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5.1
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Cells
Special Issues
Advances in Cell-Based Studies and Therapeutic Approaches for Tissue Regeneration
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Advances in Cell-Based Studies and Therapeutic Approaches for Tissue Regeneration

A special issue of Cells (ISSN 2073-4409). This special issue belongs to the section "Tissues and Organs".

Deadline for manuscript submissions: 31 October 2025 | Viewed by 1981

Special Issue Editors

Prof. Dr. Anişoara Cîmpean

E-Mail Website
Guest Editor
Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Bucharest, 91-95 Splaiul Independentei, 050095 Bucharest, Romania
Interests: biomedical materials; cell biomaterial interactions; biocompatibility; tissue engineering; regenerative medicine
Special Issues, Collections and Topics in MDPI journals
Dr. Andreea-Mariana Negrescu

E-Mail Website
Guest Editor Assistant
Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Bucharest, 91-95 Splaiul Independentei, 050095 Bucharest, Romania
Interests: biomaterials; biocompatibility; tissue engineering; regenerative medicine; cell biology; biomedical materials
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Regenerative medicine is an exciting multidisciplinary field of research that has gradually matured over the last two decades, offering the promise of advanced therapy and the restoration of an impaired or lost function, by regenerating, maintaining, repairing, or, in some severe cases, replacing the disease-compromised tissue or organ. Thus, the novel therapeutic approaches resulting from regenerative medicine and tissue engineering technology may offer new hope for patients with injuries, end-stage organ failure, or other clinical issues. Since the field of regenerative medicine involves the combination of various disciplines, such as medicine, molecular and cellular biology, materials science, and bioengineering, in this Special Issue we encourage the submission of manuscripts that provide new insights on topics related not only to novel stem cell therapies, but also on the use of a wide range of biomaterials and biotechnologies for soft and hard tissue regeneration, with a particular focus on the degenerative and inflammatory diseases. Original research articles and reviews are both welcomed. We look forward to your contributions.

Prof. Dr. Anişoara Cîmpean
Dr. Andreea Mariana Negrescu
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Cells is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • soft/hard tissue regeneration
  • therapeutical approaches
  • cell culture studies
  • inflammation-based diseases
  • degenerative diseases

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Further information on MDPI's Special Issue policies can be found here.

Published Papers (3 papers)

