Synthesis, Characterization and Applications of Collagen-Based Gels

A special issue of Gels (ISSN 2310-2861). This special issue belongs to the section "Gel Processing and Engineering".

Deadline for manuscript submissions: 30 September 2025 | Viewed by 567

Special Issue Editors


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Guest Editor
Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Bucharest, 91-95 Splaiul Independentei, 050095 Bucharest, Romania
Interests: biomaterials; biocompatibility; tissue engineering; regenerative medicine; cell biology; biomedical materials

Special Issue Information

Dear Colleagues,

Due to their distinctive characteristics, namely their biocompatibility, accessibility, affordability, and programmable properties, biopolymers are employed in a multitude of fields, including pharmaceutical and biomedical research, as well as cosmetics and food. Being the most abundant protein in mammals, with a triple-helix structure and an innately biocompatible, biomimetic, biodegradable, and haemostatic nature, collagen became one of the most commonly used biopolymers. Moreover, owing to the remarkable capacity of its fibers to self-assemble via non-covalent bonds and form in situ gels, collagen has been exploited in a plethora of applications, such tissue engineering, drug delivery systems, skin care, etc. The advantage of collagen-based gels is that they can be readily manipulated, and their mechanical and physical properties can be enhanced by chemical cross-linkers. However, sometimes, collagen-based gels face some limitations in the form of a weak mechanical strength and lack of elasticity. In light of these considerations, the field of collagen-based gels represents an ongoing area of research that requires a more comprehensive understanding of the technologies involved in their synthesis, gel formulations, and emerging applications. Thus, with this Special Issue, we aim to gather researchers who are interested in the field of collagen-based gels, from both a theoretical and applicative perspective. In particular, we welcome submissions presenting new insights into their fabrication, optimization parameters, drug loading and release mechanisms, the formulation and manufacturing aspects of novel collagen-based gels, and their innovative applications, with an emphasis on their biomedical use.

Dr. Andreea-Mariana Negrescu
Prof. Dr. Anişoara Cîmpean
Guest Editors

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Keywords

  • collagen-based gels
  • soft materials
  • mechanical properties
  • functional applications
  • tissue engineering
  • regenerative medicine
  • drug delivery

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Published Papers (1 paper)

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Research

23 pages, 6412 KiB  
Article
Influence of Cell Seeding Density and Material Stiffness on Chondrogenesis of Human Stem Cells Within Soft Hydrogels, Without the Use of Exogenous Growth Factors
by Arianna De Mori, Nadide Aydin, Giada Lostia, Alessia Manca, Gordon Blunn and Marta Roldo
Gels 2025, 11(3), 213; https://doi.org/10.3390/gels11030213 - 18 Mar 2025
Viewed by 417
Abstract
Mesenchymal stem cells (MSCs) can differentiate into chondrocytes provided with the appropriate environmental cues. In this study, we loaded human adipose-derived stem cells (hAdMSCs) into collagen/alginate hydrogels, which have been shown to induce chondrogenesis in ovine bone marrow stem cells without the use [...] Read more.
Mesenchymal stem cells (MSCs) can differentiate into chondrocytes provided with the appropriate environmental cues. In this study, we loaded human adipose-derived stem cells (hAdMSCs) into collagen/alginate hydrogels, which have been shown to induce chondrogenesis in ovine bone marrow stem cells without the use of any exogenous chondrogenic growth factors. We examined the influence of hydrogel stiffness (5.75 and 6.85 kPa) and cell seeding density (1, 2, 4, and 16 × 106 cells/mL) on the chondrogenic induction of hAdMSCs, without exogenous differentiation growth factors. Over time, the behaviour of the hAdMSCs in the scaffolds was investigated by analysing the amount of DNA; their morphology; their cell viability; the expression of chondrogenic genes (RT-qPCR); and the deposition of collagen I, collagen II, and aggrecan. The results showed that all scaffolds supported the acquisition of a rounded morphology and the formation of cell aggregates, which were larger with higher cell seeding densities. Furthermore, the cells were viable within the hydrogels throughout the experiment, indicating that high cell density did not have a detrimental effect on viability. All the conditions supported the upregulation of chondrogenic genes (SOX9, COL2A1, SOX5, and ACAN). By comparison, only the highest cell seeding density (16 × 106 cells/mL) promoted a superior extracellular matrix deposition composed of collagen II and aggrecan with limited production of collagen I. These molecules were deposited in the pericellular space. Furthermore, no histological difference was noted between the two stiffnesses. Full article
(This article belongs to the Special Issue Synthesis, Characterization and Applications of Collagen-Based Gels)
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