Next Article in Journal
Different Outcomes of Experimental Hepatitis E Virus Infection in Diverse Mouse Strains, Wistar Rats, and Rabbits
Next Article in Special Issue
Viral Delivery Systems for CRISPR
Previous Article in Journal
Global Interactomics Connect Nuclear Mitotic Apparatus Protein NUMA1 to Influenza Virus Maturation
Previous Article in Special Issue
Anti-CRISPR-Based and CRISPR-Based Genome Editing of Sulfolobus islandicus Rod-Shaped Virus 2
Open AccessArticle

Engineering RNA Virus Interference via the CRISPR/Cas13 Machinery in Arabidopsis

Laboratory for Genome Engineering, Division of Biological Sciences, 4700 King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia
*
Author to whom correspondence should be addressed.
Viruses 2018, 10(12), 732; https://doi.org/10.3390/v10120732
Received: 8 November 2018 / Revised: 9 December 2018 / Accepted: 18 December 2018 / Published: 19 December 2018
(This article belongs to the Special Issue Applications of CRISPR Technology in Virology 2018)
Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) systems are key immune mechanisms helping prokaryotic species fend off RNA and DNA viruses. CRISPR/Cas9 has broad applications in basic research and biotechnology and has been widely used across eukaryotic species for genome engineering and functional analysis of genes. The recently developed CRISPR/Cas13 systems target RNA rather than DNA and thus offer new potential for transcriptome engineering and combatting RNA viruses. Here, we used CRISPR/LshCas13a to stably engineer Arabidopsis thaliana for interference against the RNA genome of Turnip mosaic virus (TuMV). Our data demonstrate that CRISPR RNAs (crRNAs) guiding Cas13a to the sequences encoding helper component proteinase silencing suppressor (HC-Pro) or GFP target 2 (GFP-T2) provide better interference compared to crRNAs targeting other regions of the TuMV RNA genome. This work demonstrates the exciting potential of CRISPR/Cas13 to be used as an antiviral strategy to obstruct RNA viruses, and encourages the search for more robust and effective Cas13 variants or CRISPR systems that can target RNA. View Full-Text
Keywords: CRISPR/Cas systems; CRISPR/Cas13a; virus interference; RNA interference; molecular immunity; RNA knockdown; transcriptome regulation CRISPR/Cas systems; CRISPR/Cas13a; virus interference; RNA interference; molecular immunity; RNA knockdown; transcriptome regulation
Show Figures

Figure 1

MDPI and ACS Style

Aman, R.; Mahas, A.; Butt, H.; Ali, Z.; Aljedaani, F.; Mahfouz, M. Engineering RNA Virus Interference via the CRISPR/Cas13 Machinery in Arabidopsis. Viruses 2018, 10, 732.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop