Next Article in Journal
N-Acyl Amino Acids: Metabolism, Molecular Targets, and Role in Biological Processes
Previous Article in Journal
Effect of Ozone Treatment on Flavonoid Accumulation of Satsuma Mandarin (Citrus unshiu Marc.) during Ambient Storage
 
 
Search for Articles:
Title / Keyword
Author / Affiliation
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Biomolecules
Volume 9
Issue 12
10.3390/biom9120820
biomolecules-logo
Submit to this Journal Review for this Journal Edit a Special Issue
► Article Menu

Article Menu

share announcement question_answer thumb_up
...
textsms
...
Article

Functionalized Upconversion Nanoparticles for Targeted Labelling of Bladder Cancer Cells

by
Dmitry Polikarpov
1,2,
Liuen Liang
1,2,
Andrew Care
2,
Anwar Sunna
2,
Douglas Campbell
3,
Bradley Walsh
3,
Irina Balalaeva
4,
Andrei Zvyagin
2,4,5,
David Gillatt
1 and
Evgenii Guryev
4,*
1
Faculty of Medicine and Health Sciences, Macquarie University, Sydney, NSW 2109, Australia
2
Centre for Nanoscale Biophotonics, Macquarie University, Sydney, NSW 2109, Australia
3
Glytherix Ltd., Sydney, NSW 2113, Australia
4
The Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, 23 Gagarin ave., Nizhny Novgorod 603950, Russia
5
The Institute of Molecular Medicine, I.M. Sechenov First Moscow State Medical University, 8-2 Trubetskaya str., Moscow 119991, Russia
*
Author to whom correspondence should be addressed.
Biomolecules 2019, 9(12), 820; https://doi.org/10.3390/biom9120820
Received: 14 November 2019 / Revised: 29 November 2019 / Accepted: 2 December 2019 / Published: 3 December 2019
View Full-Text Download PDF
Browse Figures
Review Reports

Abstract

Bladder cancer is the ninth most common cancer worldwide. Due to a high risk of recurrence and progression of bladder cancer, every patient needs long-term surveillance, which includes regular cystoscopy, sometimes followed by a biopsy of suspicious lesions or resections of recurring tumours. This study addresses the development of novel biohybrid nanocomplexes representing upconversion nanoparticles (UCNP) coupled to antibodies for photoluminescent (PL) detection of bladder cancer cells. Carrying specific antibodies, these nanoconjugates selectively bind to urothelial carcinoma cells and make them visible by emitting visible PL upon excitation with deeply penetrating near-infrared light. UCNP were coated with a silica layer and linked to anti-Glypican-1 antibody MIL38 via silica-specific solid-binding peptide. Conjugates have been shown to specifically attach to urothelial carcinoma cells with high expression of Glypican-1. This result highlights the potential of produced conjugates and conjugation technology for further studies of their application in the tumour detection and fluorescence-guided resection. View Full-Text
Keywords: upconversion nanoparticles; photoluminescent nanocomplexes; bladder cancer; solid-binding peptide; glypican-1; photodynamic diagnostics upconversion nanoparticles; photoluminescent nanocomplexes; bladder cancer; solid-binding peptide; glypican-1; photodynamic diagnostics
►▼ Show Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

SciFeed

Share and Cite

MDPI and ACS Style

Polikarpov, D.; Liang, L.; Care, A.; Sunna, A.; Campbell, D.; Walsh, B.; Balalaeva, I.; Zvyagin, A.; Gillatt, D.; Guryev, E. Functionalized Upconversion Nanoparticles for Targeted Labelling of Bladder Cancer Cells. Biomolecules 2019, 9, 820. https://doi.org/10.3390/biom9120820

AMA Style

Polikarpov D, Liang L, Care A, Sunna A, Campbell D, Walsh B, Balalaeva I, Zvyagin A, Gillatt D, Guryev E. Functionalized Upconversion Nanoparticles for Targeted Labelling of Bladder Cancer Cells. Biomolecules. 2019; 9(12):820. https://doi.org/10.3390/biom9120820

Chicago/Turabian Style

Polikarpov, Dmitry, Liuen Liang, Andrew Care, Anwar Sunna, Douglas Campbell, Bradley Walsh, Irina Balalaeva, Andrei Zvyagin, David Gillatt, and Evgenii Guryev. 2019. "Functionalized Upconversion Nanoparticles for Targeted Labelling of Bladder Cancer Cells" Biomolecules 9, no. 12: 820. https://doi.org/10.3390/biom9120820

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Metrics

Article Access Map by Country/Region

1
Back to TopTop