Hydroxytyrosol Inhibits Protein Oligomerization and Amyloid Aggregation in Human Insulin
1
Department of Precision Medicine, Università degli Studi della Campania “Luigi Vanvitelli”, Via L. De Crecchio 7, 80138 Naples, Italy
2
Institute of Biophysics, National Research Council, Via Ugo La Malfa, 153, 90146 Palermo, Italy
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(13), 4636; https://doi.org/10.3390/ijms21134636
Received: 3 June 2020
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Revised: 22 June 2020
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Accepted: 29 June 2020
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Published: 30 June 2020
(This article belongs to the Special Issue Natural Inhibitors of Amyloid Aggregation)
Hydroxytyrosol (HT), one of the main phenolic components of olive oil, has attracted considerable interest for its biological properties, including a remarkable antioxidant and anti-inflammatory power and, recently, for its ability to interfere with the amyloid aggregation underlying several human diseases. We report here a broad biophysical approach and cell biology techniques that allowed us to characterize the molecular mechanisms by which HT affects insulin amyloid aggregation and the related cytotoxicity. Our data show that HT is able to fully inhibit insulin amyloid aggregation and this property seems to be ascribed to the stabilization of the insulin monomeric state. Moreover, HT completely reverses the toxic effect produced by amyloid insulin aggregates in neuroblastoma cell lines by fully inhibiting the production of toxic amyloid species. These findings suggest that the beneficial effects of olive oil polyphenols, including HT, may arise from multifunctional activities and suggest possible a application of this natural compound in the prevention or treatment of amyloid-associated diseases.
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Keywords:
amyloid aggregation; human insulin; hydroxytyrosol; amyloid toxicity
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MDPI and ACS Style
Sirangelo, I.; Borriello, M.; Vilasi, S.; Iannuzzi, C. Hydroxytyrosol Inhibits Protein Oligomerization and Amyloid Aggregation in Human Insulin. Int. J. Mol. Sci. 2020, 21, 4636. https://doi.org/10.3390/ijms21134636
AMA Style
Sirangelo I, Borriello M, Vilasi S, Iannuzzi C. Hydroxytyrosol Inhibits Protein Oligomerization and Amyloid Aggregation in Human Insulin. International Journal of Molecular Sciences. 2020; 21(13):4636. https://doi.org/10.3390/ijms21134636
Chicago/Turabian StyleSirangelo, Ivana, Margherita Borriello, Silvia Vilasi, and Clara Iannuzzi. 2020. "Hydroxytyrosol Inhibits Protein Oligomerization and Amyloid Aggregation in Human Insulin" International Journal of Molecular Sciences 21, no. 13: 4636. https://doi.org/10.3390/ijms21134636
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