Next Article in Journal
Insights into Body Size Evolution: A Comparative Transcriptome Study on Three Species of Asian Sisoridae Catfish
Next Article in Special Issue
The Proteomic Analysis of Maize Endosperm Protein Enriched by Phos-tagtm Reveals the Phosphorylation of Brittle-2 Subunit of ADP-Glc Pyrophosphorylase in Starch Biosynthesis Process
Previous Article in Journal
3D-Printed Bioactive Calcium Silicate/Poly-ε-Caprolactone Bioscaffolds Modified with Biomimetic Extracellular Matrices for Bone Regeneration
Previous Article in Special Issue
Proteomics Analysis of E. angustifolia Seedlings Inoculated with Arbuscular Mycorrhizal Fungi under Salt Stress
Open AccessArticle

Root Proteomics Reveals the Effects of Wood Vinegar on Wheat Growth and Subsequent Tolerance to Drought Stress

1
College of Agronomy, Northwest A&F University, Yangling 712100, China
2
Northwest Research Center of Rural Renewable Energy, Exploitation and Utilization of Ministry of Agriculture, Northwest A&F University, Yangling 712100, China
3
College of Mechanical and Electronic Engineering, Northwest A&F University, Yangling 712100, China
4
Hubei Key Laboratory of Waterlogging Disaster and Agricultural Use of Wetland, College of Agronomy, Yangtze University, Jingzhou 434025, China
5
Biogas Institute of Ministry of Agriculture and Rural Affairs, Chinese Academy of Agricultural Science, Chengdu 610041, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(4), 943; https://doi.org/10.3390/ijms20040943
Received: 2 February 2019 / Accepted: 19 February 2019 / Published: 21 February 2019
Wood vinegar (WV) or pyroligneous acid (PA) is a reddish-brown liquid created during the dry distillation of biomass, a process called pyrolysis. WV contains important biologically active components, which can enhance plant growth and tolerance to drought stress. However, its mechanism of action remains unknown. Our results after presoaking wheat seeds with various concentrations of WV indicate that a 1:900 WV concentration can significantly enhance growth. To investigate the response of wheat roots to drought stress, we compared quantitative proteomic profiles in the roots of wheat plants grown from seeds either presoaked (treatment) or non-presoaked (control) with WV. Our results indicated that the abscisic acid (ABA) content of wheat roots in the WV treatment was significantly increased. Reactive oxygen species (ROS) and malonaldehyde (MDA) levels roots were significantly lower than in the control treatment under drought stress, while the activity of major antioxidant enzymes was significantly increased. Two-dimensional electrophoresis (2D-PAGE) identified 138 differentially accumulated protein (DAP) spots representing 103 unique protein species responding to drought stress in wheat roots of the control and WV-treated groups. These DAPs are mostly involved in the stress response, carbohydrate metabolism, protein metabolism, and secondary metabolism. Proteome profiles showed the DAPs involved in carbohydrate metabolism, stress response, and secondary metabolism had increased accumulation in roots of the WV-treated groups. These findings suggest that the roots from wheat seeds presoaked with WV can initiate an early defense mechanism to mitigate drought stress. These results provide an explanation of how WV enhances the tolerance of wheat plants to drought stress. View Full-Text
Keywords: wheat; root; wood vinegar; drought stress; ROS; ABA; proteome wheat; root; wood vinegar; drought stress; ROS; ABA; proteome
Show Figures

Figure 1

MDPI and ACS Style

Wang, Y.; Qiu, L.; Song, Q.; Wang, S.; Wang, Y.; Ge, Y. Root Proteomics Reveals the Effects of Wood Vinegar on Wheat Growth and Subsequent Tolerance to Drought Stress. Int. J. Mol. Sci. 2019, 20, 943.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop