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Pharmaceutics
Volume 15
Issue 6
10.3390/pharmaceutics15061763
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Article

Evaluation of the In Vitro Antimicrobial Efficacy against Staphylococcus aureus and epidermidis of a Novel 3D-Printed Degradable Drug Delivery System Based on Polycaprolactone/Chitosan/Vancomycin—Preclinical Study

by
Iván López-González
1,
Ana Belén Hernández-Heredia
2,
María Isabel Rodríguez-López
2,
David Auñón-Calles
2,
Mohamed Boudifa
3,
José Antonio Gabaldón
2,* and
Luis Meseguer-Olmo
1,*
1
Tissue Regeneration and Repair Group: Orthobiology, Biomaterials and Tissue Engineering, UCAM—Universidad Católica de Murcia, Campus de los Jerónimos 135, Guadalupe, 30107 Murcia, Spain
2
Molecular Recognition and Encapsulation Research Group (REM), Health Sciences Department, UCAM—Universidad Católica de Murcia, Campus de los Jerónimos 135, Guadalupe, 30107 Murcia, Spain
3
CRITT—Matériaux Innovation, 9 Rue Claude Chrétien, Campus Sup Ardenne, 08000 Charleville-Mézières, France
*
Authors to whom correspondence should be addressed.
Pharmaceutics 2023, 15(6), 1763; https://doi.org/10.3390/pharmaceutics15061763
Submission received: 6 May 2023 / Revised: 10 June 2023 / Accepted: 14 June 2023 / Published: 18 June 2023
(This article belongs to the Special Issue Polymers Enhancing Bioavailability in Drug Delivery, 2nd Edition)
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Abstract

Acute and chronic bone infections, especially those caused by methicillin-resistant Staphylococcus aureus (MRSA), remains a major complication and therapeutic challenge. It is documented that local administration of vancomycin offers better results than the usual routes of administration (e.g., intravenous) when ischemic areas are present. In this work, we evaluate the antimicrobial efficacy against S. aureus and S. epidermidis of a novel hybrid 3D-printed scaffold based on polycaprolactone (PCL) and a chitosan (CS) hydrogel loaded with different vancomycin (Van) concentrations (1, 5, 10, 20%). Two cold plasma treatments were used to improve the adhesion of CS hydrogels to the PCL scaffolds by decreasing PCL hydrophobicity. Vancomycin release was measured by means of HPLC, and the biological response of ah-BM-MSCs growing in the presence of the scaffolds was evaluated in terms of cytotoxicity, proliferation, and osteogenic differentiation. The PCL/CS/Van scaffolds tested were found to be biocompatible, bioactive, and bactericide, as demonstrated by no cytotoxicity (LDH activity) or functional alteration (ALP activity, alizarin red staining) of the cultured cells and by bacterial inhibition. Our results suggest that the scaffolds developed would be excellent candidates for use in a wide range of biomedical fields such as drug delivery systems or tissue engineering applications.
Keywords: 3D printing; hybrid scaffold; polycaprolactone; chitosan; vancomycin; mesenchymal stem cells; tissue engineering; drug delivery systems (DDSs); osteomyelitis 3D printing; hybrid scaffold; polycaprolactone; chitosan; vancomycin; mesenchymal stem cells; tissue engineering; drug delivery systems (DDSs); osteomyelitis

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MDPI and ACS Style

López-González, I.; Hernández-Heredia, A.B.; Rodríguez-López, M.I.; Auñón-Calles, D.; Boudifa, M.; Gabaldón, J.A.; Meseguer-Olmo, L. Evaluation of the In Vitro Antimicrobial Efficacy against Staphylococcus aureus and epidermidis of a Novel 3D-Printed Degradable Drug Delivery System Based on Polycaprolactone/Chitosan/Vancomycin—Preclinical Study. Pharmaceutics 2023, 15, 1763. https://doi.org/10.3390/pharmaceutics15061763

AMA Style

López-González I, Hernández-Heredia AB, Rodríguez-López MI, Auñón-Calles D, Boudifa M, Gabaldón JA, Meseguer-Olmo L. Evaluation of the In Vitro Antimicrobial Efficacy against Staphylococcus aureus and epidermidis of a Novel 3D-Printed Degradable Drug Delivery System Based on Polycaprolactone/Chitosan/Vancomycin—Preclinical Study. Pharmaceutics. 2023; 15(6):1763. https://doi.org/10.3390/pharmaceutics15061763

Chicago/Turabian Style

López-González, Iván, Ana Belén Hernández-Heredia, María Isabel Rodríguez-López, David Auñón-Calles, Mohamed Boudifa, José Antonio Gabaldón, and Luis Meseguer-Olmo. 2023. "Evaluation of the In Vitro Antimicrobial Efficacy against Staphylococcus aureus and epidermidis of a Novel 3D-Printed Degradable Drug Delivery System Based on Polycaprolactone/Chitosan/Vancomycin—Preclinical Study" Pharmaceutics 15, no. 6: 1763. https://doi.org/10.3390/pharmaceutics15061763

APA Style

López-González, I., Hernández-Heredia, A. B., Rodríguez-López, M. I., Auñón-Calles, D., Boudifa, M., Gabaldón, J. A., & Meseguer-Olmo, L. (2023). Evaluation of the In Vitro Antimicrobial Efficacy against Staphylococcus aureus and epidermidis of a Novel 3D-Printed Degradable Drug Delivery System Based on Polycaprolactone/Chitosan/Vancomycin—Preclinical Study. Pharmaceutics, 15(6), 1763. https://doi.org/10.3390/pharmaceutics15061763

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