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Insects
Volume 11
Issue 8
10.3390/insects11080497
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Article

Bacterial Communities in Three Parts of Intestinal Tracts of Carpenter Bees (Xylocopa tenuiscapa)

by
Phakamas Subta
1,
Phongsathon Yodsuwan
1,
Rujipas Yongsawas
1,
Ammarin In-on
2,
Natapot Warrit
3,
Somsak Panha
4,
Kitiphong Khongphinitbunjong
5,
Panuwan Chantawannakul
1,
Korrawat Attasopa
6 and
Terd Disayathanoowat
1,7,8,*
1
Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand
2
Bioinformatics & Systems Biology Program, King Mongkut’s University of Technology Thonburi (Bang Khun Thian Campus), Bang Khun Thian, Bangkok 10150, Thailand
3
Center of Excellence in Entomology, Department of Biology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand
4
Department of Biology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand
5
School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand
6
Department of Entomology and Plant Pathology, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand
7
Research Center in Bioresources for Agriculture, Industry and Medicine, Chiang Mai University, Chiang Mai 50200, Thailand
8
Research Center of Microbial Diversity and Sustainable Utilization, Chiang Mai University, Chiang Mai 50200, Thailand
*
Author to whom correspondence should be addressed.
Insects 2020, 11(8), 497; https://doi.org/10.3390/insects11080497
Submission received: 1 July 2020 / Revised: 27 July 2020 / Accepted: 30 July 2020 / Published: 3 August 2020
(This article belongs to the Collection Bees and Their Symbionts)
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Abstract

This study investigated different bacterial communities in three intestinal parts (foregut, midgut and hindgut) of Xylocopatenuiscapa to understand the roles of gut bacteria. Our phylogenetic analysis revealed that X. tenuiscapa is closely related to Xylocopa latipes. The 16S rRNA gene in the genomic DNA samples from the gut was examined by illumina (Solexa) and a total of 998 operational taxonomic unit (OTUs) clusters were found. Taxonomic classification identified 16 bacterial phyla and unclassified bacteria. The dominant bacteria taxa in the three parts of X. tenuiscapa gut were Proteobacteria, Firmicutes, Bacteroidetes and Actinobacteria. In the foregut, Lactobacillales and Enterobacteriaceae were predominantly found. The population in the midgut was similar to that in the foregut, with the addition of Gilliamella, which was also abundant. The most dominant bacteria identified in the hindgut were similar to those in the midgut and Lactobacillales, Enterobacteriaceae, Gilliamella, Bifidobacteriaceae and Flavobacteriaceae appeared in abundance. Moreover, our results suggest that a community structure of bacteria in different parts of X. tenuiscapa’s gut may be an important indicator of carpenter bees’ health. This functional study of bacterial communities revealed significant differences among the three intestinal parts and is the first report of the gut bacteria structure in solitary bees.
Keywords: carpenter bees; intestine; microbiota; next-generation sequencing carpenter bees; intestine; microbiota; next-generation sequencing

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MDPI and ACS Style

Subta, P.; Yodsuwan, P.; Yongsawas, R.; In-on, A.; Warrit, N.; Panha, S.; Khongphinitbunjong, K.; Chantawannakul, P.; Attasopa, K.; Disayathanoowat, T. Bacterial Communities in Three Parts of Intestinal Tracts of Carpenter Bees (Xylocopa tenuiscapa). Insects 2020, 11, 497. https://doi.org/10.3390/insects11080497

AMA Style

Subta P, Yodsuwan P, Yongsawas R, In-on A, Warrit N, Panha S, Khongphinitbunjong K, Chantawannakul P, Attasopa K, Disayathanoowat T. Bacterial Communities in Three Parts of Intestinal Tracts of Carpenter Bees (Xylocopa tenuiscapa). Insects. 2020; 11(8):497. https://doi.org/10.3390/insects11080497

Chicago/Turabian Style

Subta, Phakamas, Phongsathon Yodsuwan, Rujipas Yongsawas, Ammarin In-on, Natapot Warrit, Somsak Panha, Kitiphong Khongphinitbunjong, Panuwan Chantawannakul, Korrawat Attasopa, and Terd Disayathanoowat. 2020. "Bacterial Communities in Three Parts of Intestinal Tracts of Carpenter Bees (Xylocopa tenuiscapa)" Insects 11, no. 8: 497. https://doi.org/10.3390/insects11080497

APA Style

Subta, P., Yodsuwan, P., Yongsawas, R., In-on, A., Warrit, N., Panha, S., Khongphinitbunjong, K., Chantawannakul, P., Attasopa, K., & Disayathanoowat, T. (2020). Bacterial Communities in Three Parts of Intestinal Tracts of Carpenter Bees (Xylocopa tenuiscapa). Insects, 11(8), 497. https://doi.org/10.3390/insects11080497

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