Metabolite Analysis of Toosendanin by an Ultra-High Performance Liquid Chromatography-Quadrupole-Time of Flight Mass Spectrometry Technique
Abstract
:1. Introduction
2. Results and Discussion
2.1. Mass Spectrometric Characterization of Toosendanin
2.2. Metabolism of Toosendanin in Vitro by Human Liver Microsomes
2.2.1. Quantification of Toosendanin by UHPLC-QQQ/MS
2.2.2. Identification of Metabolites by UHPLC-Q-TOF/MS
3. Experimental
3.1. Chemicals and Reagents
3.2. Incubation of Toosendanin In Vitro with Human Liver Microsomes
3.3. Quantitative Analysis of Toosendanin by UHPLC-QQQ/MS
3.4. Qualitative Analysis by UHPLC-Q-TOF/MS
4. Conclusions
Supplementary Materials
Acknowledgments
Conflicts of Interest
References
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Wu, J.-L.; Leung, E.L.-H.; Zhou, H.; Liu, L.; Li, N. Metabolite Analysis of Toosendanin by an Ultra-High Performance Liquid Chromatography-Quadrupole-Time of Flight Mass Spectrometry Technique. Molecules 2013, 18, 12144-12153. https://doi.org/10.3390/molecules181012144
Wu J-L, Leung EL-H, Zhou H, Liu L, Li N. Metabolite Analysis of Toosendanin by an Ultra-High Performance Liquid Chromatography-Quadrupole-Time of Flight Mass Spectrometry Technique. Molecules. 2013; 18(10):12144-12153. https://doi.org/10.3390/molecules181012144
Chicago/Turabian StyleWu, Jian-Lin, Elaine Lai-Han Leung, Hua Zhou, Liang Liu, and Na Li. 2013. "Metabolite Analysis of Toosendanin by an Ultra-High Performance Liquid Chromatography-Quadrupole-Time of Flight Mass Spectrometry Technique" Molecules 18, no. 10: 12144-12153. https://doi.org/10.3390/molecules181012144