Functionalized Upconversion Nanoparticles for Targeted Labelling of Bladder Cancer Cells
by
Dmitry Polikarpov 1,2, Liuen Liang 1,2, Andrew Care 2, Anwar Sunna 2, Douglas Campbell 3, 
Bradley Walsh 3, Irina Balalaeva 4, Andrei Zvyagin 2,4,5, David Gillatt 1 and Evgenii Guryev 4,*
Dmitry Polikarpov 1,2, Liuen Liang 1,2, Andrew Care 2, Anwar Sunna 2, Douglas Campbell 3, Bradley Walsh 3, Irina Balalaeva 4, Andrei Zvyagin 2,4,5, David Gillatt 1 and Evgenii Guryev 4,*
1
Faculty of Medicine and Health Sciences, Macquarie University, Sydney, NSW 2109, Australia
2
Centre for Nanoscale Biophotonics, Macquarie University, Sydney, NSW 2109, Australia
3
Glytherix Ltd., Sydney, NSW 2113, Australia
4
The Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, 23 Gagarin ave., 603950 Nizhny Novgorod, Russia
5
The Institute of Molecular Medicine, I.M. Sechenov First Moscow State Medical University, 8-2 Trubetskaya str., 119991 Moscow, Russia
*
Author to whom correspondence should be addressed.
Biomolecules 2019, 9(12), 820; https://doi.org/10.3390/biom9120820
Received: 14 November 2019 / Revised: 29 November 2019 / Accepted: 2 December 2019 / Published: 3 December 2019
Bladder cancer is the ninth most common cancer worldwide. Due to a high risk of recurrence and progression of bladder cancer, every patient needs long-term surveillance, which includes regular cystoscopy, sometimes followed by a biopsy of suspicious lesions or resections of recurring tumours. This study addresses the development of novel biohybrid nanocomplexes representing upconversion nanoparticles (UCNP) coupled to antibodies for photoluminescent (PL) detection of bladder cancer cells. Carrying specific antibodies, these nanoconjugates selectively bind to urothelial carcinoma cells and make them visible by emitting visible PL upon excitation with deeply penetrating near-infrared light. UCNP were coated with a silica layer and linked to anti-Glypican-1 antibody MIL38 via silica-specific solid-binding peptide. Conjugates have been shown to specifically attach to urothelial carcinoma cells with high expression of Glypican-1. This result highlights the potential of produced conjugates and conjugation technology for further studies of their application in the tumour detection and fluorescence-guided resection.
Keywords:
upconversion nanoparticles; photoluminescent nanocomplexes, bladder cancer, solid-binding peptide, glypican-1, photodynamic diagnostics
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
MDPI and ACS Style
Polikarpov, D.; Liang, L.; Care, A.; Sunna, A.; Campbell, D.; Walsh, B.; Balalaeva, I.; Zvyagin, A.; Gillatt, D.; Guryev, E. Functionalized Upconversion Nanoparticles for Targeted Labelling of Bladder Cancer Cells. Biomolecules 2019, 9, 820.
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