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Italian Society of the Veterinary Sciences SISVet 2018
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Italian Society of the Veterinary Sciences SISVet 2018

A special issue of Veterinary Sciences (ISSN 2306-7381).

Deadline for manuscript submissions: closed (20 January 2019) | Viewed by 57538

Special Issue Editors

Prof. Luigi Bertolotti

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Guest Editor
Diagnostic-Public Health-Pathological Anatomy Rellated to Infectious Disease, Department of Veterinary Science, University of Torino Largo, Braccini 2, 10095 Grugliasco, Italy
Interests: biology and molecular virology; inferential statistics; biostatistics and bioinformatics; molecular epidemiology and evolution; computational epidemiology; mathematical models of diffusion of pathogens
Prof. Dr. Francesco Staffieri

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Guest Editor
Section of Veterinary Clinics and Animal Production, Department of Precision and Regenerative Medicine and Ionian Area, University of Bari “Aldo Moro”, SP 62 per Casamassima km 3, 70010 Valenzano, Bari, Italy
Interests: pain in small and large animals; analgesia; osteoarthritis; experimental animal models of ALI/ARDS; pulmonary atelectasis and its treatment during anesthesia; non invasive ventilation in animal patients
Special Issues, Collections and Topics in MDPI journals
Prof. Dr. Orlando Paciello

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Guest Editor
Department of Veterinary Medicine and Animal Production, University of Naples Federico II, Via Federico Delpino 1, 80137 Naples, Italy
Interests: veterinary pathology; veterinary forensic medicine; neuromuscular disorders; muscle and brain aging; environmental pathology
Special Issues, Collections and Topics in MDPI journals
Prof. Dr. Emanuela Valle

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Guest Editor
Department of Veterinary Sciences, University of Turin, Largo Paolo Braccini 2, 10095 Grugliasco, Turin, Italy
Interests: equine nutrition; welfare; gut health; applied clinical nutrition; feeding behavior and ethological need; feeding practice
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

We are happy to invite you to the SISVet 2018 Congress that is the biggest conference in Italy dedicated to veterinary science, which has been held for over 72 years. The conference will be held 20–22 June in Torino. The conference will include the participation of the following societoes: SOFIVET Italian Society of Veterinary Physiology, SICV Italian Society of Veterinary Surgery, SIRA Italian Society of Animal Reproduction, AIPVET Italian Association of Veterinary Pathology, ARNA, Association of Researchers Nutrition and Food, ANIV Italian Association of Veterinary Infectivologists, RNIV, and the National Network of Veterinary Immunology.

We are particularly interested in articles related to the society involved and to the central theme of the convention, which will be antimicrobial resistance in veterinary implications, from pharmacological, infectious, clinical, zootechnical, pathological, and public health aspects. Naturally, all topics related to veterinary sciences are welcome.  The novelty of this year is represented by the ARNA that will propose for its scientific session the theme: "Milk in the Diet of the Future".

On behalf of the organizing committee, we welcome your contribution.

Prof. Luigi Bertolotti
Prof. Francesco Staffieri
Prof. Orlando Paciello
Dr. Emanuela Valle
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Veterinary Sciences is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2100 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • one health
  • veterinary science
  • antimicrobial resistance
  • pharmacological
  • infectious
  • clinical
  • zootechnical
  • pathological
  • public health

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Published Papers (12 papers)

