Veterinary Sciences

21 pages, 681 KiB

Open AccessArticle

Fine Mapping Identifies Candidate Genes Associated with Swine Inflammation and Necrosis Syndrome

by Katharina Gerhards, Sabrina Becker, Josef Kühling, Joel Mickan, Mirjam Lechner, Hermann Willems and Gerald Reiner

Vet. Sci. 2025, 12(5), 508; https://doi.org/10.3390/vetsci12050508 - 21 May 2025

Viewed by 459

Abstract

Swine inflammation and necrosis syndrome (SINS) is a widespread disease in pigs, causing pain, suffering, and damage. Inflammation is documented at different levels based on clinical signs, histopathology, clinical chemistry, metabolomics and transcriptomics. The influence of sow and boar, as well as a [...] Read more.

Swine inflammation and necrosis syndrome (SINS) is a widespread disease in pigs, causing pain, suffering, and damage. Inflammation is documented at different levels based on clinical signs, histopathology, clinical chemistry, metabolomics and transcriptomics. The influence of sow and boar, as well as a heritability of around 0.3, suggest a genetic component to the disease. The aim of the present study was to identify functional single nucleotide polymorphisms (SNPs) in the vicinity of gene markers previously mapped using GWAS. DNA samples were available from 234 already phenotyped piglets. These animals were re-sequenced with additional prior enrichment. The nine selected chromosomal regions cover a total length of 22 Mbp. The genome-wide association study (GWAS) revealed two series with a total of 15 significant missense polymorphisms on chromosomes 11, 14, and 15. The homozygous genotypes of the most discriminating SNPs in series 1 resulted in SINS scores of 3.5 and 17.9, respectively. Despite the partial linkage of the SNPs, interesting candidate genes were defined. The results allow a significant narrowing of the possible candidate genes for understanding the pathogenesis of SINS and for future use in selection breeding to overcome the syndrome. Further studies should be carried out on larger animal populations. Full article

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23 pages, 659 KiB

Open AccessArticle

Polyhexamethylene Biguanide Nanoparticles Inhibit Biofilm Formation by Mastitis-Causing Staphylococcus aureus

by Renata de Freitas Leite, Breno Luis Nery Garcia, Kristian da Silva Barbosa, Thatiane Mendes Mitsunaga, Carlos Eduardo Fidelis, Bruna Juliana Moreira Dias, Renata Rank de Miranda, Valtencir Zucolotto, Liam Good and Marcos Veiga dos Santos

Vet. Sci. 2025, 12(5), 507; https://doi.org/10.3390/vetsci12050507 - 21 May 2025

Viewed by 807

Abstract

Staphylococcus aureus is a mastitis pathogen that compromises cow health and causes significant economic losses in the dairy industry. High antimicrobial resistance and biofilm formation by S. aureus limit the efficacy of conventional treatments. This study evaluated the potential of polyhexamethylene biguanide nanoparticles [...] Read more.

Staphylococcus aureus is a mastitis pathogen that compromises cow health and causes significant economic losses in the dairy industry. High antimicrobial resistance and biofilm formation by S. aureus limit the efficacy of conventional treatments. This study evaluated the potential of polyhexamethylene biguanide nanoparticles (PHMB NPs) against mastitis-causing S. aureus. PHMB NPs showed low toxicity to bovine mammary epithelial cells (MAC-T cells) at concentrations up to four times higher than the minimum inhibitory concentration (1 µg/mL) against S. aureus. In Experiment 1, PHMB NPs significantly reduced biofilm formation by S. aureus by 50% at concentrations ≥1 µg/mL, though they showed limited efficacy against preformed biofilms. In Experiment 2, using an excised teat model, PHMB NPs reduced S. aureus concentrations by 37.57% compared to conventional disinfectants (chlorhexidine gluconate, povidone–iodine, and sodium dichloroisocyanurate), though limited by short contact time. These findings highlight the potential of PHMB NPs for the control of S. aureus growth and biofilm formation. Full article

(This article belongs to the Special Issue Advancements in Livestock Staphylococcus sp.)

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17 pages, 4541 KiB

Open AccessArticle

Environment-Associated Variations in Blood Metabolism of Mongolian Cattle Grazing in the Alxa Desert of China

by Chao Hai, Dongchao Pei, Yuqing Yang, Lishuang Song, Xuefei Liu, Chunling Bai, Guanghua Su, Lei Yang and Guangpeng Li

Vet. Sci. 2025, 12(5), 506; https://doi.org/10.3390/vetsci12050506 - 21 May 2025

Viewed by 420

Abstract

Desert environments pose severe challenges to livestock survival. This study examined climate-driven physiological and metabolic adaptations in 258 Mongolian cattle from six regions of the Alxa Desert, China. Serum biochemical indices were measured and analyzed using linear models to assess the effects of [...] Read more.

Desert environments pose severe challenges to livestock survival. This study examined climate-driven physiological and metabolic adaptations in 258 Mongolian cattle from six regions of the Alxa Desert, China. Serum biochemical indices were measured and analyzed using linear models to assess the effects of climate, sex, and age. Climate significantly affected key blood parameters, including glucose (p < 0.001), creatinine (p < 0.001), alkaline phosphatase (p < 0.001), and lactate (p = 0.034). Additionally, sex significantly influenced lactate dehydrogenase (p = 0.049), bicarbonate (p = 0.0061), urea (p = 0.0055), and triglycerides (p = 0.039), while age affected total protein (p = 0.020), LDL-C (p = 0.0097), and cholesterol (p < 0.001). Glucose levels were negatively correlated with body size traits. Metabolomic profiling showed that cattle in arid, high-radiation areas exhibited reduced TCA cycle and fatty acid metabolism, with concurrent carbohydrate accumulation, including glucose, fructose, and mannose. Enhanced amino acid metabolism increased proline, valine, tyrosine, and tryptophan levels, potentially supporting physiological stability under heat and drought stress. These findings reveal how Mongolian cattle modulate metabolism in response to desert climates, offering insights into livestock adaptation and informing breeding strategies for resilience in harsh environments. Full article

(This article belongs to the Section Nutritional and Metabolic Diseases in Veterinary Medicine)

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27 pages, 654 KiB

Open AccessReview

Bluetongue’s New Frontier—Are Dogs at Risk?

by Rita Payan-Carreira and Margarida Simões

Vet. Sci. 2025, 12(5), 505; https://doi.org/10.3390/vetsci12050505 - 20 May 2025

Viewed by 913

Abstract

Bluetongue virus (BTV), traditionally considered a pathogen of ruminants, has recently been documented in dogs, challenging conventional understanding of its epidemiology. This narrative review synthesizes emerging evidence regarding BTV infections in domestic and wild carnivores, examining transmission dynamics, pathogenesis, clinical manifestations, and diagnostic [...] Read more.

Bluetongue virus (BTV), traditionally considered a pathogen of ruminants, has recently been documented in dogs, challenging conventional understanding of its epidemiology. This narrative review synthesizes emerging evidence regarding BTV infections in domestic and wild carnivores, examining transmission dynamics, pathogenesis, clinical manifestations, and diagnostic challenges. Carnivores can become infected through vector transmission and oral ingestion of infected material. While some infected carnivores remain subclinical, others develop severe clinical manifestations including hemorrhagic syndromes. BTV infection in carnivores is likely underdiagnosed due to limited awareness, nonspecific clinical signs, and absence of established diagnostic protocols for non-ruminant species. The potential role of carnivores in BTV epidemiology remains largely unexplored, raising questions about their function as reservoirs or dead-end hosts. Additionally, carnivores may contribute to alternative transmission pathways and overwintering mechanisms that impact disease ecology. Current biosecurity frameworks and surveillance systems, primarily focused on ruminants, require expansion to incorporate carnivores in viral maintenance and transmission. This review identifies significant knowledge gaps regarding BTV in carnivores and proposes future research directions, including serological surveys, transmission studies, and investigation of viral tropism in carnivore tissues. A comprehensive One Health approach integrating diverse host species, vector ecology, human interference, and environmental factors is crucial for effective BTV control and impact mitigation on human, animals, and environment. Full article

(This article belongs to the Section Veterinary Biomedical Sciences)

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17 pages, 2691 KiB

Open AccessArticle

Epidemiological Exploration of Cryptosporidium spp., Giardia intestinalis, Enterocytozoon bieneusi, and Blastocystis spp. in Yaks: Investigating Ecological and Zoonotic Dynamics in Lhasa, Xizang

by Yaru Ji, Munwar Ali, Chang Xu, Jia Wang, Md. F. Kulyar, Shah Nawaz, Khalid Mehmood, Mingming Liu and Kun Li

Vet. Sci. 2025, 12(5), 504; https://doi.org/10.3390/vetsci12050504 - 20 May 2025

Viewed by 372

Abstract

The yak (Bos grunniens), prevalent at an altitude between 3000 and 5000 m above sea level, provides the local inhabitants with meat, milk, leather, fuel (dung), and transport. However, intestinal zoonotic parasites seriously endanger its holistic well-being. The prime concern of [...] Read more.

