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Increase of Glycoalkaloid Content in Potato Tubers by Greening as a Method to Reduce the Spread of Pectobacterium and Dickeya spp. in Seed Production Systems
 
 
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Microorganisms
Volume 11
Issue 3
10.3390/microorganisms11030620
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Article

Field Use of Protective Bacteriophages against Pectinolytic Bacteria of Potato

by
Karel Petrzik
1,
Josef Vacek
2,
Martin Kmoch
3,*,
Denisa Binderová
3,
Sára Brázdová
1,
Ondřej Lenz
1 and
Rudolf Ševčík
4
1
Institute of Molecular Biology, Biology Centre of the Czech Academy of Sciences, Branišovská 1160/31, 370 05 České Budějovice, Czech Republic
2
Department of Growing Technologies, Potato Research Institute Havlíčkův Brod, Dobrovského 2366, 580 01 Havlíčkův Brod, Czech Republic
3
Laboratory of Virology, Department of Genetic Resources, Potato Research Institute Havlíčkův Brod, Dobrovského 2366, 580 01 Havlíčkův Brod, Czech Republic
4
Institute of Food Preservation, Faculty of Food and Biochemical Technology, University of Chemistry and Technology (VŠCHT), Technická 3, 166 28 Prague, Czech Republic
*
Author to whom correspondence should be addressed.
Microorganisms 2023, 11(3), 620; https://doi.org/10.3390/microorganisms11030620
Submission received: 9 February 2023 / Revised: 24 February 2023 / Accepted: 25 February 2023 / Published: 28 February 2023
(This article belongs to the Special Issue Dickeya and Pectobacterium: Ecology, Pathology and Plant Protection 2.0)
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Abstract

The pectinolytic Dickeya solani bacterium is an important pathogen found in potatoes. We conducted laboratory and field experiments mimicking severe and mild Dickeya spp. infection and investigated the application of a mixture of two lytic bacteriophages before and after bacterial infection to protect the plants. Application of the phage solution to tuber disks and wounded tubers did not completely eliminate the infection but reduced the development of soft rot symptoms by 59.5–91.4%, depending on the phage concentration. In the field trial, plants treated with bacteriophages after severe Dickeya infection had 5–33% greater leaf cover and 4–16% greater tuber yield compared to untreated plants. When simulating a mild infection, leaf cover was 11–42% greater, and tuber yield was 25–31% greater compared to untreated plants. We conclude that the phage mixture has the potential to protect potatoes ecologically from D. solani.
Keywords: Enterobacteriaceae; Solanum tuberosum; Limestonevirus; biological control Enterobacteriaceae; Solanum tuberosum; Limestonevirus; biological control

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MDPI and ACS Style

Petrzik, K.; Vacek, J.; Kmoch, M.; Binderová, D.; Brázdová, S.; Lenz, O.; Ševčík, R. Field Use of Protective Bacteriophages against Pectinolytic Bacteria of Potato. Microorganisms 2023, 11, 620. https://doi.org/10.3390/microorganisms11030620

AMA Style

Petrzik K, Vacek J, Kmoch M, Binderová D, Brázdová S, Lenz O, Ševčík R. Field Use of Protective Bacteriophages against Pectinolytic Bacteria of Potato. Microorganisms. 2023; 11(3):620. https://doi.org/10.3390/microorganisms11030620

Chicago/Turabian Style

Petrzik, Karel, Josef Vacek, Martin Kmoch, Denisa Binderová, Sára Brázdová, Ondřej Lenz, and Rudolf Ševčík. 2023. "Field Use of Protective Bacteriophages against Pectinolytic Bacteria of Potato" Microorganisms 11, no. 3: 620. https://doi.org/10.3390/microorganisms11030620

APA Style

Petrzik, K., Vacek, J., Kmoch, M., Binderová, D., Brázdová, S., Lenz, O., & Ševčík, R. (2023). Field Use of Protective Bacteriophages against Pectinolytic Bacteria of Potato. Microorganisms, 11(3), 620. https://doi.org/10.3390/microorganisms11030620

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