article belongs to the Special Issue Recent Advances in Biofuel Production from Microalgae Biomass)
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