The yak (Bos grunniens), prevalent at an altitude between 3000 and 5000 m above sea level, provides the local inhabitants with meat, milk, leather, fuel (dung), and transport. However, intestinal zoonotic parasites seriously endanger its holistic well-being. The prime concern of this study is to investigate the prevalence of four globally ubiquitous zoonotic enteric protozoans, namely Cryptosporidium spp., G. intestinalis, Blastocystis spp., and E. bieneusi in yaks from different areas of Lhasa, Xizang. In the given study, 377 yak fecal samples from various regions in Lhasa were obtained, including 161 samples from Linzhou County, 66 samples from Dangxiong County, and 150 samples from the Nimu County cattle farms. Molecular identification of these protozoans was done after amplification using PCR and sequencing of PCR-positive samples, and further phylogenetic analysis was performed. The results indicated that the prevalence of Cryptosporidium spp., G. intestinalis, E. bieneusi, and Blastocystis spp. in yak farms in Linzhou County was 48.5, 22.9, 47.8, and 90.7%; 65.2, 13.6, 72.7, and 87.9% in Dangxiong County; and 56.0, 29.3, 58.0, and 80.0%, respectively, in Nimu County. The results of this study provide a basic reference for preventing and controlling intestinal parasites in yaks in Lhasa, Xizang. Full article

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13 pages, 3971 KiB

Open AccessArticle

Generation and Immunogenicity of Virus-like Particles Based on the Capsid Protein of a Chinese Epidemic Strain of Feline Panleukopenia Virus

by Erkai Feng, Guoliang Luo, Chunxia Wang, Wei Liu, Ruxun Yan, Xue Bai and Yuening Cheng

Vet. Sci. 2025, 12(5), 503; https://doi.org/10.3390/vetsci12050503 - 20 May 2025

Viewed by 395

Abstract

Feline panleukopenia (FPL), caused by the feline panleukopenia virus (FPLV), is a severe and highly contagious viral disease with high morbidity and mortality. Vaccination remains the gold standard for preventing and controlling this debilitating condition. The viral protein VP2 serves as the major [...] Read more.

Feline panleukopenia (FPL), caused by the feline panleukopenia virus (FPLV), is a severe and highly contagious viral disease with high morbidity and mortality. Vaccination remains the gold standard for preventing and controlling this debilitating condition. The viral protein VP2 serves as the major immunogen of FPLV and represents the key target antigen in the development of a novel FPLV vaccine. Virus-like particle (VLP)-based vaccines have emerged as next-generation vaccine candidates due to their high immunogenicity and safe profile. In this study, a baculovirus expression vector system (BEVS) was employed to generate FPLV-VLPs through recombinant expression of the VP2 protein of a Chinese epidemic strain (Ala91Ser, Ile101Thr) of FPLV. The resulting FPLV-VLPs demonstrated markedly enhanced antigenicity and hemagglutination activity, achieving a hemagglutination titer of up to 1:2¹⁶. Following vaccination, immunized cats developed high titers of anti-FPLV hemagglutination inhibition (HI) antibodies (1:2¹⁶) and exhibited 100% protection against challenge with a virulent epidemic FPLV variant (Ala91Ser, Ile101Thr). These findings demonstrate that FPLV-VLPs hold strong potential as candidates for a novel subunit vaccine against FPLV infection. Full article

(This article belongs to the Special Issue Gastrointestinal Disease and Health in Pets)

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15 pages, 1040 KiB

Open AccessArticle

Detection and Comparison of Sow Serum Samples from Herds Regularly Mass Vaccinated with Porcine Reproductive and Respiratory Syndrome Modified Live Virus Using Four Commercial Enzyme-Linked Immunosorbent Assays and Neutralizing Tests

by Chaosi Li, Gang Wang, Zhicheng Liu, Shuhe Fang, Aihua Fan, Kai Chen and Jianfeng Zhang

Vet. Sci. 2025, 12(5), 502; https://doi.org/10.3390/vetsci12050502 - 20 May 2025

Viewed by 424

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) modified live virus (MLV) vaccination is used to control PRRSV. In China, farms conduct random sampling from sow herds every 4 to 6 months. They use the enzyme-linked immunosorbent assay (ELISA) method to monitor the immune [...] Read more.

Porcine reproductive and respiratory syndrome virus (PRRSV) modified live virus (MLV) vaccination is used to control PRRSV. In China, farms conduct random sampling from sow herds every 4 to 6 months. They use the enzyme-linked immunosorbent assay (ELISA) method to monitor the immune status of the herd by tracking the positive rate or the sample-to-positive ratio. However, in farms that implement mass vaccination and have stable production, the positive rate of ELISA antibodies has decreased, especially in high-parity sows. This poses a considerable challenge to the current monitoring approach of PRRSV immunity. It remains unclear whether this reflects insufficient sensitivity of the kits for these special scenarios or the fact that the sows have truly lost immunity. In this study, 233 samples from four farms (A–D) across different regions of China were acquired. They were tested using four representative ELISA kits, two targeting the nucleocapsid protein (N) and two targeting the glycoprotein (GP) to evaluate PRRS immune status. The respective sample positive rates in A–D were 57.1–100%, 50.9–100%, 50–100%, and 75.7–100% using the kits. The positive rates using the four ELISA kits were 50.0–75.7%, 70.0–75.7%, 82.5–97.1%, and 100%, respectively, with poor agreement among them. The positive rates and humoral antibody levels for parity 1 and 2 sows were significantly lower than those with higher parities (>4). Eighty-eight ELISA-negative samples identified using ELISA kit A were verified using a viral neutralizing test (VNT), with only 15.9% of the samples testing negative. In conclusion, the ELISA antibody negativity issue existed, mostly occurring in specific farms tested using a specific kit. However, the low correlation with the VNT results and the poor agreements among the kits suggest that relying on one ELISA test is insufficient to monitor the immune status of PRRSV MLV-vaccinated herds. Full article

(This article belongs to the Special Issue Exploring Innovative Approaches in Veterinary Health)

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20 pages, 3619 KiB

Open AccessCase Report

Vanishing Lung Syndrome in a Dog: Giant Pneumatocele or Giant Pulmonary Bulla Mimicking Tension Pneumothorax—First Report

by Jack-Yves Deschamps, Nour Abboud, Pierre Penaud and Françoise A. Roux

Vet. Sci. 2025, 12(5), 501; https://doi.org/10.3390/vetsci12050501 - 20 May 2025

Viewed by 1044

Abstract

A 6-month-old neutered male Belgian Malinois dog living in a kennel was presented to a veterinary emergency service for the management of severe respiratory distress that had developed within the past 24 h. Thoracic radiographs performed by a referring veterinarian showed abnormalities identified [...] Read more.

A 6-month-old neutered male Belgian Malinois dog living in a kennel was presented to a veterinary emergency service for the management of severe respiratory distress that had developed within the past 24 h. Thoracic radiographs performed by a referring veterinarian showed abnormalities identified as a pneumothorax. Upon admission to the emergency service, the striking anomalies turned out to be a large intrathoracic air-filled cavity and countless smaller ones causing mechanical compression of the adjacent pulmonary parenchyma and mimicking tension pneumothorax. Emergency management included thoracocentesis followed by placement of a thoracostomy tube. The dog exhibited rapid clinical improvement and recovered completely within a few days, without requiring surgical intervention. Serial follow-up radiographs showed progressive and complete resolution of all lesions. Based on the complete resolution without resection, the main lesion—initially interpreted as a giant pulmonary bulla—was ultimately considered consistent with an acquired pneumatocele. To the authors’ knowledge, this is the first report in veterinary medicine of a vanishing lung syndrome presentation in a dog. Full article

(This article belongs to the Special Issue Advancements in Small Animal Internal Medicine)

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12 pages, 521 KiB

Open AccessArticle

Case Study on the Genetic Parameters and Possibilities of Selecting Gilts for Traits Monitored in the Performance Test

by Nenad Stojiljković, Čedomir Radović, Marija Gogić, Vladimir Živković, Aleksandra Petrović, Krstina Zeljić Stojiljković and Dubravko Škorput

Vet. Sci. 2025, 12(5), 500; https://doi.org/10.3390/vetsci12050500 - 20 May 2025

Viewed by 406

Abstract

This research examined the phenotypic and genotypic variability of traits assessed in the gilt performance test and their subsequent impact on gilt selection. The traits evaluated in the gilt performance test were analyzed on two pig farms over a period of 3 consecutive [...] Read more.

