Seasonal droughts induced by climate change pose a significant threat to the normal growth patterns of forests in the subtropical regions of southern China. Therefore, it is crucial to explore the response of tree water use patterns to seasonal drought to maintain tree physiological activities. However, it remains unknown whether changes in dry and wet seasons have an impact on the water use patterns of trees of different ages. In this study, a two-year experiment was conducted in
Eucalyptus urophylla × Eucalyptus grandis (hereinafter referred to as
Eucalyptus) plantations at three ages (4, 7, and 17 years). Specifically, the water use patterns of
Eucalyptus in dry and wet seasons were calculated using hydrogen stable isotopes (including the isotopes in xylem water and 0–150 cm soil layers) coupled with MixSIAR. The results showed that there were notable variations in the proportions of water absorption from different soil layers by
Eucalyptus during dry and wet seasons. During the dry season (April 2024), 4-year-old and 7-year-old
Eucalyptus primarily utilized water from the 40–90 cm soil layer, while 17-year-old
Eucalyptus mainly relied on deep soil water at depths of 60–150 cm, with a utilization ratio of 50.9%. During the wet season (August 2023), the depth of water uptake by
Eucalyptus of different ages significantly shifted towards shallow layers, and the trees primarily utilized surface soil water from the 0–60 cm layer, with utilization ratios of 59.9%, 64.8%, and 61.6% for 4-year-old, 7-year-old, and 17-year-old
Eucalyptus, respectively. The water sources of
Eucalyptus during dry and wet seasons were variable, which allowed
Eucalyptus to cope with seasonal drought stress. The differences in the water uptake strategies of
Eucalyptus between dry and wet seasons can be attributed to their long-term adaptation to the environment. Our research revealed the differences in the water utilization of
Eucalyptus with various ages between dry and wet seasons in subtropical China, providing new insights for a better understanding of the adaptive mechanisms of subtropical forests in response to alterations in water conditions caused by climate change.
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