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Research

12 pages, 7787 KiB  
Article
Evaluation of in Vivo Response of Three Biphasic Scaffolds for Osteochondral Tissue Regeneration in a Sheep Model
by Alberto M. Crovace, Alessia Di Giancamillo, Francesca Gervaso, Laura Mangiavini, Davide Zani, Francesca Scalera, Barbara Palazzo, Daniela Izzo, Marco Agnoletto, Marco Domenicucci, Corrado Sosio, Alessandro Sannino, Mauro Di Giancamillo and Giuseppe M. Peretti
Vet. Sci. 2019, 6(4), 90; https://doi.org/10.3390/vetsci6040090 - 9 Nov 2019
Cited by 7 | Viewed by 4279
Abstract
Osteochondral defects are a common problem in both human medicine and veterinary practice although with important limits concerning the cartilaginous tissue regeneration. Interest in the subchondral bone has grown, as it is now considered a key element in the osteochondral defect healing. The [...] Read more.
Osteochondral defects are a common problem in both human medicine and veterinary practice although with important limits concerning the cartilaginous tissue regeneration. Interest in the subchondral bone has grown, as it is now considered a key element in the osteochondral defect healing. The aim of this work was to generate and to evaluate the architecture of three cell-free scaffolds made of collagen, magnesium/hydroxyapatite and collagen hydroxyapatite/wollastonite to be implanted in a sheep animal model. Scaffolds were designed in a bilayer configuration and a novel “Honey” configuration, where columns of hydroxyapatite were inserted within the collagen matrix. The use of different types of scaffolds allowed us to identify the best scaffold in terms of integration and tissue regeneration. The animals included were divided into four groups: three were treated using different types of scaffold while one was left untreated and represented the control group. Evaluations were made at 3 months through CT analysis. The novel “Honey” configuration of the scaffold with hydroxyapatite seems to allow for a better reparative process, although we are still far from obtaining a complete restoration of the defect at this time point of follow-up. Full article
(This article belongs to the Special Issue Italian Society of the Veterinary Sciences SISVet 2018)
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14 pages, 507 KiB  
Article
Volatilome in Milk for Grana Padano and Parmigiano Reggiano Cheeses: A First Survey
by Massimo Faustini, Giovanni Quintavalle Pastorino, Carla Colombani, Luca Maria Chiesa, Sara Panseri, Daniele Vigo and Giulio Curone
Vet. Sci. 2019, 6(2), 41; https://doi.org/10.3390/vetsci6020041 - 9 May 2019
Cited by 7 | Viewed by 4274
Abstract
Milk characteristics in terms of volatile compounds can influence the subsequent product characteristics and can give indications about metabolism. These features can strongly depend on feeding and management. In this perspective, the screening of milk samples intended for Parmigiano-Reggiano and Grana Padano located [...] Read more.
Milk characteristics in terms of volatile compounds can influence the subsequent product characteristics and can give indications about metabolism. These features can strongly depend on feeding and management. In this perspective, the screening of milk samples intended for Parmigiano-Reggiano and Grana Padano located in Northern Italy was performed, focusing on a panel of volatile molecules. The work was carried out on a total number of 25 bovine milk samples for the production of Parmigiano-Reggiano and Grana Padano. Milk samples were collected from May to September and analyzed for volatile molecules using headspace gas chromatography mass spectrometry. A range of several volatile molecule classes was considered (aldehydes, ketones, alcohols, carboxylic acids, esters, aromatic hydrocarbons, solforates). Results showed a significant influence of the month and destination of milk due to the time period and subsequent use in cheesemaking (Parmigiano-Reggiano and Grana Padano). Significant differences between months were observed for all volatiles. These preliminary results indicate differences between the two types of milk due to the period and destination. The study of volatile molecules in milk will give important information about the physiology of milk and the evolution of dairy products. These features must be extended and confirmed by the sensory analysis of milk and derived products, leading to a more complete characterization of milk biology and derived products. Full article
