Sensors, Volume 23, Issue 17
September-1 2023 - 354 articles
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Cover Story: A bio-functionalized solution-immersed silicon (SIS) sensor was developed at the single-cell level to identify Erwinia amylovora (E. amylovora). Lipopolysaccharide Transporter E (LptE), which is involved in the assembly of lipopolysaccharide (LPS) at the surface of the outer membrane of E. amylovora, was employed as a capture agent to detect a single cell of E. amylovora. It was confirmed that LptE interacts with E. amylovora via LPS using in-house ELISA analysis; it was then used to construct the sensor chip by immobilizing the capture molecule on the sensor surface modified with 3′-Aminopropyl triethoxysilane (APTES) and glutaraldehyde (GA). The LptE-based SIS sensor exhibited the sensitive and specific detection of the target bacterial cell in real time. View this paper