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Article

Transcriptome Sequencing and Comparative Analysis of Amphoteric ESCs and PGCs in Chicken (Gallus gallus)

by 1,2,3, 1,2,3, 1,2,3, 4, 1,2,3, 1,2,3, 1,2,3 and 1,2,3,*
1
Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
2
Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou 225009, China
3
Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou 225009, China
4
Animal & Avian Sciences, University of Maryland, College Park, MD 20741, USA
*
Author to whom correspondence should be addressed.
Animals 2020, 10(12), 2228; https://doi.org/10.3390/ani10122228
Received: 19 October 2020 / Revised: 24 November 2020 / Accepted: 26 November 2020 / Published: 27 November 2020
(This article belongs to the Collection Genetic Diversity in Livestock and Companion Animals)
The study of chicken embryonic stem cells (ESCs) and primordial germ cells (PGCs) showed the potential application of developmental biology and translational medicine. However, the difference between amphoteric ESCs and PGCs is still elusive, limiting the accuracy of correlative research. In this paper, chicken amphoteric ESCs and PGCs were isolated, separated, and sequenced to explore their dynamic transcriptomes. Our results provide a knowledge base of transcriptomes in chicken amphoteric ESCs and PGCs, which will help other researchers interested in studying relative biological processes.
Chicken (Gallus gallus) pluripotent embryonic stem cells (ESCs) and primordial germ cells (PGCs) can be broadly applied in the research of developmental and embryonic biology, but the difference between amphoteric ESCs and PGCs is still elusive. This study determined the sex of collected samples by identifying specific sex markers via polymerase chain reaction (PCR) and fluorescence activated cell sorter (FACS). RNA-seq was utilized to investigate the transcriptomic profile of amphoteric ESCs and PGCs in chicken. The results showed no significant differentially expressed genes (DEGs) in amphoteric ESCs and 227 DEGs exhibited in amphoteric PGCs. Moreover, those 227 DEGs were mainly enriched in 17 gene ontology (GO) terms and 27 pathways according to Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Furthermore, qRT-PCR was performed to verify RNA-seq results, and the results demonstrated that Notch1 was highly expressed in male PGCs. In summary, our results provided a knowledge base of chicken amphoteric ESCs and PGCs, which is helpful for future research in relevant biological processes. View Full-Text
Keywords: differentially expressed genes; transcriptome analysis; amphoteric ESCs; amphoteric PGCs; chicken (Gallus gallus) differentially expressed genes; transcriptome analysis; amphoteric ESCs; amphoteric PGCs; chicken (Gallus gallus)
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MDPI and ACS Style

Jin, K.; Zhou, J.; Zuo, Q.; Song, J.; Zhang, Y.; Chang, G.; Chen, G.; Li, B. Transcriptome Sequencing and Comparative Analysis of Amphoteric ESCs and PGCs in Chicken (Gallus gallus). Animals 2020, 10, 2228. https://doi.org/10.3390/ani10122228

AMA Style

Jin K, Zhou J, Zuo Q, Song J, Zhang Y, Chang G, Chen G, Li B. Transcriptome Sequencing and Comparative Analysis of Amphoteric ESCs and PGCs in Chicken (Gallus gallus). Animals. 2020; 10(12):2228. https://doi.org/10.3390/ani10122228

Chicago/Turabian Style

Jin, Kai, Jing Zhou, Qisheng Zuo, Jiuzhou Song, Yani Zhang, Guobing Chang, Guohong Chen, and Bichun Li. 2020. "Transcriptome Sequencing and Comparative Analysis of Amphoteric ESCs and PGCs in Chicken (Gallus gallus)" Animals 10, no. 12: 2228. https://doi.org/10.3390/ani10122228

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