Dosage-Dependent Gynoecium Development and Gene Expression in Brassica napus-Orychophragmus violaceus Addition Lines
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials
2.2. Cytological Observation and GISH Analysis
2.3. DNA Extraction and Probe Labelling
2.4. RNA Extraction, cDNA Libraries Preparation, and Sequencing
2.5. Full-Length Transcriptome Analysis
2.6. Reads Mapping and Differentially Expressed Genes (DEGs) Analysis
2.7. Quantitative Real-Time PCR (qRT-PCR) Analysis
3. Results
3.1. Complete Female Sterility in Different Brassica Species Caused by Single O. violaceus Chromosome
3.2. Dosage Effect of Alien Chromosome on Gynoecium Development
3.3. RNA-seq Analysis of O. violaceus and Additions
3.4. Gene Expression Changes in Additions Induced by the O. violaceus Chromosome
3.5. DEGs Related to the Pistil Development
3.6. Down-Regulation Genes for Brassinosteroid Biosynthesis in MA
4. Discussion
4.1. Genetic Effects of Alien Chromosomes in Different Brassica Species and Dosage Effect of Alien Chromosome on Gynoecium Development
4.2. Global Gene Expression Changes upon the Alien Chromosome and Genome Ploidy
4.3. Gene Activation and Silencing Related to Female Sterility in Additions
4.4. Dosage-Dependent Gene Expression for BR Biosynthesis and Pistil Growth
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Cai, B.; Wang, T.; Fu, W.; Harun, A.; Ge, X.; Li, Z. Dosage-Dependent Gynoecium Development and Gene Expression in Brassica napus-Orychophragmus violaceus Addition Lines. Plants 2021, 10, 1766. https://doi.org/10.3390/plants10091766
Cai B, Wang T, Fu W, Harun A, Ge X, Li Z. Dosage-Dependent Gynoecium Development and Gene Expression in Brassica napus-Orychophragmus violaceus Addition Lines. Plants. 2021; 10(9):1766. https://doi.org/10.3390/plants10091766
Chicago/Turabian StyleCai, Bowei, Tai Wang, Wenqin Fu, Arrashid Harun, Xianhong Ge, and Zaiyun Li. 2021. "Dosage-Dependent Gynoecium Development and Gene Expression in Brassica napus-Orychophragmus violaceus Addition Lines" Plants 10, no. 9: 1766. https://doi.org/10.3390/plants10091766