This research examined the phenotypic and genotypic variability of traits assessed in the gilt performance test and their subsequent impact on gilt selection. The traits evaluated in the gilt performance test were analyzed on two pig farms over a period of 3 consecutive years. A total of 3664 gilts were included in the research. At the end of the test, body weight, backfat thickness (BF1 and BF2), and longissimus dorsi muscle depth (MLD) were measured using an ultrasound device. The following breeds were evaluated on the farms: Landrace (L)–1981 gilts, Large White (LW)–1344 gilts, and Duroc (D)–339 gilts. In the analyzed population, direct genetic effects accounted for 0.2647 of the total variation in age at the end of the test (AET). Heritability coefficients of 0.37 for BF1 and 0.35 for BF2 indicate that these traits are highly heritable in the studied population. On the other hand, the heritability coefficient for the depth of MLD, which is 0.23, places this trait in the group of medium heritable traits. High heritability coefficients of these traits indicate great potential for genetic improvement through selection. The use of well-designed selection programs aimed at these traits can significantly accelerate the genetic improvement of the population and have an impact on the economic profit of pork production. Full article

(This article belongs to the Special Issue Genetic Improvement and Reproductive Biotechnologies)

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11 pages, 492 KiB

Open AccessArticle

Investigation of Galectin-3 and Cardiotrophin-1 Concentrations as Biomarkers in Dogs with Neurological Distemper

by Alper Erturk, Aliye Sagkan Ozturk and Atakan Ozturk

Vet. Sci. 2025, 12(5), 499; https://doi.org/10.3390/vetsci12050499 - 20 May 2025

Viewed by 432

Abstract

Canine distemper, caused by Morbillivirus canis, is a highly morbid and lethal disease characterized by multiple systemic and neurological signs. In recent years, biomarkers, such as Galectin-3 and Cardiotrophin-1, have been investigated in inflammatory and degenerative diseases. However, the role of these [...] Read more.

Canine distemper, caused by Morbillivirus canis, is a highly morbid and lethal disease characterized by multiple systemic and neurological signs. In recent years, biomarkers, such as Galectin-3 and Cardiotrophin-1, have been investigated in inflammatory and degenerative diseases. However, the role of these biomarkers in neurological distemper has not been investigated. The aim of this study is to compare blood serum Galectin-3 and Cardiotrophin-1 concentrations between the neurological distemper and control group, and to evaluate the correlations of these biomarkers with hematobiochemical parameters in dogs with neurological distemper. Nineteen owned dogs (13 diagnosed with neurological distemper and 6 controls) were included in the study. Hematobiochemical analyses were performed in all dogs, and Galectin-3 and Cardiotrophin-1 concentrations were measured using ELISA. Serum concentrations of Galectin-3 and Cardiotrophin-1 were markedly elevated in dogs with neurological distemper compared to the control group (p < 0.05). A negative correlation between Galectin-3 and monocytes (p < 0.05) and a positive correlation between Galectin-3 and platelet and platelecrit levels (p < 0.05) were observed. There was negative correlation with Cardiotrophin-1 and lymphocyte percentage (p < 0.01) and a positive correlation with Cardiotrophin-1 and granulocyte percentage (p < 0.01). Galectin-3 and Cardiotrophin-1 may serve as biomarkers for the diagnosis and understanding of neurological distemper pathogenesis. Elevated serum concentrations of these biomarkers may indicate underlying neuroinflammation. This may contribute to the pathogenesis of neurological distemper. Full article

(This article belongs to the Section Veterinary Internal Medicine)

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38 pages, 26279 KiB

Open AccessArticle

The Comparative Anatomy of the Metatarsal Foot Pad in Eight Species of Birds of Prey and Owls with Regard to the Development of Pododermatitis

by Rebekka Schwehn, Elisabeth Engelke, Christian Seiler, Dominik Fischer, Hermann Seifert, Christiane Pfarrer, Michael Fehr and Marko Legler

Vet. Sci. 2025, 12(5), 498; https://doi.org/10.3390/vetsci12050498 - 19 May 2025

Viewed by 537

Abstract

Pododermatitis (bumblefoot) occurs commonly in birds of prey and owls and shows species-specific differences in its prevalence, especially between falcons and hawks. The development of the disease is particularly associated with circulatory disorders of the feet. To compare the blood vessel supply of [...] Read more.

Pododermatitis (bumblefoot) occurs commonly in birds of prey and owls and shows species-specific differences in its prevalence, especially between falcons and hawks. The development of the disease is particularly associated with circulatory disorders of the feet. To compare the blood vessel supply of the foot sole, especially the metatarsal foot pad, macroscopic dissections including intravascular injection of latex, contrast µCT scans with barium sulphate, corrosion casts (epoxy resin), and histological examinations of the skin were performed in eight avian species. The main supplying artery of the metatarsal pad, the pulvinar artery, originated from the medial digital artery of the first toe. This main pulvinar artery showed a rather vertical course towards the sole surface, subsequently encircling the metatarsal pad like a basket in falcons and owls, whereas a rather horizontal straight course was observed in northern goshawks and common buzzards. Organized fat tissue was present in the subcutis as the basis for the metatarsal pad only in falcons and owls. The dermis of the metatarsal pad showed a well-developed papillary layer and prominent dermal and subdermal vascular networks in all species examined. The observed differences were discussed regarding both the species-specific prevalence and the etiology of bumblefoot. Full article

(This article belongs to the Section Anatomy, Histology and Pathology)

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15 pages, 261 KiB

Open AccessArticle

The Selection and Training of Shelter Dogs for Involvement in Canine-Assisted Interventions: What Are the Ethical Issues?

by Laura Contalbrigo, Elizabeth A. Walsh, Lieve L. Meers, Daniele Benedetti, Marta De Santis, Emma Bassan and Simona Normando

Vet. Sci. 2025, 12(5), 497; https://doi.org/10.3390/vetsci12050497 - 19 May 2025

Viewed by 520

Abstract

Managing relinquished/stray dogs is a significant socio-economic and ethical challenge necessitating structured policies to ensure animal welfare and integration into society. Critical to successful rehoming is careful assessment of a dog’s temperament/behavior and appropriate matching of a dog to an owner. Shelter dogs’ [...] Read more.

Managing relinquished/stray dogs is a significant socio-economic and ethical challenge necessitating structured policies to ensure animal welfare and integration into society. Critical to successful rehoming is careful assessment of a dog’s temperament/behavior and appropriate matching of a dog to an owner. Shelter dogs’ involvement in canine-assisted interventions (CAIs) provides a potential avenue for rehoming through appropriate dog educational programs. CAIs consist of structured interactions between dog and clients targeted at specific groups with physical/psychological/emotional impairments in various settings. However, there is a lack of standardized protocols for assessing shelter dogs’ suitability for CAI, raising ethical concerns regarding selection/training practices. This study explores the ethical dimensions of rehoming shelter dogs for CAI, employing a philosophical framework considering the responsibilities of humans/veterinarians toward companion animals and how societal perceptions of human–animal relationships impact decision-making in this context. An ethical matrix is applied to evaluate the implications of rehoming decisions on canine and human interests. Our findings highlight the need for a balanced, responsible approach that respects the individuality of dogs while promoting their potential roles in human society. Ethical guidelines for the selection and training of shelter dogs for CAI could enhance their welfare, improve adoption rates, and reinforce the value of the human–animal bond. Full article

(This article belongs to the Section Veterinary Education, Veterinary Communication and Animal Behavior)

15 pages, 1920 KiB

Open AccessArticle

Differential Expression of LHR and FSHR in Canine Mammary Tumors: Correlation with Malignancy and Spay Status

by Yujue Li, Siying Wang, Jiaxuan Gao, Xuerou Tu, Shihui Yu, Yang Liu, Zhaoxia Zhang, Yuan Cui and Yougang Zhong

Vet. Sci. 2025, 12(5), 496; https://doi.org/10.3390/vetsci12050496 - 19 May 2025

Viewed by 627

Abstract

Canine mammary tumors (CMTs) constitute one of the most prevalent malignancies in female canines, whereas the functional involvement of gonadotropin receptors—luteinizing hormone receptor (LHR) and follicle-stimulating hormone receptor (FSHR)—in these neoplasms remains uncharacterized. This study investigated LHR and FSHR expression in 79 CMTs [...] Read more.

