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Article

3D-Printed Bioactive Calcium Silicate/Poly-ε-Caprolactone Bioscaffolds Modified with Biomimetic Extracellular Matrices for Bone Regeneration

1
School of Medicine, China Medical University, Taichung 40447, Taiwan
2
3D Printing Medical Research Center, China Medical University Hospital, Taichung 40447, Taiwan
3
Department of Orthopedics, China Medical University Hospital, Taichung 40447, Taiwan
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The Ph.D. Program for Medical Engineering and Rehabilitation Science, China Medical University, Taichung 40447, Taiwan
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School of Dentistry, China Medical University, Taichung 40447, Taiwan
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Department of Bioinformatics and Medical Engineering, Asia University, Taichung 40447, Taiwan
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Graduate Institute of Biomedical Sciences, China Medical University, Taichung 40447, Taiwan
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3D Printing Medical Research Institute, Asia University, Taichung 40447, Taiwan
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2019, 20(4), 942; https://doi.org/10.3390/ijms20040942
Received: 26 January 2019 / Revised: 19 February 2019 / Accepted: 19 February 2019 / Published: 21 February 2019
(This article belongs to the Special Issue Biomaterials for Soft and Hard Tissue Regeneration)
Currently, clinically available orthopedic implants are extremely biocompatible but they lack specific biological characteristics that allow for further interaction with surrounding tissues. The extracellular matrix (ECM)-coated scaffolds have received considerable interest for bone regeneration due to their ability in upregulating regenerative cellular behaviors. This study delves into the designing and fabrication of three-dimensional (3D)-printed scaffolds that were made out of calcium silicate (CS), polycaprolactone (PCL), and decellularized ECM (dECM) from MG63 cells, generating a promising bone tissue engineering strategy that revolves around the concept of enhancing osteogenesis by creating an osteoinductive microenvironment with osteogenesis-promoting dECM. We cultured MG63 on scaffolds to obtain a dECM-coated CS/PCL scaffold and further studied the biological performance of the dECM hybrid scaffolds. The results indicated that the dECM-coated CS/PCL scaffolds exhibited excellent biocompatibility and effectively enhanced cellular adhesion, proliferation, and differentiation of human Wharton’s Jelly mesenchymal stem cells by increasing the expression of osteogenic-related genes. They also presented anti-inflammatory characteristics by showing a decrease in the expression of tumor necrosis factor-alpha (TNF-α) and interleukin-1 (IL-1). Histological analysis of in vivo experiments presented excellent bone regenerative capabilities of the dECM-coated scaffold. Overall, our work presented a promising technique for producing bioscaffolds that can augment bone tissue regeneration in numerous aspects. View Full-Text
Keywords: calcium silicate; polycaprolactone; 3D scaffold; decellularized; extracellular matrix; osteogenesis calcium silicate; polycaprolactone; 3D scaffold; decellularized; extracellular matrix; osteogenesis
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MDPI and ACS Style

Wu, Y.-H.A.; Chiu, Y.-C.; Lin, Y.-H.; Ho, C.-C.; Shie, M.-Y.; Chen, Y.-W. 3D-Printed Bioactive Calcium Silicate/Poly-ε-Caprolactone Bioscaffolds Modified with Biomimetic Extracellular Matrices for Bone Regeneration. Int. J. Mol. Sci. 2019, 20, 942. https://doi.org/10.3390/ijms20040942

AMA Style

Wu Y-HA, Chiu Y-C, Lin Y-H, Ho C-C, Shie M-Y, Chen Y-W. 3D-Printed Bioactive Calcium Silicate/Poly-ε-Caprolactone Bioscaffolds Modified with Biomimetic Extracellular Matrices for Bone Regeneration. International Journal of Molecular Sciences. 2019; 20(4):942. https://doi.org/10.3390/ijms20040942

Chicago/Turabian Style

Wu, Yuan-Haw A., Yung-Cheng Chiu, Yen-Hong Lin, Chia-Che Ho, Ming-You Shie, and Yi-Wen Chen. 2019. "3D-Printed Bioactive Calcium Silicate/Poly-ε-Caprolactone Bioscaffolds Modified with Biomimetic Extracellular Matrices for Bone Regeneration" International Journal of Molecular Sciences 20, no. 4: 942. https://doi.org/10.3390/ijms20040942

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