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Journal of Fungi, Volume 9, Issue 7
July 2023 - 89 articles
Cover Story: Fungal mycotoxins have impacted the human food chain for thousands of years. The glycosylation of mycotoxins is a detoxification mechanism employed by plants, which is now also observed in selected fungal species. This includes the biocontrol agent Clonostachys rosea that converts 15-acetyl-DON (15-ADON) into 15-ADON-3-O-glycoside (15-ADON-3G). To identify the enzyme(s) responsible for this action, a protein structural comparison strategy targeting uridine diphosphate-glycosyl transferases (UDP-GTs) was applied. Selected targets were functionally characterized by CRISPR/Cas9 knockout, revealing multiple UDP-GTs, each with partial glycosylation activity against 15-ADON. This expands our understanding of natural mycotoxin detoxification, with potential applications to food security. View this paper
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J. Fungi - ISSN 2309-608X