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Article

Genetic Variation of Native Perilla Germplasms Collected from South Korea Using Simple Sequence Repeat (SSR) Markers and Morphological Characteristics

by 1,2,†, 1,†, 1,2,†, 3, 3, 3 and 1,2,*
1
Department of Applied Plant Sciences, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 24341, Korea
2
Interdisciplinary Program in Smart Agriculture, Kangwon National University, Chuncheon 24341, Korea
3
National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA, Jeonju 54874, Korea
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editors: Mario Ciaffi and Toyoaki Anai
Plants 2021, 10(9), 1764; https://doi.org/10.3390/plants10091764
Received: 21 July 2021 / Revised: 17 August 2021 / Accepted: 20 August 2021 / Published: 25 August 2021
(This article belongs to the Special Issue Genetic Diversity and Molecular Breeding in Plants)
Using morphological characteristics and simple sequence repeat (SSR) markers, we evaluated the morphological variation and genetic diversity of 200 Perilla accessions collected from the five regions of South Korea and another region. In morphological characteristics analysis, particularly leaf color, stem color, degree of pubescence, and leaf size have been found to help distinguish the morphological features of native Perilla accessions cultivated in South Korea. Twenty SSR primer sets confirmed a total of 137 alleles in the 200 Perilla accessions. The number of alleles per locus ranged from 3 to 13, with an average number of alleles per locus of 6.85. The average genetic diversity (GD) was 0.649, with a range of 0.290–0.828. From analysis of SSR markers, accessions from the Jeolla-do and Gyeongsang-do regions showed comparatively high genetic diversity values compared with those from other regions in South Korea. In the unweighted pair group method with arithmetic mean (UPGMA) analysis, the 200 Perilla accessions were found to cluster into three main groups and an outgroup with 42% genetic similarity, and did not show a clear geographic structure from the five regions of South Korea. Therefore, it is believed that landrace Perilla seeds are frequently exchanged by farmers through various routes between the five regions of South Korea. The results of this study are expected to provide interesting information on the conservation of these genetic resources and selection of useful resources for the development of varieties for seeds and leafy vegetables of cultivated Perilla frutescens var. frutescens in South Korea. View Full-Text
Keywords: Perilla crop; genetic resources; morphological traits; principal component analysis; SSR marker; genetic variation Perilla crop; genetic resources; morphological traits; principal component analysis; SSR marker; genetic variation
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MDPI and ACS Style

Oh, J.S.; Sa, K.J.; Park, H.; Hyun, D.Y.; Lee, S.; Rhee, J.H.; Lee, J.K. Genetic Variation of Native Perilla Germplasms Collected from South Korea Using Simple Sequence Repeat (SSR) Markers and Morphological Characteristics. Plants 2021, 10, 1764. https://doi.org/10.3390/plants10091764

AMA Style

Oh JS, Sa KJ, Park H, Hyun DY, Lee S, Rhee JH, Lee JK. Genetic Variation of Native Perilla Germplasms Collected from South Korea Using Simple Sequence Repeat (SSR) Markers and Morphological Characteristics. Plants. 2021; 10(9):1764. https://doi.org/10.3390/plants10091764

Chicago/Turabian Style

Oh, Jun Seok, Kyu Jin Sa, Hyeon Park, Do Yoon Hyun, Sookyeong Lee, Ju Hee Rhee, and Ju Kyong Lee. 2021. "Genetic Variation of Native Perilla Germplasms Collected from South Korea Using Simple Sequence Repeat (SSR) Markers and Morphological Characteristics" Plants 10, no. 9: 1764. https://doi.org/10.3390/plants10091764

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