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Article

The Potential of a Hair Follicle Mesenchymal Stem Cell-Conditioned Medium for Wound Healing and Hair Follicle Regeneration

by
Keng-Liang Ou
1,2,3,4,5,
Yun-Wen Kuo
6,†,
Chia-Yu Wu
7,8,
Bai-Hung Huang
4,9,10,
Fang-Tzu Pai
4,8,
Hsin-Hua Chou
11,12,
Takashi Saito
13,
Takaaki Ueno
3,
Yung-Chieh Cho
4,11 and
Mao-Suan Huang
1,14,*
1
Department of Dentistry, Taipei Medical University-Shuang Ho Hospital, New Taipei City 235, Taiwan
2
Department of Oral Hygiene Care, Ching Kuo Institute of Management and Health, Keelung 203, Taiwan
3
Department of Dentistry and Oral Surgery, Osaka Medical College, Osaka 569-8686, Japan
4
Biomedical Technology R & D Center, China Medical University Hospital, Taichung 404, Taiwan
5
3D Global Biotech Inc. (Spin-off Company from Taipei Medical University), New Taipei City 221, Taiwan
6
Division of Prosthodontics, Department of Dentistry, Taipei Medical University Hospital, Taipei 110, Taiwan
7
Division of Oral and Maxillofacial Surgery, Department of Dentistry, Taipei Medical University Hospital, Taipei 110, Taiwan
8
School of Dental Technology, College of Oral Medicine, Taipei Medical University, Taipei 110, Taiwan
9
Asia Pacific Laser Institute, New Taipei City 220, Taiwan
10
Implant Academy of Minimally Invasive Dentistry, Taipei 106, Taiwan
11
School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 110, Taiwan
12
Dental Department of Wan-Fang Hospital, Taipei Medical University, Taipei 116, Taiwan
13
Division of Clinical Cariology and Endodontology, Department of Oral Rehabilitation, School of Dentistry, Health Sciences University of Hokkaido, Hokkaido 061-0293, Japan
14
School of Oral Hygiene, College of Oral Medicine, Taipei Medical University, Taipei 110, Taiwan
 
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*
Author to whom correspondence should be addressed.
†
Co-first author: Yun-Wen Kuo.
Appl. Sci. 2020, 10(8), 2646; https://doi.org/10.3390/app10082646
Submission received: 11 March 2020 / Revised: 8 April 2020 / Accepted: 9 April 2020 / Published: 11 April 2020
(This article belongs to the Special Issue Application of the Biocomposite Materials on Bone Reconstruction)
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Abstract

The study elucidated the wound healing and hair regeneration properties of a conditioned medium prepared from the culture of human hair follicle mesenchymal stem cells (HFMSCs). The wound-healing effects of mesenchymal stem cell-conditioned medium (MSC-CM) were tested in vitro using scratch assays co-cultured with HaCaT keratinocyte and monitored through optical microscopy. The cell proliferation of HFMSCs and the HaCaT keratinocyte were observed in the presence of different kinds of drugs including UK5099, sodium L-lactate, lactate dehydrogenase-A, MSC-CM, caffeine, and caffeic acid. The hair regeneration properties were investigated in vivo by administrating the MSC-CM solutions to adult B6 mouse models. For quantification, hematoxylin and eosin staining were performed following euthanasia. In vitro results revealed that MSC-CM promotes dermal cell migrations and enhances proliferation of HFMSCs and HaCaT keratinocytes, demonstrating wound-healing properties. Moreover, when the MSC-CM solutions were applied to the shaved mouse skin, a dark area that expanded overtime was seen. Although no hair growth was found, histological analysis proved that a fat layer thickness increment was found under the mouse’s skin, ultimately projecting the formation of new hair growth. MSC-CM promotes the migration and proliferation of dermal keratinocytes that are beneficial for wound healing and hair growth. It is believed that MSC-CM can potentially serve as the basis of alternative therapeutic applications for wound closure and skin regeneration as well as hair growth stimulation and hair loss prevention in alopecia.
Keywords: hair follicle; hair regeneration; mesenchymal stem cells; wound healing hair follicle; hair regeneration; mesenchymal stem cells; wound healing

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MDPI and ACS Style

Ou, K.-L.; Kuo, Y.-W.; Wu, C.-Y.; Huang, B.-H.; Pai, F.-T.; Chou, H.-H.; Saito, T.; Ueno, T.; Cho, Y.-C.; Huang, M.-S. The Potential of a Hair Follicle Mesenchymal Stem Cell-Conditioned Medium for Wound Healing and Hair Follicle Regeneration. Appl. Sci. 2020, 10, 2646. https://doi.org/10.3390/app10082646

AMA Style

Ou K-L, Kuo Y-W, Wu C-Y, Huang B-H, Pai F-T, Chou H-H, Saito T, Ueno T, Cho Y-C, Huang M-S. The Potential of a Hair Follicle Mesenchymal Stem Cell-Conditioned Medium for Wound Healing and Hair Follicle Regeneration. Applied Sciences. 2020; 10(8):2646. https://doi.org/10.3390/app10082646

Chicago/Turabian Style

Ou, Keng-Liang, Yun-Wen Kuo, Chia-Yu Wu, Bai-Hung Huang, Fang-Tzu Pai, Hsin-Hua Chou, Takashi Saito, Takaaki Ueno, Yung-Chieh Cho, and Mao-Suan Huang. 2020. "The Potential of a Hair Follicle Mesenchymal Stem Cell-Conditioned Medium for Wound Healing and Hair Follicle Regeneration" Applied Sciences 10, no. 8: 2646. https://doi.org/10.3390/app10082646

APA Style

Ou, K.-L., Kuo, Y.-W., Wu, C.-Y., Huang, B.-H., Pai, F.-T., Chou, H.-H., Saito, T., Ueno, T., Cho, Y.-C., & Huang, M.-S. (2020). The Potential of a Hair Follicle Mesenchymal Stem Cell-Conditioned Medium for Wound Healing and Hair Follicle Regeneration. Applied Sciences, 10(8), 2646. https://doi.org/10.3390/app10082646

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