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Volume 21
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10.3390/ijms21051618
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Article

Evaluation of the Genotoxic and Oxidative Damage Potential of Silver Nanoparticles in Human NCM460 and HCT116 Cells

by
Mingxi Jia
1,2,†,
Wenjing Zhang
1,
Taojin He
1,
Meng Shu
1,
Jing Deng
1,2,†,
Jianhui Wang
3,†,
Wen Li
1,2,*,
Jie Bai
1,*,
Qinlu Lin
1,
Feijun Luo
1,
Wenhua Zhou
1 and
Xiaoxi Zeng
2
1
Hunan Key Laboratory of Processed Food for Special Medical Purpose, Hunan Key Laboratory of Grain-Oil Deep Process and Quality Control, College of Food Science and Engineering, National Engineering Laboratory for Deep Process of Rice and Byproducts, Central South University of Forestry and Technology, Changsha 410004, China
2
Key Laboratory of Biological Nanomaterials and Devices, College of life sciences and chemistry, Hunan University of Technology, Zhuzhou 412007, China
3
School of Chemistry and Food Engineering, Changsha University of Science and Technology, Changsha 410114, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(5), 1618; https://doi.org/10.3390/ijms21051618
Received: 14 January 2020 / Revised: 23 February 2020 / Accepted: 24 February 2020 / Published: 27 February 2020
(This article belongs to the Special Issue Nanotoxicology and Nanosafety 2.0)
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Abstract

Nano Ag has excellent antibacterial properties and is widely used in various antibacterial materials, such as antibacterial medicine and medical devices, food packaging materials and antibacterial textiles. Despite the many benefits of nano-Ag, more and more research indicates that it may have potential biotoxic effects. Studies have shown that people who ingest nanoparticles by mouth have the highest uptake in the intestinal tract, and that the colon area is the most vulnerable to damage and causes the disease. In this study, we examined the toxic effects of different concentrations of Ag-NPs on normal human colon cells (NCM460) and human colon cancer cells (HCT116). As the concentration of nanoparticles increased, the activity of the two colon cells decreased and intracellular reactive oxygen species (ROS) increased. RT-qPCR and Western-blot analyses showed that Ag NPs can promote the increase in P38 protein phosphorylation levels in two colon cells and promote the expression of P53 and Bax. The analysis also showed that Ag NPs can promote the down-regulation of Bcl-2, leading to an increased Bax/Bcl-2 ratio and activation of P21, further accelerating cell death. This study showed that a low concentration of nano Ag has no obvious toxic effect on colon cells, while nano Ag with concentrations higher than 15 μg/mL will cause oxidative damage to colon cells. View Full-Text
Keywords: nano-Ag; colon cells; biological toxicity; oxidative damage nano-Ag; colon cells; biological toxicity; oxidative damage
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MDPI and ACS Style

Jia, M.; Zhang, W.; He, T.; Shu, M.; Deng, J.; Wang, J.; Li, W.; Bai, J.; Lin, Q.; Luo, F.; Zhou, W.; Zeng, X. Evaluation of the Genotoxic and Oxidative Damage Potential of Silver Nanoparticles in Human NCM460 and HCT116 Cells. Int. J. Mol. Sci. 2020, 21, 1618. https://doi.org/10.3390/ijms21051618

AMA Style

Jia M, Zhang W, He T, Shu M, Deng J, Wang J, Li W, Bai J, Lin Q, Luo F, Zhou W, Zeng X. Evaluation of the Genotoxic and Oxidative Damage Potential of Silver Nanoparticles in Human NCM460 and HCT116 Cells. International Journal of Molecular Sciences. 2020; 21(5):1618. https://doi.org/10.3390/ijms21051618

Chicago/Turabian Style

Jia, Mingxi, Wenjing Zhang, Taojin He, Meng Shu, Jing Deng, Jianhui Wang, Wen Li, Jie Bai, Qinlu Lin, Feijun Luo, Wenhua Zhou, and Xiaoxi Zeng. 2020. "Evaluation of the Genotoxic and Oxidative Damage Potential of Silver Nanoparticles in Human NCM460 and HCT116 Cells" International Journal of Molecular Sciences 21, no. 5: 1618. https://doi.org/10.3390/ijms21051618

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