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Effect of Ozone Treatment on Flavonoid Accumulation of Satsuma Mandarin (Citrus unshiu Marc.) during Ambient Storage

1
Hunan Key Lab of Fruits &Vegetables Storage, Processing, Quality and Safety, Hunan Agricultural Product Processing Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China
2
South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China
3
Longping branch, Graduate School of Hunan University, Changsha 410125, China
*
Authors to whom correspondence should be addressed.
Biomolecules 2019, 9(12), 821; https://doi.org/10.3390/biom9120821
Received: 11 November 2019 / Revised: 30 November 2019 / Accepted: 1 December 2019 / Published: 3 December 2019
This study aimed to compare the flavonoid accumulation between ozone-treated and untreated Satsuma mandarin (Citrus unshiu Marc.) fruits. The fruits exposed to gaseous ozone were found to have higher antioxidant activities and content of flavonoid during the storage period by ultra-high performance liquid chromatography (UPLC). To reveal the molecular regulation of flavonoid accumulation by ozone, chalcone synthase (CHS), chalcone isomerase (CHI), β-1,3-glucanase (GLU), chitinase (CHT), phenylalanine ammonia-lyase (PAL), and peroxidase (POD) were identified and their expression was examined by quantitative real-time polymerase chain reaction (q-PCR). These results support the promising application of ozone treatment as a safe food preservation technique for controlling postharvest disease and extending shelf-life of harvested Satsuma mandarin.
Keywords: satsuma mandarin; ozonation; flavonoids accumulation; antioxidant capacity; gene expression satsuma mandarin; ozonation; flavonoids accumulation; antioxidant capacity; gene expression
MDPI and ACS Style

Zhu, X.; Jiang, J.; Yin, C.; Li, G.; Jiang, Y.; Shan, Y. Effect of Ozone Treatment on Flavonoid Accumulation of Satsuma Mandarin (Citrus unshiu Marc.) during Ambient Storage. Biomolecules 2019, 9, 821.

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