Canine mammary tumors (CMTs) constitute one of the most prevalent malignancies in female canines, whereas the functional involvement of gonadotropin receptors—luteinizing hormone receptor (LHR) and follicle-stimulating hormone receptor (FSHR)—in these neoplasms remains uncharacterized. This study investigated LHR and FSHR expression in 79 CMTs and 14 normal mammary tissues collected from 59 female dogs (ethical approval AW82903202-2-3), using immunohistochemistry (IHC) and quantitative real-time PCR, specifically examining their association with tumor malignancy and spay status. Relative to normal mammary tissue, CMTs exhibited statistically significant downregulation of both LHR and FSHR transcripts and corresponding proteins (p < 0.01), with expression progressively decreasing as tumor malignancy increased, and with grade 3 tumors showing almost undetectable levels. Notably, spayed dogs displayed higher LHR levels in tumors (p < 0.05), whereas intact dogs showed higher FSHR levels than spayed dogs. These findings suggest that the expression of LHR and FSHR in CMTs may correlate with tumor malignancy and be influenced by the spay status. Our findings provide the first evidence of gonadotropin receptor dysregulation in CMTs, offering novel insights into their molecular mechanisms and potential applications in hormone-related diagnostic or therapeutic strategies. Full article

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17 pages, 1721 KiB

Open AccessArticle

Subclinical Hypocalcemia Across Lactation Stages Reflects Potential Metabolic Vulnerability in Korean Holstein Cows

by Hector M. Espiritu, Md. Shohel Al Faruk, Hee-Woon Lee, Jaylord M. Pioquinto, Sang-Suk Lee and Yong-Il Cho

Vet. Sci. 2025, 12(5), 495; https://doi.org/10.3390/vetsci12050495 - 19 May 2025

Viewed by 480

Abstract

Subclinical hypocalcemia (SCH) is traditionally associated with the transition period. However, emerging evidence suggests it may persist or recur throughout lactation. This study investigated the prevalence, metabolic profile, and risk factors for SCH in lactating dairy cows in South Korea. A cross-sectional analysis [...] Read more.

Subclinical hypocalcemia (SCH) is traditionally associated with the transition period. However, emerging evidence suggests it may persist or recur throughout lactation. This study investigated the prevalence, metabolic profile, and risk factors for SCH in lactating dairy cows in South Korea. A cross-sectional analysis was conducted on 859 cows from 49 commercial dairy farms. SCH was defined as serum calcium ≤ 8.2 mg/dL. The individual-level prevalence was 9.4%, while 69.4% of herds had at least one affected cow, indicating widespread herd-level occurrence. SCH was significantly associated with parity, with cows in their fourth lactation or higher showing the highest prevalence. Although SCH prevalence did not differ significantly across lactation stages, early lactation cows had significantly lower mean serum calcium concentrations than those in later stages. Milk yield and composition did not differ significantly between groups. However, SCH cows showed notable metabolic differences, including higher serum phosphorus and lower albumin, total protein, and cholesterol levels, indicating potential energy and liver function challenges. Hematological values remained within physiological limits. These findings demonstrate that SCH can persist beyond the transition period, especially in older, high-producing cows, and may remain undetected by routine health checks. Broader monitoring and nutritional support are recommended to enhance metabolic resilience in at-risk animals. Full article

(This article belongs to the Section Nutritional and Metabolic Diseases in Veterinary Medicine)

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19 pages, 971 KiB

Open AccessArticle

Monovalent Copper Oxide in Broiler Nutrition: Effects on Performance, Intestinal Lesions, and Oocyst Shedding During Mild Eimeria Challenge

by Nasima Akter, Thi Hiep Dao, Alip Kumar, David Cadogan, Tamsyn M. Crowley and Amy F. Moss

Vet. Sci. 2025, 12(5), 494; https://doi.org/10.3390/vetsci12050494 - 19 May 2025

Viewed by 470

Abstract

Coccidiosis is a major economic threat in poultry, and with anticoccidials being phased out, cost-effective alternatives like copper (Cu) supplementation are of interest. This study investigated whether in-feed monovalent Cu (100 ppm) could mitigate the effects of a mild Eimeria challenge in broilers. [...] Read more.

Coccidiosis is a major economic threat in poultry, and with anticoccidials being phased out, cost-effective alternatives like copper (Cu) supplementation are of interest. This study investigated whether in-feed monovalent Cu (100 ppm) could mitigate the effects of a mild Eimeria challenge in broilers. A total of 216 broiler chicks were randomly assigned to three treatments (six replicates, 12 birds/replicate): unchallenged control (NC), challenged control (PC), and challenged + Cu-supplemented. Birds were fed starter (days 1–10), grower (days 10–21), and finisher (days 21–35) diets. On day 14, all birds except the NC group were orally challenged with 5000 oocysts each of Eimeria acervulina and E. maxima. Feces were collected (days 17–28) for oocyst count, and growth performance, lesion scores (day 21), carcass traits and bone morphology (day 35), gut morphology (days 21 and 35), gizzard weight (days 21 and 35), and cecal bacterial load (days 21 and 35) were evaluated. The mild challenge was confirmed by the observed differences in lesion scores on day 21 (p < 0.05), fecal oocyst counts from days 17–28 (p < 0.05), and overall mortality (p > 0.05) between challenged and unchallenged groups. Copper supplementation tended to improve FCR during the grower phase (1.403 vs. 1.469; p = 0.057) and significantly reduced oocyst excretion on days 23–25 (p < 0.001) compared to the PC treatment. Although performance benefits were limited, Cu reduced oocyst shedding, indicating potential anticoccidial effects. However, further studies are needed to confirm the consistency of this effect across different doses of Cu in poultry production. Full article

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15 pages, 1031 KiB

Open AccessArticle

Circulating ACTH and Cortisol Investigations in Standardbred Racehorses Under Training and Racing Sessions

by Cristina Cravana, Pietro Medica, Esterina Fazio, Katiuska Satué, Giacoma Brancato, Deborah La Fauci and Giuseppe Bruschetta

Vet. Sci. 2025, 12(5), 493; https://doi.org/10.3390/vetsci12050493 - 19 May 2025

Viewed by 412

Abstract

The hypothalamic-pituitary-adrenal (HPA) axis is a neuroendocrine system involved in the coping response to stressful challenges during exercise stimuli. Exercise represents a significant disruptor of homeostasis, inducing an ACTH-cortisol co-secretion, based on different characteristics of exercise in sport horses. Based on this statement, [...] Read more.

The hypothalamic-pituitary-adrenal (HPA) axis is a neuroendocrine system involved in the coping response to stressful challenges during exercise stimuli. Exercise represents a significant disruptor of homeostasis, inducing an ACTH-cortisol co-secretion, based on different characteristics of exercise in sport horses. Based on this statement, the aim of this study is to evaluate the circulating adrenocorticotropin and cortisol changes in Standardbred trotters, after training and racing sessions, considering the different age and sex. In particular, the aim is to determine to what extent the level of ACTH and cortisol increases during maximum effort in competition conditions (racing), and to compare two exercise conditions of different intensity, training and racing sessions, and effects on ACTH and cortisol responses. Ten Standardbreds, three females and seven males, clinically healthy, were enrolled and subjected to two exercise conditions: a non-competitive session (training) and then a competitive event (racing). Four of them were 2-year-olds and a further six were 3-year-olds. Training and racing effects on both ACTH (p < 0.01) and cortisol (p < 0.01) values were obtained. Compared to the training session, horses showed greater ACTH concentrations at rest (p < 0.001), at 5 (p < 0.01) and 30 min (p < 0.001), and lower cortisol concentrations only at rest (p < 0.01) after racing; 2- and 3-year-old horses showed the greater ACTH concentrations at 5 and 30 min (p < 0.01) post-racing; males showed the greater ACTH concentrations at 5 min and 30 min (p < 0.01) post-racing. The different stimuli of the two contexts, and differences in exercise intensity, such as training and competitive event, may have affected the direction of hypothalamic-pituitary-adrenal (HPA) axis response, both as an ability to adapt to physical stress of different intensity and as a preparatory activity for coping with stimuli. In conclusion, training and racing events induced a different HPA axis response in which both emotional experience and physical maturity could induce a significant adaptive response. As ACTH and cortisol concentrations in adult equids are extremely heterogeneous, further investigation is required to explore how different variables can influence the hormonal dynamics and their role as expressions of adaptive strategies to stress in horses. Full article

(This article belongs to the Special Issue The Progress of Equine Medical Research in China and Beyond)

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15 pages, 5379 KiB

Open AccessArticle

Activity of Different Types of Cactus Forage on Testicular Function and Morphology of Sheep Subjected to Environmental Heat Stress

by Giselle Woolley Cardoso da Silva, Fernanda Carolina Ribeiro Dias, Marli do Carmo Cupertino, Alluanan Adelson do Nascimento Silva, Ângela Maria Vieira Batista, Emanuel Felipe de Oliveira Filho, Francisco Fernando Ramos de Carvalho, Ana Lúcia Figueiredo Porto and Valdemiro Amaro da Silva Júnior

Vet. Sci. 2025, 12(5), 492; https://doi.org/10.3390/vetsci12050492 - 19 May 2025

Viewed by 409

Abstract

In this study, we aim to analyze whether supplementation with cactus has the potential to minimize the testicular damage caused by heat stress, assess which of the three cactuses would have the best potential, and suggest a possible pathway (oxidative or hormonal) for [...] Read more.