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Research

19 pages, 9842 KiB  
Article
Heat Preconditioning of Nanofat Does Not Improve Its Vascularization Properties
by Francesca Bonomi, Ettore Limido, Andrea Weinzierl, Caroline Bickelmann, Emmanuel Ampofo, Yves Harder, Michael D. Menger and Matthias W. Laschke
Cells 2025, 14(8), 581; https://doi.org/10.3390/cells14080581 - 11 Apr 2025
Viewed by 186
Abstract
Heat preconditioning has been shown to promote nutritive perfusion and tissue survival in autologous fat grafting as well as in flap and breast surgery. However, its impact on the vascularization properties of nanofat has not been investigated so far. Therefore, we exposed nanofat [...] Read more.
Heat preconditioning has been shown to promote nutritive perfusion and tissue survival in autologous fat grafting as well as in flap and breast surgery. However, its impact on the vascularization properties of nanofat has not been investigated so far. Therefore, we exposed nanofat from donor mice to a temperature of 43 °C for 1 h and assessed the effects of this heat stress on cell viability and the expression of heat shock proteins (HSPs) and angiogenesis-related factors. Moreover, dermal substitutes seeded with heat-preconditioned and non-preconditioned control nanofat were implanted into dorsal skinfold chambers of recipient mice to study their vascularization and tissue integration in vivo by means of repeated intravital fluorescence microscopy, histology and immunohistochemistry. Heat preconditioning upregulated the expression of HSPs in nanofat without affecting cell viability. Moreover, it resulted in the downregulation of many pro-angiogenic factors and the increased expression of anti-angiogenic factors, indicating a shift towards an anti-angiogenic phenotype. Accordingly, implanted dermal substitutes seeded with heat-preconditioned nanofat exhibited a reduced vascularization and were not better integrated into the host tissue when compared to controls. These findings indicate that heat preconditioning cannot be recommended for enhancing the vascularization capacity of nanofat. Full article
(This article belongs to the Special Issue Advances in Cell-Based Studies and Therapeutic Approaches for Tissue Regeneration)
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19 pages, 3962 KiB  
Article
Platelet-Rich Plasma Extract Derived from Animals Shows Potential in Promoting Wound Healing and Suppressing Inflammatory Response in Skin Cells
by Zheng-Qi Wang, Queenie Wing-Sze Lai, Xiong Gao, Qi-Yun Wu, Tina Ting-Xia Dong and Karl Wah-Keung Tsim
Cells 2025, 14(7), 526; https://doi.org/10.3390/cells14070526 - 1 Apr 2025
Viewed by 377
Abstract
Platelet-rich plasma (PRP) is an extract enriched with growth factors that facilitate skin regeneration and rejuvenation. Here, the functionalities of PRP derived from various animal sources have been investigated and compared, focusing on its potential therapeutic applications in skin regeneration. Total antioxidant capacity, [...] Read more.
Platelet-rich plasma (PRP) is an extract enriched with growth factors that facilitate skin regeneration and rejuvenation. Here, the functionalities of PRP derived from various animal sources have been investigated and compared, focusing on its potential therapeutic applications in skin regeneration. Total antioxidant capacity, wound closure, and melanin content in cultured keratinocytes were used to evaluate the efficacy of different animal PRP sources. The PRP derived from deer exhibited the highest performance and was selected for subsequent proteomic and metabolomic analyses. Our findings indicate that deer blood is an optimal source of animal-derived PRP, demonstrating significant properties in promoting wound healing, anti-inflammatory responses, and skin regeneration. This identified PRP from deer sources can be developed as a safe and effective product for skin rejuvenation and regeneration. Full article
(This article belongs to the Special Issue Advances in Cell-Based Studies and Therapeutic Approaches for Tissue Regeneration)
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25 pages, 13761 KiB  
Article
The Potential of a Novel Cold Atmospheric Plasma Jet as a Feasible Therapeutic Strategy for Gingivitis—A Cell-Based Study
by Andreea-Mariana Negrescu, Leonardo Zampieri, Emilio Martines and Anisoara Cimpean
Cells 2024, 13(23), 1970; https://doi.org/10.3390/cells13231970 - 28 Nov 2024
Viewed by 915
Abstract
Due to its antimicrobial, anti-inflammatory and pro-healing properties, the application of cold atmospheric plasma (CAP) has emerged as a new and promising therapeutic strategy in various fields of medicine, including general medicine and dentistry. In this light, the aim of the present study [...] Read more.
Due to its antimicrobial, anti-inflammatory and pro-healing properties, the application of cold atmospheric plasma (CAP) has emerged as a new and promising therapeutic strategy in various fields of medicine, including general medicine and dentistry. In this light, the aim of the present study was to investigate the effects of a homemade plasma jet on the cellular behaviour of two important cell types involved in gingivitis, namely gingival fibroblasts (HGF-1 cell line) and macrophages (RAW 264.7 cell line), by the direct application of CAP in different experimental conditions. The cellular behaviour of the HGF-1 cells was investigated in terms of viability/proliferation (LIVE/DEAD and CCK-8 assays), morphological features (immunofluorescent staining of the actin cytoskeleton) and fibronectin expression (immunocytochemical staining of the fibronectin network), while the macrophages’ response was evaluated through the assessment of the cellular survival/proliferation rate (LIVE/DEAD and CCK-8 assays), morphological behaviour (immunofluorescent staining of the actin cytoskeleton) and inflammatory activity (pro-inflammatory cytokine secretion profile (ELISA assay) and foreign body giant cells (FBGCs) formation (immunofluorescent staining of the actin cytoskeleton and multinuclearity index determination)). The in vitro biological assessment revealed an upward trend dependent on treatment time and number of CAP applications, in terms of fibroblasts proliferation (p < 0.0001) and fibronectin expression (p < 0.0001). On the other hand, the macrophages exposed to five consecutive CAP applications for longer treatment times (over 120 s) exhibited a strong pro-inflammatory activity, as evinced by their altered morphology, pro-inflammatory cytokine profile (p < 0.0001) and FBGCs formation. Overall, our results demonstrate that CAP exposure, when used with appropriate operating parameters, has a beneficial effect on the cellular response of HGF-1 and RAW 264.7 cells, thus paving the way for further in vitro and in vivo investigations that will allow the translation of CAP treatment from research to clinic as an alternative therapy for gingivitis. Full article
(This article belongs to the Special Issue Advances in Cell-Based Studies and Therapeutic Approaches for Tissue Regeneration)
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