(This article belongs to the Special Issue Italian Society of the Veterinary Sciences SISVet 2018)
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8 pages, 1804 KiB  
Article
Assessment of the Exposure of People to Questing Ticks Carrying Agents of Zoonoses in Aosta Valley, Italy
by Ilary Millet, Marco Ragionieri, Laura Tomassone, Claudio Trentin and Alessandro Mannelli
Vet. Sci. 2019, 6(1), 28; https://doi.org/10.3390/vetsci6010028 - 17 Mar 2019
Cited by 10 | Viewed by 3994
Abstract
We estimated the probability of exposure of people to questing ticks, infected with bacterial agents of the tick—borne zoonoses—in Aosta Valley, western Alps, Italy. We collected ticks by dragging, and from collectors’ clothes in three hiking trails, which were divided into an internal [...] Read more.
We estimated the probability of exposure of people to questing ticks, infected with bacterial agents of the tick—borne zoonoses—in Aosta Valley, western Alps, Italy. We collected ticks by dragging, and from collectors’ clothes in three hiking trails, which were divided into an internal path, with short vegetation, and an external part with taller grass. Dragging yielded 285 Ixodes ricinus nymphs and 31 adults, and two Dermacentor marginatus adults. Eleven I. ricinus nymphs and 9 adults were collected from collectors’ clothes. Borrelia burgdorferi s.l. was identified by PCR in 12 out of 30 I. ricinus nymphs (prevalence = 40.0%, 95% confidence interval = 22.5, 57.5). The prevalence of infection by Rickettsia spp. was 13.3% (95% CI = 1.2, 25.5). The probability of encountering at least one questing I. ricinus infected by each bacterial agent (probability of exposure, E) in 100 m2 was obtained by combining the number of collected nymphs, the prevalence of infection by each bacterial agent, the frequency of passage by visitors, and the probability of tick attachment to people. The mean number of nymphs collected by dragging was greatest in the internal part of hiking trails (mean = 7.9). Conversely, E was greater in the external part (up to 0.14 for B. burgdorferi s.l., and 0.07 for Rickettsia spp.), due to a greater probability of tick attachment to people in relatively tall vegetation. Full article
(This article belongs to the Special Issue Italian Society of the Veterinary Sciences SISVet 2018)
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7 pages, 830 KiB  
Article
Urinalysis and Urinary GGT-to-Urinary Creatinine Ratio in Dogs with Acute Pancreatitis
by Eleonora Gori, Alessio Pierini, Ilaria Lippi, Noemi Boffa, Francesca Perondi and Veronica Marchetti
Vet. Sci. 2019, 6(1), 27; https://doi.org/10.3390/vetsci6010027 - 13 Mar 2019
Cited by 11 | Viewed by 5895
Abstract
In acute pancreatitis (AP), kidney injury (KI) can occur. Urinalysis and some urinary biomarkers have been proposed as prognostic tools in human AP. The aim of the study was to evaluate urinalysis and urinary GGT-to-urinary creatinine (uGGT/uCr) in canine AP and their association [...] Read more.
In acute pancreatitis (AP), kidney injury (KI) can occur. Urinalysis and some urinary biomarkers have been proposed as prognostic tools in human AP. The aim of the study was to evaluate urinalysis and urinary GGT-to-urinary creatinine (uGGT/uCr) in canine AP and their association with possible outcomes. AP diagnosis was based on clinical and laboratory parameters, abnormal SNAP® cPL™ test and compatible imaging. Urinary KI (uKI) was defined if dogs had urinary casts and/or proteinuria. Dogs (n = 70) were divided in survivors and non-survivors according to the 15-day outcome. Data were analyzed using statistical software. Seventy dogs were retrospectively included, of which 24 dogs (34%) died. uKI was detected in 36 dogs (37%) which was associated with mortality (p = 0.01, Odds ratio (OR) 3.9, 95% CI 1.3–11.56). Non-survivors showed higher dipstick bilirubin levels than survivors (p = 0.0022). By excluding active sediments, urine protein-to-creatinine ratio (UPC) ≥2 was associated with mortality (p = 0.001, OR 47.5, 95% CI 4–571.9). The uGGT/uCr was available in 40 dogs, although no association of this factor with any outcome was found. The UPC ≥2 can be a negative prognostic factor in canine AP and further studies on uGGT/uCr are warranted. Full article
(This article belongs to the Special Issue Italian Society of the Veterinary Sciences SISVet 2018)
