Next Article in Journal
ESeroS-GS Protects Neuronal Cells from Oxidative Stress by Stabilizing Lysosomes
Previous Article in Journal
Catalysts with Cerium in a Membrane Reactor for the Removal of Formaldehyde Pollutant from Water Effluents
Open AccessArticle

Bioactive Constituents of Zanthoxylum rhetsa Bark and Its Cytotoxic Potential against B16-F10 Melanoma Cancer and Normal Human Dermal Fibroblast (HDF) Cell Lines

1
Laboratory of Natural Products, Institute of Bioscience, Universiti Putra Malaysia, UPM Serdang 43400, Selangor, Malaysia
2
Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, UPM Serdang 43400, Selangor, Malaysia
*
Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Molecules 2016, 21(6), 652; https://doi.org/10.3390/molecules21060652
Received: 1 April 2016 / Revised: 7 May 2016 / Accepted: 11 May 2016 / Published: 24 May 2016
(This article belongs to the Section Natural Products Chemistry)
Zanthoxylum rhetsa is an aromatic tree, known vernacularly as “Indian Prickly Ash”. It has been predominantly used by Indian tribes for the treatment of many infirmities like diabetes, inflammation, rheumatism, toothache and diarrhea. In this study, we identified major volatile constituents present in different solvent fractions of Z. rhetsa bark using GC-MS analysis and isolated two tetrahydrofuran lignans (yangambin and kobusin), a berberine alkaloid (columbamine) and a triterpenoid (lupeol) from the bioactive chloroform fraction. The solvent fractions and purified compounds were tested for their cytotoxic potential against human dermal fibroblasts (HDF) and mouse melanoma (B16-F10) cells, using the MTT assay. All the solvent fractions and purified compounds were found to be non-cytotoxic to HDF cells. However, the chloroform fraction and kobusin exhibited cytotoxic effect against B16-F10 melanoma cells. The presence of bioactive lignans and alkaloids were suggested to be responsible for the cytotoxic property of Z. rhetsa bark against B16-F10 cells. View Full-Text
Keywords: Zanthoxylum rhetsa; lignans; triterpenes; alkaloids; cytotoxicity; NMR spectroscopy; GC-MS Zanthoxylum rhetsa; lignans; triterpenes; alkaloids; cytotoxicity; NMR spectroscopy; GC-MS
Show Figures

Graphical abstract

MDPI and ACS Style

Santhanam, R.K.; Ahmad, S.; Abas, F.; Safinar Ismail, I.; Rukayadi, Y.; Tayyab Akhtar, M.; Shaari, K. Bioactive Constituents of Zanthoxylum rhetsa Bark and Its Cytotoxic Potential against B16-F10 Melanoma Cancer and Normal Human Dermal Fibroblast (HDF) Cell Lines. Molecules 2016, 21, 652.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop