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Biosensors
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Advanced Nanozyme for Biosensors
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Advanced Nanozyme for Biosensors

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensors and Healthcare".

Deadline for manuscript submissions: closed (28 February 2025) | Viewed by 6160

Special Issue Editor

Prof. Dr. Zhen Zhang

E-Mail Website
Guest Editor
School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China
Interests: monoclonal antibody and nano-antibody of new pollutants; rapid analysis; immunoassays; biosensors and electrochemistry; signal amplification; cell imaging based on DNA functional materials
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Natural enzymes with remarkable efficiency and good substrate specificity can catalyze biochemical reactions in various biological processes, but enzymes are hampered in biosensing applications due to their fragility under terrible conditions. Fortunately, the emergence of nanozymes with enzyme-like activity provides a possibility for exploring the candidates of natural enzymes. Nowadays, diverse nanozymes based on oxidase, peroxidase, superoxide dismutase, and laccase have been widely developed, which exhibit advantages such as a low cost, high stability, high activity, and ease of storage, showing enormous potential in biosensing applications. Therefore, the present Special Issue mainly focuses on the advanced nanozyme for biosensors, particularly in the areas of the fabrication of novel nanozymes, the strategy of enhancing the nanozyme activity, and their biosensing applications in environment monitoring, food safety, medical diagnosis, etc. We sincerely invite authors to submit manuscripts to further promote the basic study of nanozymes and expand their applications in various fields.

Prof. Dr. Zhen Zhang
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2200 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • nanozyme
  • biosensing
  • environment monitoring
  • food safety
  • medical diagnosis

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Published Papers (4 papers)

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Research

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13 pages, 4938 KiB  
Article
Spermine Enhances the Peroxidase Activities of Multimeric Antiparallel G-quadruplex DNAzymes
by Raphael I. Adeoye, Theresia K. Ralebitso-Senior, Amanda Boddis, Amanda J. Reid, Francesca Giuntini, Amos A. Fatokun, Andrew K. Powell, Adaoha Ihekwaba-Ndibe, Sylvia O. Malomo and Femi J. Olorunniji
Biosensors 2025, 15(1), 12; https://doi.org/10.3390/bios15010012 - 2 Jan 2025
Viewed by 874
Abstract
G-quadruplex (G4) DNAzymes with peroxidase activities hold potential for applications in biosensing. While these nanozymes are easy to assemble, they are not as efficient as natural peroxidase enzymes. Several approaches are being used to better understand the structural basis of their reaction mechanisms, [...] Read more.
G-quadruplex (G4) DNAzymes with peroxidase activities hold potential for applications in biosensing. While these nanozymes are easy to assemble, they are not as efficient as natural peroxidase enzymes. Several approaches are being used to better understand the structural basis of their reaction mechanisms, with a view to designing constructs with improved catalytic activities. Spermine alters the structures and enhances the activities of some G4 DNAzymes. The reported effect of spermine in shifting the conformation of some G4 DNAzymes from antiparallel to parallel has not been tested on multimeric G4 DNAzymes. In this study, we examined the effects of spermine on the catalytic activities of multivalent constructs of Bcl2, c-MYC, PS2.M, and PS5.M. Our findings show that spermine significantly improved the peroxidase activity of PS2.M, an antiparallel G4 DNAzyme, while there was no significant effect on c-MYC, which already exists in a parallel conformation. The addition of spermine led to a substantial increase in the initial velocity of PS2.M and its multimeric form, enhancing it by approximately twofold. Therefore, spermine enhancement offers promise in expanding the range of DNAzymes available for use as biosensing tools. Full article
(This article belongs to the Special Issue Advanced Nanozyme for Biosensors)
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13 pages, 4866 KiB  
Article
Construction of Metal–Organic Framework as a Novel Platform for Ratiometric Determination of Cyanide
by Zongbao Sun, Zhiwei Wu, Yiran Zong, Chen Li, Wang Guo, Yiqing Guo and Xiaobo Zou
Biosensors 2024, 14(6), 276; https://doi.org/10.3390/bios14060276 - 27 May 2024
Cited by 1 | Viewed by 1242
Abstract
Metal–organic frameworks (MOFs) are frequently utilized as sensing materials. Unfortunately, the low conductivity of MOFs hinder their further application in electrochemical determination. To overcome this limitation, a novel modification strategy for MOFs was proposed, establishing an electrochemical determination method for cyanides in Baijiu. [...] Read more.
Metal–organic frameworks (MOFs) are frequently utilized as sensing materials. Unfortunately, the low conductivity of MOFs hinder their further application in electrochemical determination. To overcome this limitation, a novel modification strategy for MOFs was proposed, establishing an electrochemical determination method for cyanides in Baijiu. Co and Ni were synergistically used as the metal active centers, with meso−Tetra(4−carboxyphenyl)porphine (TCPP) and Ferrocenecarboxylic acid (Fc−COOH) serving as the main ligands, synthesizing Ni/Co−MOF−TCPP−Fc through a hydrothermal method. The prepared MOF exhibited improved conductivity and stable ratio signals, enabling rapid and sensitive determination of cyanides. The screen−printed carbon electrodes (SPCE) were suitable for in situ and real−time determination of cyanide by electrochemical sensors due to their portability, low cost, and ease of mass production. A logarithmic linear response in the range of 0.196~44 ng/mL was demonstrated by this method, and the limit of detection (LOD) was 0.052 ng/mL. Compared with other methods, the sensor was constructed by a one−step synthesis method, which greatly simplifies the analysis process, and the determination time required was only 4 min. During natural cyanide determinations, recommended readouts match well with GC−MS with less than 5.9% relative error. Moreover, this electrochemical sensor presented a promising method for assessing the safety of cyanides in Baijiu. Full article
(This article belongs to the Special Issue Advanced Nanozyme for Biosensors)
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11 pages, 7827 KiB  
Communication
Morphology Control of Zr-Based Luminescent Metal-Organic Frameworks for Aflatoxin B1 Detection
by Fang Zhu, Qiuxue Chai, Dinghui Xiong, Nuanfei Zhu, Jialong Zhou, Ruoxi Wu and Zhen Zhang
Biosensors 2024, 14(6), 273; https://doi.org/10.3390/bios14060273 - 27 May 2024
Cited by 3 | Viewed by 1227
Abstract
Metal–organic frameworks (MOFs) have gained significant prominence as sensing materials owing to their unique properties. However, understanding the correlation between the morphology, properties, and sensing performance in these MOF-based sensors remains a challenge, limiting their applications and potential for improvement. In this study, [...] Read more.
Metal–organic frameworks (MOFs) have gained significant prominence as sensing materials owing to their unique properties. However, understanding the correlation between the morphology, properties, and sensing performance in these MOF-based sensors remains a challenge, limiting their applications and potential for improvement. In this study, Zr-MOF was chosen as an ideal model to explore the impact of the MOF morphology on the sensing performance, given its remarkable stability and structural variability. Three luminescent MOFs (namely rod-like Zr-LMOF, prismoid-like Zr-LMOF, and ellipsoid-like Zr-LMOF) were synthesized by adjusting the quantities of the benzoic acid and the reaction time. More importantly, the sensing performance of these Zr-LMOFs in response to aflatoxin B1 (AFB1) was thoroughly examined. Notably, the ellipsoid-like Zr-LMOF exhibited significantly higher sensitivity compared to other Zr-LMOFs, attributed to its large specific surface area and pore volume. Additionally, an in-depth investigation into the detection mechanism of AFB1 by Zr-LMOFs was conducted. Building upon these insights, a ratiometric fluorescence sensor was developed by coordinating Eu3+ with ellipsoid-like Zr-LMOF, achieving a remarkably lower detection limit of 2.82 nM for AFB1. This study contributes to an improved comprehension of the relationship between the MOF morphology and the sensing characteristics while presenting an effective approach for AFB1 detection. Full article
(This article belongs to the Special Issue Advanced Nanozyme for Biosensors)
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Review