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11 pages, 1634 KiB  
Article
Intracoronary Gene Delivery of the Cytoprotective Factor Vascular Endothelial Growth Factor-B167 in Canine Patients with Dilated Cardiomyopathy: A Short-Term Feasibility Study
by Paola Paradies, Lucia Carlucci, Felix Woitek, Francesco Staffieri, Luca Lacitignola, Luigi Ceci, Daniela Romano, Mariateresa Sasanelli, Lorena Zentilin, Mauro Giacca, Stefano Salvadori, Antonio Crovace and Fabio A. Recchia
Vet. Sci. 2019, 6(1), 23; https://doi.org/10.3390/vetsci6010023 - 6 Mar 2019
Cited by 6 | Viewed by 5301
Abstract
Dilated cardiomyopathy (DCM) is a myocardial disease of dogs and humans characterized by progressive ventricular dilation and depressed contractility and it is a frequent cause of heart failure. Conventional pharmacological therapy cannot reverse the progression of the disease and, in humans, cardiac transplantation [...] Read more.
Dilated cardiomyopathy (DCM) is a myocardial disease of dogs and humans characterized by progressive ventricular dilation and depressed contractility and it is a frequent cause of heart failure. Conventional pharmacological therapy cannot reverse the progression of the disease and, in humans, cardiac transplantation remains the only option during the final stages of heart failure. Cytoprotective gene therapy with vascular endothelial growth factor-B167 (VEGF-B167) has proved an effective alternative therapy, halting the progression of the disease in experimental studies on dogs. The aim of this work was to test the tolerability and feasibility of intracoronary administration, under fluoroscopic guidance, of VEGF-B167 carried by adeno-associated viral vectors in canine DCM patients. Ten patients underwent the gene delivery procedure. The intraoperative phase was well tolerated by all dogs. Clinical and echocardiographic assessments at 7- and 30-days post-procedure showed stable conditions compared to the pre-procedure phase. The results of this work indicate that intracoronary VEGF-B167 gene delivery is feasible and tolerated in dogs with DCM. Further monitoring/investigations are ongoing to evaluate the effects of this therapy on disease progression. Full article
(This article belongs to the Special Issue Italian Society of the Veterinary Sciences SISVet 2018)
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9 pages, 1185 KiB  
Article
Attitudes toward Animals and Their Welfare among Italian Veterinary Students
by Federica Pirrone, Chiara Mariti, Angelo Gazzano, Mariangela Albertini, Claudio Sighieri and Silvana Diverio
Vet. Sci. 2019, 6(1), 19; https://doi.org/10.3390/vetsci6010019 - 20 Feb 2019
Cited by 16 | Viewed by 5062
Abstract
As members of the public and the veterinary profession are increasingly concerned about animal welfare, there has been an increased scholarly interest in the attitudes of veterinarians and students toward animals, as these may impact human behavior, which ultimately impacts animal welfare. Here [...] Read more.
As members of the public and the veterinary profession are increasingly concerned about animal welfare, there has been an increased scholarly interest in the attitudes of veterinarians and students toward animals, as these may impact human behavior, which ultimately impacts animal welfare. Here we investigated Italian veterinary students’ demographic data and perceptions about nonhuman animal welfare issues that might be predictive of their attitudes. A survey eliciting information about demographics, knowledge, experience, and perceptions regarding different categories of animals, and including the Animal Attitude Scale (AAS), was administered to undergraduate veterinary medicine students in three Italian universities. Data were analyzed using nonparametric tests, and a value of p < 0.05 was considered statistically significant. In total, 876 students completed the questionnaire, with females (75.1%) making up a majority of students in all years of the course. Although veterinary students showed pro-animal welfare attitudes (mean score = 64.20 ± 0.24 out of 100), the findings suggested that year of study, gender, and geographical location had a significant impact (p < 0.05). In this study, we found a set of factors that, either individually or combined, help predict a student’s attitude toward animal welfare issues, which will be useful in improving the curriculum strategy in veterinary education in Italy. Full article
