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Article

Isolation of Four Lytic Phages Infecting Klebsiella pneumoniae K22 Clinical Isolates from Spain

1
Department of Genetics, Institute for Integrative Systems Biology, I2SysBio, Universitat de València, Universitat de València-CSIC, 46980 Paterna, Spain
2
Institute for Integrative Systems Biology, I2SysBio, FISABIO-Salud Pública, Generalitat Valenciana, Universitat de València-CSIC, 46980 Paterna, Spain
3
College West Flanders, Howest University, 8500 Kortrijk, Belgium
4
FISABIO-Salud Pública, Generalitat Valenciana, and CIBER in Epidemiology and Public Health, Institute for Integrative Systems Biology, I2SysBio, Universitat de València-CSIC, 46980 Paterna, Spain
5
Institute for Integrative Systems Biology, I2SysBio, Universitat de València-CSIC, 46980 Paterna, Spain
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(2), 425; https://doi.org/10.3390/ijms21020425
Received: 5 December 2019 / Revised: 28 December 2019 / Accepted: 7 January 2020 / Published: 9 January 2020
(This article belongs to the Special Issue Bacteriophage—Molecular Studies)
The emergence of multi-drug-resistant bacteria represents a major public-health threat. Phages constitute a promising alternative to chemical antibiotics due to their high host specificity, abundance in nature, and evolvability. However, phage host specificity means that highly diverse bacterial species are particularly difficult to target for phage therapy. This is the case of Klebsiella pneumoniae, which presents a hypervariable extracellular matrix capsule exhibiting dozens of variants. Here, we report four novel phages infecting K. pneumoniae capsular type K22 which were isolated from environmental samples in Valencia, Spain. Full genome sequencing showed that these phages belong to the Podoviridae family and encode putative depolymerases that allow digestion of specific K22 K. pneumoniae capsules. Our results confirm the capsular type-specificity of K. pneumoniae phages, as indicated by their narrow infectivity in a panel of K. pneumoniae clinical isolates. Nonetheless, this work represents a step forward in the characterization of phage diversity, which may culminate in the future use of large panels of phages for typing and/or for combating multi-drug-resistant K. pneumoniae. View Full-Text
Keywords: Klebsiella pneumoniae; bacteriophage; phage therapy Klebsiella pneumoniae; bacteriophage; phage therapy
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MDPI and ACS Style

Domingo-Calap, P.; Beamud, B.; Vienne, J.; González-Candelas, F.; Sanjuán, R. Isolation of Four Lytic Phages Infecting Klebsiella pneumoniae K22 Clinical Isolates from Spain. Int. J. Mol. Sci. 2020, 21, 425. https://doi.org/10.3390/ijms21020425

AMA Style

Domingo-Calap P, Beamud B, Vienne J, González-Candelas F, Sanjuán R. Isolation of Four Lytic Phages Infecting Klebsiella pneumoniae K22 Clinical Isolates from Spain. International Journal of Molecular Sciences. 2020; 21(2):425. https://doi.org/10.3390/ijms21020425

Chicago/Turabian Style

Domingo-Calap, Pilar, Beatriz Beamud, Justine Vienne, Fernando González-Candelas, and Rafael Sanjuán. 2020. "Isolation of Four Lytic Phages Infecting Klebsiella pneumoniae K22 Clinical Isolates from Spain" International Journal of Molecular Sciences 21, no. 2: 425. https://doi.org/10.3390/ijms21020425

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