Laboratory Diagnosis in Microbial Diseases, 3rd Edition

A special issue of Diagnostics (ISSN 2075-4418). This special issue belongs to the section "Diagnostic Microbiology and Infectious Disease".

Deadline for manuscript submissions: 30 June 2025 | Viewed by 707

Special Issue Editor


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Guest Editor
Department of Laboratory Medicine, Chang Gung Memorial Hospital at Linkou, Taoyuan City 33305, Taiwan
Interests: laboratory medicine; medical AI; translational medicine
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Special Issue Information

Dear Colleagues,

Current diagnosis of infectious diseases largely depends on conventional phenotypic tools. However, typically, phenotypic tools are heavily based on culture and susceptible to bias due to handling during testing. Specifically, the phenotypic result represents the final phenomenon of protein expression in vitro. The details of genomics and proteomics cannot be seen in conventional phenotypic assays. What we can currently see is just the tip of the iceberg of infectious diseases. Thus, I would like to invite outstanding researchers in this field to contribute studies to this Special Issue. Areas of interest include, but are not limited to, genomic or proteomic studies focused on broadening our view on infectious diseases. I believe that these will form a fundamental basis for, and have a considerable impact on, further precise measurements of infectious diseases.

Dr. Hsin-Yao Wang
Guest Editor

Manuscript Submission Information

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Keywords

  • genomics
  • proteomics
  • diagnostics
  • infections

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Published Papers (1 paper)

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Research

13 pages, 2900 KiB  
Article
Comparative Performance Evaluation of Continuous Monitoring Blood Culture Systems Using Simulated Septic Specimen
by Kwangjin Ahn, Taesic Lee, Sangwon Hwang, Dong Min Seo and Young Uh
Diagnostics 2025, 15(4), 468; https://doi.org/10.3390/diagnostics15040468 - 14 Feb 2025
Viewed by 539
Abstract
Background/Objectives: Continuous monitoring blood culture systems (CMBCSs) are revolutionary automated instruments that facilitate the rapid identification of pathogens in blood samples from patients with sepsis. However, with only a few CMBCSs being widely used as references, user dependency on these limited options has [...] Read more.
Background/Objectives: Continuous monitoring blood culture systems (CMBCSs) are revolutionary automated instruments that facilitate the rapid identification of pathogens in blood samples from patients with sepsis. However, with only a few CMBCSs being widely used as references, user dependency on these limited options has grown. In response, a new CMBCS was developed and compared with existing systems to evaluate microbial growth. Methods: HubCentra84 was compared to BacT/Alert® 3D and BACTEC™ FX. Staphylococcus aureus, Streptococcus pneumoniae, Escherichia coli, Pseudomonas aeruginosa, Bacteroides fragilis, and Candida albicans were selected as representative clinically infectious microorganisms. Colonies from pure cultures were diluted with 0.9% saline to create simulated sepsis specimens (SSSs). The SSSs were injected into dedicated culture bottles for each instrument. Thirty paired tests were performed for each strain. Results: Colony-forming units of the added SSSs were consistent according to bacteria, and all strains demonstrated robust growth in three CMBCSs. Time-to-positivity was uniformly observed according to the instruments used. The novel CMBCS detected the growth of the clinically significant bacteria S. aureus, S. pneumoniae, E. coli, and P. aeruginosa approximately 2 h faster than the other two systems. However, it was approximately 200 min slower for C. albicans and 3000 min for B. fragilis. Conclusions: The novel CMBCS demonstrates advantages in detecting the growth of common clinical bacteria. Although slow growth was detected for certain microorganisms, it successfully captured the growth of all tested microorganisms. Full article
(This article belongs to the Special Issue Laboratory Diagnosis in Microbial Diseases, 3rd Edition)
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