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11 Results Found

  • Article
  • Open Access
10 Citations
3,782 Views
14 Pages

27 January 2023

The enzymatic transglycosylation of steviol glycosides can improve the edulcorant quality of steviol glycosides. Cyclodextrin glucanotransferase (CGTase) is one of the most popular glucanotransferases applied in this reaction. Herein, the CGTase-prod...

  • Article
  • Open Access
25 Citations
12,369 Views
10 Pages

9 February 2010

The production of hesperetin glycosides was investigated using glycosylation with Xanthomonas campestris and cyclodextrin glucanotransferase (CGTase). X. campestris glucosylated hesperetin to its 3'-, 5-, and 7-O-glucosides, and CGTase converted hesp...

  • Article
  • Open Access
21 Citations
4,973 Views
12 Pages

6 March 2019

Cyclodextrin glucanotransferases (CGTases) convert starch to cyclodextrins (CD) of various sizes. To engineer a CGTase for the synthesis of large-ring CD composed of 9 to 12 glucose units, a loop structure of the protein involved in substrate binding...

  • Article
  • Open Access
15 Citations
10,509 Views
9 Pages

20 August 2009

The glycosylation of α- and δ-tocopherols using Klebsiella pneumoniae and cyclodextrin glucanotransferase (CGTase) was investigated. K. pneumoniae converted α- and δ-tocopherols into the corresponding β-glucosides in 10 and 8% yield, respectively. CG...

  • Article
  • Open Access
24 Citations
8,990 Views
9 Pages

29 July 2010

The production of β-maltooligosaccharides of glycitein and daidzein using Lactobacillus delbrueckii and cyclodextrin glucanotransferase (CGTase) as biocatalysts was investigated. The cells of L. delbrueckii glucosylated glycitein and daidzein to give...

  • Article
  • Open Access
11 Citations
4,508 Views
13 Pages

Production and Surfactant Properties of Tert-Butyl α-d-Glucopyranosides Catalyzed by Cyclodextrin Glucanotransferase

  • Humberto Garcia-Arellano,
  • Jose L. Gonzalez-Alfonso,
  • Claudia Ubilla,
  • Francesc Comelles,
  • Miguel Alcalde,
  • Manuel Bernabé,
  • José-Luis Parra,
  • Antonio O. Ballesteros and
  • Francisco J. Plou

29 June 2019

While testing the ability of cyclodextrin glucanotransferases (CGTases) to glucosylate a series of flavonoids in the presence of organic cosolvents, we found out that this enzyme was able to glycosylate a tertiary alcohol (tert-butyl alcohol). In par...

  • Article
  • Open Access
28 Citations
7,989 Views
14 Pages

Enzymatic Synthesis of a Novel Pterostilbene α-Glucoside by the Combination of Cyclodextrin Glucanotransferase and Amyloglucosidase

  • José L. González-Alfonso,
  • David Rodrigo-Frutos,
  • Efres Belmonte-Reche,
  • Pablo Peñalver,
  • Ana Poveda,
  • Jesús Jiménez-Barbero,
  • Antonio O. Ballesteros,
  • Yoshihiko Hirose,
  • Julio Polaina and
  • Francisco J. Plou
  • + 1 author

The synthesis of a novel α-glucosylated derivative of pterostilbene was performed by a transglycosylation reaction using starch as glucosyl donor, catalyzed by cyclodextrin glucanotransferase (CGTase) from Thermoanaerobacter sp. The reaction wa...

  • Article
  • Open Access
20 Citations
9,052 Views
11 Pages

9 June 2011

Rutin was subjected to intermolecular transglycosylation assisted with microwave irradiation using cyclodextrin glucanotransferase (CGTase) produced from Bacillus sp. SK13.002. Compared with the conventional enzymatic method for rutin transglycosylat...

  • Article
  • Open Access
25 Citations
5,590 Views
11 Pages

Optimization of Regioselective α-Glucosylation of Hesperetin Catalyzed by Cyclodextrin Glucanotransferase

  • José L. González-Alfonso,
  • Noa Míguez,
  • J. Daniel Padilla,
  • Laura Leemans,
  • Ana Poveda,
  • Jesús Jiménez-Barbero,
  • Antonio O. Ballesteros,
  • Georgina Sandoval and
  • Francisco J. Plou

5 November 2018

The regioselective α-glucosylation of hesperetin was achieved by a transglycosylation reaction catalyzed by cyclodextrin glucanotransferase (CGTase) from Thermoanaerobacter sp. using soluble starch as glucosyl donor. By combining mass spectrometry (E...

  • Article
  • Open Access
33 Citations
6,422 Views
10 Pages

Development of Freeze-Thaw Stable Starch through Enzymatic Modification

  • Seung-Hye Woo,
  • Ji-Soo Kim,
  • Hyun-Mo Jeong,
  • Yu-Jeong Shin,
  • Jung-Sun Hong,
  • Hee-Don Choi and
  • Jae-Hoon Shim

25 September 2021

The use of unmodified starch in frozen foods can cause extremely undesirable textural changes after the freeze-thaw process. In this study, using cyclodextrin glucanotransferase (CGTase) and branching enzymes, an amylopectin cluster with high freeze-...

  • Article
  • Open Access
1 Citations
2,578 Views
19 Pages

Binding Specificity of a Novel Cyclo/Maltodextrin-Binding Protein and Its Role in the Cyclodextrin ABC Importer System from Thermoanaerobacterales

  • Jorge Aranda-Caraballo,
  • Roberto A. Saenz,
  • Alonso A. López-Zavala,
  • Beatriz Velazquez-Cruz,
  • Laura Espinosa-Barrera,
  • Yair Cárdenas-Conejo,
  • Andrés Zárate-Romero,
  • Oscar Linares-Vergara,
  • Juan A. Osuna-Castro and
  • Hugo Serrano-Posada
  • + 1 author

16 August 2023

Extracellular synthesis of functional cyclodextrins (CDs) as intermediates of starch assimilation is a convenient microbial adaptation to sequester substrates, increase the half-life of the carbon source, carry bioactive compounds, and alleviate chem...