(This article belongs to the Special Issue Italian Society of the Veterinary Sciences SISVet 2018)
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13 pages, 4824 KiB  
Article
41 Cases of Treatment of Cranial Cruciate Ligament Rupture with Porous TTA: Three Years of Follow Up
by Rodrigo Trisciuzzi, Laura Fracassi, Hernando Afonso Martin, Donato Monopoli Forleo, Daniel Amat, Leonor Santos-Ruiz, Elena De Palma and Alberto Maria Crovace
Vet. Sci. 2019, 6(1), 18; https://doi.org/10.3390/vetsci6010018 - 20 Feb 2019
Cited by 14 | Viewed by 6786
Abstract
Tibial Tuberosity Advancement (TTA) is a surgical technique based on a linear osteotomy that determines a cranial advancement of the tibial tuberosity in patients suffering from cranial cruciate ligament rupture (CCL). The aim is to neutralize the cranial tibial thrust (CTT) and to [...] Read more.
Tibial Tuberosity Advancement (TTA) is a surgical technique based on a linear osteotomy that determines a cranial advancement of the tibial tuberosity in patients suffering from cranial cruciate ligament rupture (CCL). The aim is to neutralize the cranial tibial thrust (CTT) and to reach a 90° angle between the patellar tendon and the tibial plateau with a physiological knee extension of 135°. In our study, a Ti6AI4V ELI (Titanium Aluminium Vanadium) titanium scaffold for the Porous TTA, with excellent properties of osteointegration and osteoconduction when subjected to cyclic loading has been adopted. Based on the previous scientific work on an ovine model, the use of this type of porous scaffolds has subverted the previous models. Scaffold production technology is based on direct mechanical manufacturing called Electron Beam Melting (EBM). For this study, 41 dogs, different breeds, medium-large size, weighing between 10 and 80 kg, aged between 1 and 13 years, were enrolled. The inclusion criteria were based on clinical evaluations (different gaits), drawer test and tibial compression, LOAD score (Liverpool Osteoarthritis in Dogs questionnaire), radiographic diagnosis in sedation with a 135° positioning of the joint and baropodometric investigations (Stance Analyzer). The results show that Porous TTA is an excellent method for functional recovery of the knee joint following the partial and total rupture of the CCL. Full article
(This article belongs to the Special Issue Italian Society of the Veterinary Sciences SISVet 2018)
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8 pages, 432 KiB  
Article
Pulse Pressure Variation Can Predict the Hemodynamic Response to Pneumoperitoneum in Dogs: A Retrospective Study
by Caterina Di Bella, Luca Lacitignola, Laura Fracassi, Despoina Skouropoulou, Antonio Crovace and Francesco Staffieri
Vet. Sci. 2019, 6(1), 17; https://doi.org/10.3390/vetsci6010017 - 20 Feb 2019
Cited by 5 | Viewed by 4623
Abstract
Pneumoperitoneum may induce important hemodynamic alterations in healthy subjects. Pulse pressure variation (PPV) is a hemodynamic parameter able to discriminate preload dependent subjects. Anesthesia records of dogs undergoing laparoscopy were retrospectively evaluated. The anesthetic protocol included acepromazine, methadone, propofol and isoflurane administered with [...] Read more.
Pneumoperitoneum may induce important hemodynamic alterations in healthy subjects. Pulse pressure variation (PPV) is a hemodynamic parameter able to discriminate preload dependent subjects. Anesthesia records of dogs undergoing laparoscopy were retrospectively evaluated. The anesthetic protocol included acepromazine, methadone, propofol and isoflurane administered with oxygen under mechanical ventilation. The hemodynamic parameters were considered five minutes before (BASE) and ten minutes after (P10) the pneumoperitoneum. Based on the cardiac index (CI) variation, at P10, dogs were classified as sensitive (S group, CI ≤ 15%) and non-sensitive (NO-S group). Data were analyzed with the ANOVA test and the ROC curve (p < 0.05). Fifty-five percent of dogs (S) had a reduction of CI ≥ 15% at P10 (2.97 ± 1.4 L/min/m2) compared to BASE (4.32 ± 1.62 L/min/m2) and at P10 in the NO-S group (4.51 ± 1.41 L/min/m2). PPV at BASE was significantly higher in the S group (22.4% ± 6.1%) compared to the NO-S group (10.9% ± 3.3%). The ROC curve showed a threshold of PPV > 16% to distinguish the S and NO-S groups. PPV may be a valid predictor of the hemodynamic response to pneumoperitoneum in dogs. A PPV > 16% can identify patients that may require fluid administration before the creation of pneumoperitoneum. Full article