In this study, we aim to analyze whether supplementation with cactus has the potential to minimize the testicular damage caused by heat stress, assess which of the three cactuses would have the best potential, and suggest a possible pathway (oxidative or hormonal) for the action of cactus on the testicular parameter alterations caused by heat stress. Thirty-two male lambs, of the Santa Inês type, not castrated, approximately six months old, and averaging 21.0 ± 2.0 kg body weight, were divided into four groups, as follows: G1, which was fed an elephant grass hay diet (control); G2, with a diet with partial replacement using small cactus forage (Opuntia cochenillifera) (SMALL); G3, which was fed Mexican elephant ear (Opuntia stricta Haw) (MEE); and G4, which was fed IPA Sertania (Nopalea cochenillifera Salm Dyck) (IPA) for 63 days. After slaughter, blood and testicles were removed. The right testicles were fixed for histological analyses, and the left testicles were stored in the freezer for oxidative stress analyses. Serum testosterone, T3, and T4 levels were analyzed. The body weight of animals treated with cactus forage was higher than in the control group. However, the gonadosomatic index did not differ among experimental groups. Heat stress triggered the degradation of testis tissue in all experimental groups. The testicular degeneration process was characterized by tubular atrophy, reduction in germ epithelium height, germ cell vacuolization and necrosis, Sertoli cell vacuolization, germ cell scaling of the tubular fire, and increased intertubular space. The three different cactus forages used in this study had different weaknesses regarding their antioxidant defenses, hormonal levels, and histopathology. However, it is important to highlight that the IPA group had lower qualitative changes in the intertubular areas than the other experimental groups. The testosterone level increased in MEE (Opuntia stricta) and IPA groups, while T3 and T4 increased in SMALL (Opuntia cochenillifera) and IPA groups. The malondialdehyde, an important marker of lipid peroxidation, was reduced only in the IPA group. The testosterone level increased in MEE and IPA groups, while T3 and T4 increased in SMALL and IPA groups. In conclusion, heat stress triggers several histopathologies in testis tissue, and IPA cactus (Nopalea cochenillifera) was the most appropriate supplementation for reducing the damages, compared with an elephant grass hay diet or small cactus forage and Mexican elephant ear supplementation. Full article

(This article belongs to the Section Anatomy, Histology and Pathology)

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17 pages, 697 KiB

Open AccessArticle

Telomere Tales: Exploring the Impact of Stress, Sociality, and Exercise on Dogs’ Cellular Aging

by Luisa Mascarenhas Ladeia Dutra, Flaviane S. Souza, Angelica Silva Vasconcellos, Robert J. Young and Ivana Gabriela Schork

Vet. Sci. 2025, 12(5), 491; https://doi.org/10.3390/vetsci12050491 - 19 May 2025

Viewed by 861

Abstract

Animal welfare is influenced by the cumulative life experiences of an individual. Among these, exposure to chronic stressors has a significant impact on both physical and mental health, contributing to premature aging—a process linked to telomere shortening. Conversely, positive experiences have been shown [...] Read more.

Animal welfare is influenced by the cumulative life experiences of an individual. Among these, exposure to chronic stressors has a significant impact on both physical and mental health, contributing to premature aging—a process linked to telomere shortening. Conversely, positive experiences have been shown to mitigate, delay, and sometimes reverse telomere attrition. This suggests that telomere length could be a reliable indicator for assessing animal welfare. This study explored the association between telomere length and characteristics such as life history, environment, and health in domestic dogs. Buccal swabs collected DNA samples from 250 dogs, and telomere length was quantified via qPCR. Our findings revealed that environmental factors significantly influenced telomere length. Dogs housed in kennels or subjected to low physical activity levels exhibited shorter telomeres. Similarly, dogs living in groups of more than five dogs had shorter telomeres, and male dogs were found to have longer telomeres than females. Overall, these results highlight the importance of environmental conditions in influencing telomere length in dogs and the potential to use this biological indicator to evaluate animal welfare. Full article

(This article belongs to the Special Issue Advanced Therapy in Companion Animals)

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12 pages, 1190 KiB

Open AccessArticle

Autophagic Degradation of GPX4 Mediates Ferroptosis During Sheep Sperm Cryopreservation

by Boyuan Li, Erhan Hai, Yukun Song and Jiaxin Zhang

Vet. Sci. 2025, 12(5), 490; https://doi.org/10.3390/vetsci12050490 - 19 May 2025

Viewed by 521

Abstract

Ferroptosis is implicated in cryodamage to sheep sperm, potentially due to glutathione peroxidase 4 (GPX4) degradation during freezing; however, the pathway underlying GPX4 degradation remains unclear. In this study, a comparison of cryoprotective effects between the autophagy inhibitor chloroquine (CQ) and the ubiquitination [...] Read more.

Ferroptosis is implicated in cryodamage to sheep sperm, potentially due to glutathione peroxidase 4 (GPX4) degradation during freezing; however, the pathway underlying GPX4 degradation remains unclear. In this study, a comparison of cryoprotective effects between the autophagy inhibitor chloroquine (CQ) and the ubiquitination inhibitor MG132 revealed that 5 μM CQ treatment significantly enhanced the motility (p < 0.01) and sperm plasma membrane integrity rate (p < 0.01) of frozen–thawed sperm; no protective effects were observed in any MG132 treatment group. Mechanistic analysis indicated that CQ treatment substantially restored GPX4 protein expression (p < 0.01), and concurrently reduced lipid peroxidation (p < 0.01) and free iron ion accumulation (p < 0.01), in frozen–thawed sperm. These findings suggest that GPX4 degradation during cryopreservation occurs via the autophagy pathway. This study established a ferroptosis–GPX4–autophagy axis during sheep sperm cryopreservation and identified autophagy-mediated GPX4 loss as a potential target for enhancing sperm cryoprotection. Full article

(This article belongs to the Special Issue Advances in Animal Sperm Conservation Techniques for Better Fertility—2nd Edition)

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12 pages, 3092 KiB

Open AccessArticle

Bacillus subtilis Fed to Sows Promotes Intestinal Development and Regulates Mucosal Immunity in Offspring

by Peng Liu, Jinjiao Zuo, Hui Lu, Bin Zhang and Caihong Wu

Vet. Sci. 2025, 12(5), 489; https://doi.org/10.3390/vetsci12050489 - 18 May 2025

Viewed by 423

Abstract

Diarrhea in piglets causes intestinal inflammation and epithelial damage. Weaned piglets fed with Bacillus subtilis (B.S) have enhanced intestinal mucosal immunity and reduces diarrhea in piglets. However, the immune system of newborn piglets is immature, and B.S cannot effectively activate the intestinal mucosal [...] Read more.