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22 pages, 3998 KiB  
Review
Mitigating Antibiotic Resistance: The Utilization of CRISPR Technology in Detection
by Xuejiao Zhang, Zhaojie Huang, Yanxia Zhang, Wen Wang, Zihong Ye, Pei Liang, Kai Sun, Wencheng Kang, Qiao Tang and Xiaoping Yu
Biosensors 2024, 14(12), 633; https://doi.org/10.3390/bios14120633 - 20 Dec 2024
Viewed by 2222
Abstract
Antibiotics, celebrated as some of the most significant pharmaceutical breakthroughs in medical history, are capable of eliminating or inhibiting bacterial growth, offering a primary defense against a wide array of bacterial infections. However, the rise in antimicrobial resistance (AMR), driven by the widespread [...] Read more.
Antibiotics, celebrated as some of the most significant pharmaceutical breakthroughs in medical history, are capable of eliminating or inhibiting bacterial growth, offering a primary defense against a wide array of bacterial infections. However, the rise in antimicrobial resistance (AMR), driven by the widespread use of antibiotics, has evolved into a widespread and ominous threat to global public health. Thus, the creation of efficient methods for detecting resistance genes and antibiotics is imperative for ensuring food safety and safeguarding human health. The clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (Cas) systems, initially recognized as an adaptive immune defense mechanism in bacteria and archaea, have unveiled their profound potential in sensor detection, transcending their notable gene-editing applications. CRISPR/Cas technology employs Cas enzymes and guides RNA to selectively target and cleave specific DNA or RNA sequences. This review offers an extensive examination of CRISPR/Cas systems, highlighting their unique attributes and applications in antibiotic detection. It outlines the current utilization and progress of the CRISPR/Cas toolkit for identifying both nucleic acid (resistance genes) and non-nucleic acid (antibiotic micromolecules) targets within the field of antibiotic detection. In addition, it examines the current challenges, such as sensitivity and specificity, and future opportunities, including the development of point-of-care diagnostics, providing strategic insights to facilitate the curbing and oversight of antibiotic-resistance proliferation. Full article
(This article belongs to the Special Issue Advanced Nanozyme for Biosensors)
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