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Article

Lineage 1 Porcine Reproductive and Respiratory Syndrome Virus Attenuated Live Vaccine Provides Broad Cross-Protection against Homologous and Heterologous NADC30-Like Virus Challenge in Piglets

State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Hiep L. X. Vu
Vaccines 2022, 10(5), 752; https://doi.org/10.3390/vaccines10050752
Received: 29 March 2022 / Revised: 19 April 2022 / Accepted: 8 May 2022 / Published: 10 May 2022
(This article belongs to the Special Issue Swine Vaccines and Vaccinology)
Porcine reproductive and respiratory syndrome virus (PRRSV) is an important pathogen that endangers the swine industry worldwide. Recently, lineage 1 PRRSVs, especially NADC30-like PRRSVs, have become the major endemic strains in many pig-breeding countries. Since 2016, NADC30-like PRRSV has become the predominant strain in China. Unfortunately, current commercial vaccines cannot provide sufficient protection against this strain. Here, an attenuated lineage 1 PRRSV strain, named SD-R, was obtained by passaging an NADC30-like PRRSV strain SD in Marc-145 cells for 125 passages. Four-week-old PRRSV-free piglets were vaccinated intramuscularly with 105.0TCID50 SD-R and then challenged intramuscularly (2 mL) and intranasally (2 mL) with homologous NADC30-like PRRSV SD (1 × 105.0TCID50/mL) and heterologous NADC30-like PRRSV HLJWK108-1711 (1 × 105.0TCID50/mL). The results showed that antibodies against specific PRRSVs in 5 of 5 immunized piglets were positive after a 14-day post-vaccination and did not develop fever or clinical diseases after NADC30-like PRRSV challenges. Additionally, compared with challenge control piglets, vaccinated piglets gained significantly more weight and showed much milder pathological lesions. Furthermore, the viral replication levels of the immunized group were significantly lower than those of the challenge control group. These results demonstrate that lineage 1 PRRSV SD-R is a good candidate for an efficacious vaccine, providing complete clinical protection for piglets against NADC30-like PRRSVs. View Full-Text
Keywords: PRRSV; lineage 1; NADC30-like; SD-R; pathogenicity; cross-protection efficacy PRRSV; lineage 1; NADC30-like; SD-R; pathogenicity; cross-protection efficacy
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MDPI and ACS Style

Zhang, H.; Xiang, L.; Xu, H.; Li, C.; Tang, Y.-D.; Gong, B.; Zhang, W.; Zhao, J.; Song, S.; Peng, J.; Wang, Q.; An, T.; Cai, X.; Tian, Z.-J. Lineage 1 Porcine Reproductive and Respiratory Syndrome Virus Attenuated Live Vaccine Provides Broad Cross-Protection against Homologous and Heterologous NADC30-Like Virus Challenge in Piglets. Vaccines 2022, 10, 752. https://doi.org/10.3390/vaccines10050752

AMA Style

Zhang H, Xiang L, Xu H, Li C, Tang Y-D, Gong B, Zhang W, Zhao J, Song S, Peng J, Wang Q, An T, Cai X, Tian Z-J. Lineage 1 Porcine Reproductive and Respiratory Syndrome Virus Attenuated Live Vaccine Provides Broad Cross-Protection against Homologous and Heterologous NADC30-Like Virus Challenge in Piglets. Vaccines. 2022; 10(5):752. https://doi.org/10.3390/vaccines10050752

Chicago/Turabian Style

Zhang, Hongliang, Lirun Xiang, Hu Xu, Chao Li, Yan-Dong Tang, Bangjun Gong, Wenli Zhang, Jing Zhao, Shuaijie Song, Jinmei Peng, Qian Wang, Tongqing An, Xuehui Cai, and Zhi-Jun Tian. 2022. "Lineage 1 Porcine Reproductive and Respiratory Syndrome Virus Attenuated Live Vaccine Provides Broad Cross-Protection against Homologous and Heterologous NADC30-Like Virus Challenge in Piglets" Vaccines 10, no. 5: 752. https://doi.org/10.3390/vaccines10050752

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