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Journal of Fungi, Volume 8, Issue 6
June 2022 - 99 articles
Cover Story: Creating unique microenvironments, hyphal surfaces allow for spatially distinct functions and fungal bacterial interactions at the microscale. Using microfluidic technology, we attached >2400 single-cell pH bioreporters (Synechocystis sp. PCC6803) to the immediate hyphal surface of growing Coprinopsis cinerea hyphae to resolve the local pH at the microscale. The pH along hyphal surfaces was acidic. It varied significantly with pH at hyphal tips, being on the average 0.8 pH units lower than at more mature hyphal parts. These data represent the first dynamic in vitro analysis of surface pH along growing hyphae at the micrometer and improve our understanding of spatial, pH-dependent hyphal processes, such as the degradation of organic matter. View this paper
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J. Fungi - ISSN 2309-608X