Next Article in Journal
Acquisition of the Midbrain Dopaminergic Neuronal Identity
Previous Article in Journal
Contribution of Immune Cells to Glucocorticoid Receptor Expression in Breast Cancer
Previous Article in Special Issue
The Impact of Natural Compounds on S-Shaped Aβ42 Fibril: From Molecular Docking to Biophysical Characterization
Open AccessArticle

Hydroxytyrosol Inhibits Protein Oligomerization and Amyloid Aggregation in Human Insulin

Department of Precision Medicine, Università degli Studi della Campania “Luigi Vanvitelli”, Via L. De Crecchio 7, 80138 Naples, Italy
Institute of Biophysics, National Research Council, Via Ugo La Malfa, 153, 90146 Palermo, Italy
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(13), 4636;
Received: 3 June 2020 / Revised: 22 June 2020 / Accepted: 29 June 2020 / Published: 30 June 2020
(This article belongs to the Special Issue Natural Inhibitors of Amyloid Aggregation)
Hydroxytyrosol (HT), one of the main phenolic components of olive oil, has attracted considerable interest for its biological properties, including a remarkable antioxidant and anti-inflammatory power and, recently, for its ability to interfere with the amyloid aggregation underlying several human diseases. We report here a broad biophysical approach and cell biology techniques that allowed us to characterize the molecular mechanisms by which HT affects insulin amyloid aggregation and the related cytotoxicity. Our data show that HT is able to fully inhibit insulin amyloid aggregation and this property seems to be ascribed to the stabilization of the insulin monomeric state. Moreover, HT completely reverses the toxic effect produced by amyloid insulin aggregates in neuroblastoma cell lines by fully inhibiting the production of toxic amyloid species. These findings suggest that the beneficial effects of olive oil polyphenols, including HT, may arise from multifunctional activities and suggest possible a application of this natural compound in the prevention or treatment of amyloid-associated diseases. View Full-Text
Keywords: amyloid aggregation; human insulin; hydroxytyrosol; amyloid toxicity amyloid aggregation; human insulin; hydroxytyrosol; amyloid toxicity
Show Figures

Graphical abstract

MDPI and ACS Style

Sirangelo, I.; Borriello, M.; Vilasi, S.; Iannuzzi, C. Hydroxytyrosol Inhibits Protein Oligomerization and Amyloid Aggregation in Human Insulin. Int. J. Mol. Sci. 2020, 21, 4636.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Search more from Scilit
Back to TopTop