Table of Contents
Viruses, Volume 10, Issue 12 (December 2018) – 71 articles
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Cover Story (view full-size image) We employed two different approaches to mutate Sulfolobus islandicus rod-shaped virus 2 (SIRV2). [...] Read more. We employed two different approaches to mutate Sulfolobus islandicus rod-shaped virus 2 (SIRV2). The anti-CRISPR (Acr) gene acrID1 was used as a selection marker to knock out genes from an acrID1-null mutant of SIRV2. Moreover, the endogenous CRISPR-Cas systems of its host were repurposed to knock out all the accessory genes. The results are relevant for future functional studies, and such approaches are applicable to other virus–host systems. View Paper here.