Recent Advances in Fluorescent Probes: From Structure Design to Biological Applications

A special issue of Biomolecules (ISSN 2218-273X). This special issue belongs to the section "Chemical Biology".

Deadline for manuscript submissions: 31 December 2025 | Viewed by 1115

Special Issue Editor


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Guest Editor
Department of Bioengineering, University of California, Berkeley, CA 94720, USA
Interests: fluorescent probes; lipid nanoparticles for mRNA delivery; polymer

Special Issue Information

Dear Colleagues,

Fluorescent probes have become indispensable in studying biological processes due to their capacity for real-time visualization and quantification of molecular events. These probes are highly versatile, with applications in detecting and monitoring various biomolecules, such as ions, metabolites, proteins, and nucleic acids. The activity or expression of these biomolecules often shifts in response to various human diseases, including cancer, neurodegenerative disorders, cardiovascular diseases, and infectious diseases. Such changes can disrupt cellular functions and contribute to disease onset and progression. As a result, organic small molecule fluorescent probes are increasingly utilized as diagnostic tools, facilitating early disease detection, disease progression monitoring, and treatment efficacy assessment. The ongoing development of probes with improved sensitivity, specificity, and biocompatibility continues to propel advancements in the field. These innovations enhance our understanding of disease mechanisms and open new avenues for the personalized management of numerous health conditions, making these probes vital for modern biomedical research and clinical applications. In this Special Issue, we welcome articles and reviews on research advances in Fluorescent Probes.

Dr. Yalin Qi
Guest Editor

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Keywords

  • fluorescent probes
  • organic small molecule fluorescent probes
  • drug discovery
  • disease detection

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Published Papers (1 paper)

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Research

12 pages, 1538 KiB  
Article
Application of the Chitooligosaccharides and Fluorescence Polarization Technique for the Assay of Active Lysozyme in Hen Egg White
by Liliya I. Mukhametova, Dmitry O. Zherdev, Sergei A. Eremin, Pavel A. Levashov, Hans-Christian Siebert, Yury E. Tsvetkov, Olga N. Yudina, Vadim B. Krylov and Nikolay E. Nifantiev
Biomolecules 2024, 14(12), 1589; https://doi.org/10.3390/biom14121589 - 12 Dec 2024
Cited by 1 | Viewed by 863
Abstract
This study describes the applicability of the fluorescence polarization assay (FPA) based on the use of FITC-labeled oligosaccharide tracers of defined structure for the measurement of active lysozyme in hen egg white. Depending on the oligosaccharide chain length of the tracer, this method [...] Read more.
This study describes the applicability of the fluorescence polarization assay (FPA) based on the use of FITC-labeled oligosaccharide tracers of defined structure for the measurement of active lysozyme in hen egg white. Depending on the oligosaccharide chain length of the tracer, this method detects both the formation of the enzyme-to-tracer complex (because of lectin-like, i.e., carbohydrate-binding action of lysozyme) and tracer splitting (because of chitinase activity of lysozyme). Evaluation of the fluorescence polarization dynamics enables simultaneous measurement of the chitinase and lectin activities of lysozyme, which is crucial for its detection in complex biological systems. Hen egg white lysozyme (HEWL), unlike human lysozyme (HL), formed a stable complex with the chitotriose tracer that underwent no further transformations. This fact allows for easy measurement of the carbohydrate-binding activity of the HEWL. The results of the lysozyme activity measurement for hen egg samples obtained through the FPA correlated with the results obtained using the traditional turbidimetry method. The FPA does not have the drawbacks of turbidimetry, which are associated with the need to use bacterial cells that cannot be precisely standardized. Additionally, FPA offers advantages such as rapid analysis, the use of compact equipment, and standardized reagents. Therefore, the new express technique for measuring the lysozyme activity is applicable for evaluating the complex biomaterial, including for the purposes of food product quality control. Full article
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