Diarrhea in piglets causes intestinal inflammation and epithelial damage. Weaned piglets fed with Bacillus subtilis (B.S) have enhanced intestinal mucosal immunity and reduces diarrhea in piglets. However, the immune system of newborn piglets is immature, and B.S cannot effectively activate the intestinal mucosal reaction when given directly. This research explored the impact of the maternal supplementation of B.S-Dia during the final 35 days of gestation on piglet intestinal development and mucosal immunity. The results demonstrated that B.S-Dia administration significantly increased the body weight, jejunal villus height, and crypt depth in the piglets. In addition, B.S-Dia also significantly increased the proliferative activity of intestinal epithelial cells, as evidenced by proliferating cell nuclear antigen (PCNA) staining and the elevated mRNA expression of the proliferation-related gene (c-Myc). Furthermore, B.S-Dia supplementation also reinforced the intestinal mucosal barrier by increasing goblet cell numbers and upregulating the mRNA expression of antimicrobial peptides, such as Muc2 and Lyz-1. Finally, elevated levels of IL-4 and IFN-γ, along with an increased abundance of CD3⁺ T cells, revealed that the intestinal mucosal immunity of piglets was improved after B.S-Dia administration. Our study indicates that feeding B.S-Dia to sow spromotes intestinal development and improves intestinal mucosal immunity in piglets. Full article

(This article belongs to the Section Veterinary Microbiology, Parasitology and Immunology)

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14 pages, 859 KiB

Open AccessArticle

The Suitability of Dried Blood Spot Sampling for Pharmacokinetic Studies in Veterinary Medicine

by Anisa Bardhi, Andrea Barbarossa, Andrè Joubert, Ronette Gehring, Carlotta Lambertini and Noemi Romagnoli

Vet. Sci. 2025, 12(5), 488; https://doi.org/10.3390/vetsci12050488 - 18 May 2025

Viewed by 467

Abstract

Dried blood spot (DBS) sampling has emerged as a promising microsampling technique in biomedical and clinical research, offering advantages such as reduced invasiveness, minimal blood volume requirements, and enhanced analyte stability. Although well established in human medicine for neonatal screening and diagnostic applications, [...] Read more.

Dried blood spot (DBS) sampling has emerged as a promising microsampling technique in biomedical and clinical research, offering advantages such as reduced invasiveness, minimal blood volume requirements, and enhanced analyte stability. Although well established in human medicine for neonatal screening and diagnostic applications, its potential in veterinary pharmacology remains underexplored. This study investigated the feasibility of using DBS samples to quantify anesthetic agents—ketamine and medetomidine in cats and lidocaine in horses—during routine surgical procedures at a veterinary teaching hospital. A standardized DBS collection protocol was developed, and LC-MS/MS methods were validated for the quantification of target analytes in both DBS and plasma samples. These methods were subsequently applied to real samples collected during anesthesia to conduct pharmacokinetic analyses. Comparative evaluations, including Bland–Altman analysis, assessed the suitability of DBS samples for pharmacokinetic studies in veterinary medicine. Preliminary results indicated satisfactory agreement for medetomidine, meeting EMA guidelines, with 75.6% of mean values falling within ±20% of paired measurements. Results for ketamine (46.9%) were promising but require further optimization, while those for lidocaine (21.4%) highlighted the need for additional investigation. These findings underscore the potential of DBS sampling as a minimally invasive alternative for pharmacokinetic studies in veterinary medicine, particularly for medetomidine, while identifying areas for further methodological refinement. Future research should optimize DBS techniques and expand their application to other drugs and species, broadening their impact on veterinary pharmacology. Full article

(This article belongs to the Section Veterinary Physiology, Pharmacology, and Toxicology)

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21 pages, 2560 KiB

Open AccessArticle

Clinical Relevance of Distinguishing Between Three Endoscopy-Based Conditions, Bronchiectasis, Bronchomalacia, and Their Combination in Dogs: A Retrospective Study

by Aurélie Lyssens, Géraldine Bolen, Aline Fastrès, Cécile Clercx and Frédéric Billen

Vet. Sci. 2025, 12(5), 487; https://doi.org/10.3390/vetsci12050487 - 18 May 2025

Viewed by 602

Abstract

Bronchiectasis (BE) and bronchomalacia (BM) are chronic respiratory diseases in dogs, yet their combined occurrence (BEBM) is not well studied. This retrospective study analyzed 65 dogs diagnosed via endoscopy with BE, BM, or BEBM (E-BE, E-BM, E-BEBM) to identify clinical and pathological differences [...] Read more.

Bronchiectasis (BE) and bronchomalacia (BM) are chronic respiratory diseases in dogs, yet their combined occurrence (BEBM) is not well studied. This retrospective study analyzed 65 dogs diagnosed via endoscopy with BE, BM, or BEBM (E-BE, E-BM, E-BEBM) to identify clinical and pathological differences and assess how imaging results (radiography and computed tomography (CT)) align with endoscopic findings. Clinical symptoms like coughing, dyspnea, and exercise intolerance were similar across all groups, except lung crackles, which were more common in E-BEBM. Inflammation seen during bronchoscopy and bronchoalveolar lavage fluid results, including neutrophil counts, showed no significant differences between groups. Bacterial infections were present in 15% of dogs with no difference among groups. Diagnostic agreement between radiography and endoscopy was low: 18.1% for E-BE, 10.5% for E-BM, and 38.4% for E-BEBM. CT results matched endoscopic findings in all E-BE cases but only in half of E-BM and 40% of E-BEBM cases. The bronchial-to-arterial ratio, a benchmark for BE diagnosis, did not align with CT findings. Overall, the study found limited clinical or pathological differences between BE, BM, and BEBM and limited concordance between imaging and endoscopic findings, emphasizing the need for further research to clarify potential implications for treatment strategies. Full article

(This article belongs to the Section Veterinary Internal Medicine)

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21 pages, 1143 KiB

Open AccessArticle

Evaluation of Plasma Nitric Oxide and Serum Endothelial Nitric Oxide Synthase in Pulmonary Hypertensive Dogs: A Clinical and Echocardiography Investigation

by Siwayu Rattanakanokchai, Numfa Fungbun, Ketmanee Senaphan, Supranee Jitpean and Trasida Ployngam

Vet. Sci. 2025, 12(5), 486; https://doi.org/10.3390/vetsci12050486 - 16 May 2025

Viewed by 434

Abstract

Nitric oxide (NO), an endogenous vasodilator, has been proposed as a biomarker for pulmonary hypertension (PH) in humans. NO is synthesized by endothelial nitric oxide synthase (eNOS). Alterations in NO/eNOS have not been studied in dogs with PH. We assessed alterations in NO [...] Read more.

Nitric oxide (NO), an endogenous vasodilator, has been proposed as a biomarker for pulmonary hypertension (PH) in humans. NO is synthesized by endothelial nitric oxide synthase (eNOS). Alterations in NO/eNOS have not been studied in dogs with PH. We assessed alterations in NO and eNOS in the blood of dogs with PH (n = 17) and healthy dogs (n = 10) and analyzed their correlations with echocardiographic parameters. The results showed significantly higher plasma NO and serum eNOS levels in dogs with PH compared with healthy dogs. Dogs with PH and ascites (n = 11) had significantly lower plasma NO levels than those without ascites (n = 6) and presented a decreasing eNOS trend. In dogs with PH, plasma NO was positively correlated with left ventricular hemodynamics, right ventricular compliance, and pulmonary distensibility, but was negatively correlated with pulmonary vascular resistance and right cardiac remodeling. Serum eNOS was positively correlated with the main pulmonary artery diameter. Increments in NO/eNOS reflected compensatory responses to cardiovascular changes in PH. These compensations were downward in the advanced stages. Other factors may also impact NO/eNOS compensation. Although the role of NO/eNOS as biomarkers for PH in dogs remains equivocal, they may indicate compensatory consequences of cardiovascular alterations. Full article

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20 pages, 602 KiB

Open AccessArticle

Veterinary Perspectives on Hemoglobin-Based Oxygen Carriers in Experimental Hemorrhagic Shock: Insights from Rabbit Models

by Ștefania-Mădălina Dandea, Alina-Diana Hașaș, Vlad-Alexandru Toma, Maria Lehene, Florina Scurtu, Cosmin Petru Peștean, Răzvan-Andrei Codea, Lucia-Victoria Bel, Iulia Melega, Radu Silaghi-Dumitrescu and Bogdan Sevastre

Vet. Sci. 2025, 12(5), 485; https://doi.org/10.3390/vetsci12050485 - 16 May 2025

Viewed by 1025

Abstract

Hemoglobin-based oxygen carriers (HBOCs) represent a promising alternative to traditional blood transfusions, offering the advantages of extended shelf life and avoiding blood compatibility limitations and infection risks. Positive effects of hemoglobin-based oxygen carriers (HBOCs) on hemorrhagic shock have been researched across various animal [...] Read more.

