Topical Collection "Diagnostic Virology"

Editor

Prof. Dr. Nuno Taveira
Website
Collection Editor
Egas Moniz University Institute, Almada, Portugal; Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, Lisbon, Portugal
Interests: virus epidemiology; transmission dynamics; diversity and drug resistance; production/validation of new diagnostic tests; development of new antiviral drugs; development of new HIV vaccine and microbicide candidates

Topical Collection Information

Dear Colleagues,

In these troubled COVID-19 times we are living in, it has become more evident than ever that rapid, deployable, and highly sensitive, specific and precise diagnostic assays can save many lives. Together with the established qPCR-based and isothermal amplification-based methods, new technologies such as those based on CRISPR-Cas13 system and metagenomics are emerging and are enabling the rapid and sensitive detection and identification of multiple viruses at the same time with an unprecedented quality, both in the lab and outside the lab (PoC assays). These molecular methods of diagnostics rely on nucleic acid extraction methods whose performance characteristics may be very diverse and need a better assessement. High quality serologic assays are also paramount for the diagnosis and management of virus diseases, and for monitoring the response to vaccination. A better understanding of the advantages and limitations of currently available molecular and serologic assays for the diagnosis, treatment management, and cure assessement of each virus disease is needed. There is no better time for this Special Issue in diagnostic virology. I would like to invite you to submit your best review or original work on all aspects of diagnostic virology mentioned above and others that I have missed to this topical collection issue. We are particularly seeking top level contributions on the development, validation, and implementation of molecular and serologic assays for the diagnosis, prognosis, and management of diseases caused by human viruses in all settings from the lab to the house and the bed of the patient.  

Prof. Dr. Nuno Taveira
Collection Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the collection website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Diagnostics is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Detecton and identification of viral pathogens
  • Development and validation of diagnostic tests (molecular, antigenic and serologic)
  • Implementation of diagnostic tests in different settings and geographies
  • Performance of nucleic acid extraction tests
  • Point of care assays
  • Multiplex assays
  • CRISPR diagnostics
  • Metagenomic diagnostics
  • Drug resistance assays
  • Diagnostic virology and public health

Published Papers (1 paper)

2020

Open AccessArticle
Accuracy of the Zika IgM Antibody Capture Enzyme-Linked Immunosorbent Assay from the Centers for Disease Control and Prevention (CDC Zika MAC-ELISA) for Diagnosis of Zika Virus Infection
Diagnostics 2020, 10(10), 835; https://doi.org/10.3390/diagnostics10100835 - 18 Oct 2020
Abstract
Serological diagnosis of Zika virus (ZIKV) infection is challenging because of antigenic cross-reactivity with dengue virus (DENV). This study evaluated the accuracy of the Zika IgM antibody capture enzyme-linked immunosorbent assay (CDC Zika IgM MAC-ELISA) in differentiating between ZIKV and DENV infections. To [...] Read more.
Serological diagnosis of Zika virus (ZIKV) infection is challenging because of antigenic cross-reactivity with dengue virus (DENV). This study evaluated the accuracy of the Zika IgM antibody capture enzyme-linked immunosorbent assay (CDC Zika IgM MAC-ELISA) in differentiating between ZIKV and DENV infections. To determine sensitivity, we used acute- and convalescent-phase sera from 21 patients with RT-PCR-confirmed ZIKV infection. To determine specificity, we used acute- and convalescent-phase sera from 60 RT-PCR-confirmed dengue cases and sera from 23 blood donors. During the acute-phase of the illness, the assay presented a sensitivity of 12.5% (2/16) for samples collected 0–4 days post symptoms onset (DPSO), and of 75.0% (3/4) for samples collected 5–9 DPSO. During the convalescent-phase of the illness, the test sensitivity was 90.9% (10/11), 100% (2/2), and 0% (0/2) for samples obtained 12–102, 258–260, and 722–727 DPSO, respectively. Specificity for acute- and convalescent-phase samples from RT-PCR-confirmed dengue cases was 100% and 93.2%, respectively. Specificity for blood donor samples was 100%. The assay is an accurate method for Zika serological diagnosis and proved to be reliable for use during surveillance and outbreak investigations in settings where ZIKV and DENV cocirculate. Full article
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Graphical abstract

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