Hemoglobin-based oxygen carriers (HBOCs) represent a promising alternative to traditional blood transfusions, offering the advantages of extended shelf life and avoiding blood compatibility limitations and infection risks. Positive effects of hemoglobin-based oxygen carriers (HBOCs) on hemorrhagic shock have been researched across various animal species, including swine, rats, rabbits, guinea pigs, and dogs. As previously described, HBOCs based on ovine hemoglobin display better efficiency in the context of hemorrhagic shock compared to those based on the more commonly used bovine hemoglobin. This was evidenced through higher survival rates and more favorable histopathological and immunological outcomes. The vascular effects of ovine hemoglobin polymerized with glutaraldehyde exposure included the absence of hypertension, minimal endothelial damage with slight alterations in inducible nitric oxide synthase (iNOS), and reduced vascular inflammation mediated by interleukin-10 (IL-10). Ovine hemoglobin has emerged as a particularly promising raw material for the development of HBOCs, surpassing bovine and human hemoglobin due to its advantages in availability and efficacy. Furthermore, reducing oxidative stress by polymerizing hemoglobin with glutaraldehyde is most effective with ovine hemoglobin compared to bovine hemoglobin. This study evaluates the effectiveness of ovine hemoglobin polymerized with glutaraldehyde in managing hemorrhagic shock in rabbits, with a focus on its ability to maintain blood pressure, support oxygen transport, and assess potential systemic and oxidative responses. Fifteen adult New Zealand white rabbits, divided into three equal groups, were included in this study: a negative control group transfused with colloid solutions, a positive control group treated with autotransfusion, and a group receiving HBOCs. All groups underwent a hemorrhagic shock protocol, with 40% of their total blood volume withdrawn under deep anesthesia, followed by transfusions 30 min later. Vital parameters, including invasive arterial blood pressure, heart rate, and end-tidal CO₂, were measured throughout the experimental procedures. Arterial blood gas samples were collected before the procedures, after hemorrhagic shock induction, and at the conclusion of the transfusion. In summary, HBOCs offer a promising solution for oxygen delivery, but their effects on blood chemistry, particularly CO₂ and lactate levels, must be considered. Although no direct oxygenation issues were observed in experimental models, elevated CO₂ levels and the interference of HBOCs with lactate measurements emphasize the importance of vigilant clinical monitoring. Polymerized hemoglobin provides a non-nephrotoxic alternative, but challenges persist in preventing nitric oxide scavenging and ensuring effective oxygen delivery. Full article

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16 pages, 4625 KiB

Open AccessArticle

Lactobacillus Re-Engineers Gut Microbiota to Overcome E. coli Colonization Resistance in Mice

by Jianlei Jia, Pengjia Bao, Qinran Yu, Ning Li, Hao Ren, Qian Chen and Ping Yan

Vet. Sci. 2025, 12(5), 484; https://doi.org/10.3390/vetsci12050484 - 16 May 2025

Viewed by 482

Abstract

The intestinal health and functionality of animals play pivotal roles in nutrient digestion and absorption, as well as in maintaining defense against pathogenic invasions. These biological processes are modulated by various determinants, including husbandry conditions, dietary composition, and gut microbial ecology. The excessive [...] Read more.

The intestinal health and functionality of animals play pivotal roles in nutrient digestion and absorption, as well as in maintaining defense against pathogenic invasions. These biological processes are modulated by various determinants, including husbandry conditions, dietary composition, and gut microbial ecology. The excessive use of anthropogenic antibiotics may disrupt intestinal microbiota composition, potentially leading to dysbiosis that directly compromises host homeostasis. While Lactobacillus species are recognized for their immunomodulatory properties, their precise mechanisms in regulating host anti-inflammatory gene expression and influencing mucosal layer maturation, particularly regarding E. coli colonization resistance, require further elucidation. To investigate the regulatory mechanisms of Lactobacillus in relation to intestinal architecture and function during E. coli infection, we established a colonic infection model using Bal b/c mice, conducting systematic analyses of intestinal morphology, inflammatory mediator profiles, and microbial community dynamics. Our results demonstrate that Lactobacillus supplementation (Pediococcus acidilactici) effectively mitigated E. coli O78-induced enteritis, with co-administration during infection facilitating the restoration of physiological parameters, including body mass, intestinal histoarchitecture, and microbial metabolic functions. Microbiome profiling revealed that the Lactobacillus intervention significantly elevated Lactococcus abundance while reducing Weissella populations (p < 0.05), concurrently enhancing metabolic pathways related to nutrient assimilation and environmental signal processing (including translation mechanisms, ribosomal biogenesis, amino acid transport metabolism, and energy transduction systems; p < 0.05). Mechanistically, Lactobacillus administration attenuated E. coli-induced intestinal pathology through multiple pathways: downregulating pro-inflammatory cytokine expression (IL-1β, IL-1α, and TNF-α), upregulating epithelial junctional complexes (Occludin, Claudin-1, and ZO-1), and stimulating mucin biosynthesis (MUC1 and MUC2; p < 0.05). These modifications collectively enhanced mucosal barrier integrity and promoted epithelial maturation. This investigation advances our comprehension of microbiota–host crosstalk during enteropathogenic infections under probiotic intervention, offering valuable insights for developing novel nutritional strategies and microbial management protocols in animal husbandry. Full article

(This article belongs to the Topic Application of Animal Models: From Physiology to Pathology)

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15 pages, 5760 KiB

Open AccessArticle

Pathological Characteristics of Pregnant Tree Shrews Infected by Zoonotic Hepatitis E Virus Genotype and the Effect of Estrogen on Virus Replication

by Peiying Zhu, Guojun Wang, Veerasak Punyapornwithaya, Chalita Jainonthee, Jijing Tian, Yan Liu, Fanan Suksawat, Sunpetch Angkititrakul, Yuchen Nan, Zailei Li, Xinhui Duan and Wengui Li

Vet. Sci. 2025, 12(5), 483; https://doi.org/10.3390/vetsci12050483 - 16 May 2025

Viewed by 450

Abstract

Hepatitis E, caused by the hepatitis E virus (HEV), is a zoonotic disease that extends beyond hepatocellular necrosis to replicate in multiple organs. While most infections are self-limiting, HEV infection during pregnancy is associated with severe outcomes, including acute liver failure, preterm delivery, [...] Read more.

Hepatitis E, caused by the hepatitis E virus (HEV), is a zoonotic disease that extends beyond hepatocellular necrosis to replicate in multiple organs. While most infections are self-limiting, HEV infection during pregnancy is associated with severe outcomes, including acute liver failure, preterm delivery, and miscarriage, with the mechanisms underlying this high pathogenicity remaining poorly understood. This study established a pregnant tree shrew model with a late-stage HEV infection and a cellular model using zoonotic HEV genotypes GT3 and GT4 to investigate the effects of estrogen on HEV replication. Results showed that negative-strand RNA detection revealed replicative intermediates in feces and tissues during the acute phase, with peak viral loads occurring within one week and the highest titers in bile. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels rose at 3 days post-inoculation (DPI), peaking at 7 DPI. Elevated estrogen levels post-miscarriage correlated with increased viral loads, a trend mirrored in cell culture models showing linear relationships between estrogen and viral replication. Histopathology demonstrated viral hepatitis lesions in liver tissues and abnormalities in the uterus, ovaries, and brain, including hydropic degeneration, neuronal disruption, and granulosa cell necrosis. This study developed a pregnant tree shrew model for HEV infection, providing a robust tool for exploring pathogenic mechanisms during pregnancy and genotype-specific differences in zoonotic HEV pathogenicity. These findings offer new insights into the role of estrogen in HEV replication and its contribution to adverse pregnancy outcomes. Full article

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16 pages, 1149 KiB

Open AccessArticle

Post-Insemination Infusion of Wharton’s Jelly Mesenchymal Stromal/Stem Cells-Derived Conditioned Medium: A Novel Approach for Improving Pregnancy Outcomes in Problem Mares

by Chiara Del Prete, Emilia Attolini, Barbara Merlo, Eleonora Iacono, Francesca Paola Nocera, Luisa De Martino, Consiglia Longobardi, Sara Damiano, Valentina Longobardi, Natascia Cocchia and Maria Pia Pasolini

Vet. Sci. 2025, 12(5), 482; https://doi.org/10.3390/vetsci12050482 - 16 May 2025

Viewed by 685

Abstract

Treating endometritis is one of the major challenges faced by veterinarians. The effect of post-insemination intrauterine infusion Wharton’s jelly mesenchymal stromal/stem cell-derived conditioned medium (WJ-MSC-CM) on the uterine response and pregnancy outcomes in problem mares was evaluated in this study. The cycles of [...] Read more.

Treating endometritis is one of the major challenges faced by veterinarians. The effect of post-insemination intrauterine infusion Wharton’s jelly mesenchymal stromal/stem cell-derived conditioned medium (WJ-MSC-CM) on the uterine response and pregnancy outcomes in problem mares was evaluated in this study. The cycles of 12 problem mares were treated seven/eight hours after insemination with intrauterine infusion of 20 mL of WJ-MSC-CM (TRT) or non-conditioned Ringer’s lactate solution (CTR). Before (PRE) and 12 h after (POST) treatment, an ultrasonographic examination and a low-volume flush (LVF) for efflux clarity evaluation and bacteriological and cytological analysis were made. Pregnancy diagnosis was performed at 14 days and confirmed at 60 days after ovulation. A decrease (p < 0.05) in polymorphonuclear neutrophils infiltration and a trend toward significance in LVF efflux clarity (p < 0.1) were observed in TRT cycles. The TRT (50%) had a higher pregnancy rate (p < 0.05) than CTR group (0%) at first cycle. Only a trend toward significance was observed comparing pregnancy frequencies between the two groups (CTR 12.5% vs. TRT 54.5%), the pregnancy rate of TRT cycles was comparable to that of normal mares (p > 0.05) and higher (p < 0.05) than that reported for problem mares in the literature. Post-insemination uterine infusion of WJ-MSC-CM in mares restores pregnancy rates to normal levels, by its anti-inflammatory effect. Full article

(This article belongs to the Section Veterinary Reproduction and Obstetrics)

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16 pages, 2389 KiB

Open AccessArticle

Molecular Prevalence and Phylogenetic Analysis of Anaplasma spp. in Goats from Adana, Türkiye

by Tülin Güven Gökmen, Armağan Erdem Ütük, Esra Aslan Tokgöz, Nur Sima Uprak, Afra Sena Tekin, Ufuk Erol, Pınar Ayvazoğlu Demir, Osman Sezer and Elçin Günaydın

Vet. Sci. 2025, 12(5), 481; https://doi.org/10.3390/vetsci12050481 - 15 May 2025

Viewed by 698

Abstract

Anaplasmosis is a disease in animals that leads to significant economic losses. In addition, the zoonotic potential of vector-borne Anaplasma species is increasing its importance, both around the world and in Türkiye, in particular. The aim of this study was to determine the [...] Read more.

Anaplasmosis is a disease in animals that leads to significant economic losses. In addition, the zoonotic potential of vector-borne Anaplasma species is increasing its importance, both around the world and in Türkiye, in particular. The aim of this study was to determine the prevalence and phylogenetic profile of Anaplasma species in goats from the districts of Adana province, Türkiye, via molecular methods. For this purpose, blood samples from 364 goats of various breeds, ages, and genders were collected, and the presence of 55 Anaplasma spp. was determined through the PCR method. Anaplasma ovis was detected in 55 samples (15.1%) and A. phagocytophilum in 11 samples (3%) by Nested-PCR. However, A. capra was not detected. The phylogenetic relationships of A. ovis and A. phagocytophilum were investigated by DNA sequencing of the msp4 and 16S rRNA gene regions. Through sequence analysis in A. phagocytophilum, we identified nine isolates as A. phagocytophilum-like 1 and one isolate as A. phagocytophilum-like 2. Forty-six of the A. ovis isolates were 100% similar to each other. The other 2 isolates were also 100% similar to each other but had a single nucleotide difference from the other 46 isolates. Notably, the isolate identified as A. phagocytophilum-like 2 was detected for the first time in goats in Türkiye. Full article

(This article belongs to the Section Veterinary Microbiology, Parasitology and Immunology)

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14 pages, 1281 KiB

Open AccessArticle

Epidemiological Studies on Eye Diseases in Centers for Stray Dogs in Northwestern Romania

by Elena Bonea, Cornel Dionisie Igna, Monica Ocnean, Bianca Cornelia Lungu and Ioan Hutu

Vet. Sci. 2025, 12(5), 480; https://doi.org/10.3390/vetsci12050480 - 15 May 2025

Viewed by 461

Abstract

Eye disease in dogs is a common problem that can affect both the health and well-being of the animals. The aim of the study was to investigate the prevalence and incidence of the most common eye diseases in a large population of dogs [...] Read more.

Eye disease in dogs is a common problem that can affect both the health and well-being of the animals. The aim of the study was to investigate the prevalence and incidence of the most common eye diseases in a large population of dogs from two stray dog centers in northwestern Romania over a two-year period.We conducted a retrospective observational study of 208 eye diseases in two dog shelters in northwestern Romania: the “Ham Ham” Association and the “Free Life” Association in Satu Mare County. The study spanned from January 2022 to December 2023. We collected data on eye disease status from a total of 2293 dogs. The dogs were categorized into three age groups: young dogs (under 2 years), adult dogs (2 to 7 years), and senior dogs (over 7 years). Additionally, the dogs were grouped by sterilization status and gender. The statistical analysis was performed using Pearson’s chi-squared test, Student’s t-test, and binary logistic regression. The prevalence of ocular diseases in this study was 9.07%, with 208 out of 2293 dogs diagnosed with eye problems. The most common ocular diseases observed were conjunctivitis (58/208 or 27.9%), eyelid issues (50/208 or 24%), cataracts (40/208 or 19.2%), glaucoma (13/208 or 6.3%), keratopathies (10/208 or 4.8%), traumatic eye injuries (10/208 or 4.8%), foreign bodies (7/208 or 3.4%), blepharitis (5/208 or 2.4%), proptosis (3/208 or 1.4%), progressive retinal atrophy ((3/208 or 1.4%), retinal detachment ((3/208 or 1.4%), and dry eye (2/208 or 1%). Among the three age groups, puppies and young dogs (under 2 years) were the most affected, with a punctual prevalence of 50.96% at the “Free Life” center and 65.51% at the “Ham Ham” center. Compared with older dogs, the younger dogs were 4.91 times more likely to develop conjunctivitis (OR = 4.91, p = 0.001), 9.38 times more likely to develop eyelid problems (OR = 9.38, p < 0.000), and 0.31 times less likely to develop cataracts (OR = 0.31, p = 0.019).Our epidemiological study found that eye diseases are common among shelter dogs, with incidence rates (new cases over a specific period) varying by age. The youngest and oldest dogs were the most affected, with the highest prevalence observed in the youngest group. The study also highlighted the significant impact of factors such as the shelter center (p < 0.000), age (p < 0.000), and sterilization status (p < 0.000) on the prevalence of eye disorders. These findings underscore the need for enhanced awareness about eye diseases and their risk factors to facilitate early diagnosis and treatment. Full article

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22 pages, 7012 KiB

Open AccessArticle

Multi-Omics Unveils Inflammatory Regulation of Fermented Sini Decoction Dregs in Broilers Infected with Avian Pathogenic Escherichia coli

by Shuanghao Mo, Xin Fang, Wenxi Xiao, Bowen Huang, Chunsheng Li, Hui Yang, Yilin Wu, Yiming Wang and Hongxia Ma

Vet. Sci. 2025, 12(5), 479; https://doi.org/10.3390/vetsci12050479 - 15 May 2025

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Abstract

Avian colibacillosis causes significant economic losses and raises concerns for human health due to food safety risks, a problem exacerbated by the increase in antibiotic resistance. This study aimed to develop novel antibacterial strategies using fermented liquid of Sini decoction dregs to address [...] Read more.

Avian colibacillosis causes significant economic losses and raises concerns for human health due to food safety risks, a problem exacerbated by the increase in antibiotic resistance. This study aimed to develop novel antibacterial strategies using fermented liquid of Sini decoction dregs to address these challenges. We analyzed the transcriptome of the chicken thymus sample GSE69014 in the GEO database to identify immune-related genes, performed molecular docking to assess compound interactions, and experimental validation via Western blot and ELISA to evaluate anti-inflammatory effects. Results revealed 11 core genes, including TLR4, critical for immune responses against the infection, with TLR4 activating key inflammatory pathways. Fermented liquid with probiotics enhanced bioactivity, and natural compounds Dioscin and Celastrol from the fermented liquid inhibited inflammation by targeting the TLR4-MD2 complex. In animal models, fermented liquid outperformed individual compounds, likely due to synergistic effects, significantly reducing inflammatory markers. These findings demonstrate that fermented liquid of Sini decoction dregs offers a promising, sustainable approach to control avian colibacillosis, mitigate antibiotic resistance, and improve poultry health, providing a scientific foundation for its application in farming to reduce economic losses and enhance food safety. Full article

(This article belongs to the Topic Recent Advances in Veterinary Pharmacology and Toxicology)

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Vet. Sci., Volume 12, Issue 5 (May 2025) – 115 articles

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