(This article belongs to the Special Issue Italian Society of the Veterinary Sciences SISVet 2018)
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5 pages, 220 KiB  
Communication
Molecular Detection of Bovine Papillomavirus DNA in the Placenta and Blood of Healthy Mares and Respective Foals
by Federica Savini, Laura Gallina, Francesca Mazza, Jole Mariella, Carolina Castagnetti and Alessandra Scagliarini
Vet. Sci. 2019, 6(1), 14; https://doi.org/10.3390/vetsci6010014 - 6 Feb 2019
Cited by 10 | Viewed by 4206
Abstract
Despite the characteristic species specificity of Papillomaviruses (PVs), the bovine papillomavirus (BPV) types 1, 2, and—more rarely—13, can cross-infect equids, where they are involved in the pathogenesis of sarcoid neoplasms. Sarcoids are locally invasive fibroblastic skin tumors that represent the most common skin [...] Read more.
Despite the characteristic species specificity of Papillomaviruses (PVs), the bovine papillomavirus (BPV) types 1, 2, and—more rarely—13, can cross-infect equids, where they are involved in the pathogenesis of sarcoid neoplasms. Sarcoids are locally invasive fibroblastic skin tumors that represent the most common skin neoplasms in horses worldwide. The transmission mechanism of BPV is still controversial in horses. Thus far, direct and indirect routes have been implicated, while vertical transmission has been suggested after the detection of viral DNA in the semen of healthy stallions. Testing of the blood and placenta of non-sarcoid baring mares and their respective foals revealed that the equine placenta can harbor BPV DNA, leading us to speculate a possible prenatal vertical DNA transmission in equids. Full article
(This article belongs to the Special Issue Italian Society of the Veterinary Sciences SISVet 2018)
16 pages, 290 KiB  
Article
Assessment of the Exposure of Turkey Farmers to Antimicrobial Resistance Associated with Working Practices
by Giorgio Franceschini, Marta Bottino, Ilary Millet, Elisa Martello, Francesca Zaltron, Anna Rosa Favretto, Nicoletta Vonesch, Paola Tomao and Alessandro Mannelli
Vet. Sci. 2019, 6(1), 13; https://doi.org/10.3390/vetsci6010013 - 1 Feb 2019
Cited by 6 | Viewed by 4027
Abstract
The objective of the present study was the identification of farming practices in the production of turkeys for human consumption, and their ranking in terms of the occupational probability of exposure to antimicrobial resistant (AMR) bacteria, for farm workers. We gathered evidence and [...] Read more.
The objective of the present study was the identification of farming practices in the production of turkeys for human consumption, and their ranking in terms of the occupational probability of exposure to antimicrobial resistant (AMR) bacteria, for farm workers. We gathered evidence and data from scientific literature, on risk factors for AMR in farmers, and on the prevalence of those hazards across farming phases. We administered semi-structured interviews to public and private veterinarians in Northern Italy, to obtain detailed information on turkey farming phases, and on working practices. Data were then integrated into a semi-quantitative Failure Modes and Effect Analysis (FMEA). Those working practices, which are characterized by direct contact with numerous animals, and which are carried out frequently, with rare use of personal protection devices resulted as associated with the greatest probability of exposure to AMR. For methicillin resistant Staphylococcus aureus (MRSA), these included vaccination and administration of any individual therapy, and removal and milling of litter, given the exposure of farmers to high dust level. Indeed, levels of occupational exposure to MRSA are enhanced by its transmission routes, which include direct contact with animal, as well as airborne transmission. Level of exposure to extended spectrum beta lactamase (ESBL) is more strictly associated with direct contact and the oral-fecal route. Consequently, exposure to ESBL resulted and associated with the routinely tipping over of poults turned on their back, and with the individual administration of therapies. Full article
(This article belongs to the Special Issue Italian Society of the Veterinary Sciences SISVet 2018)
11 pages, 787 KiB  
Article
Investigation of PAG2 mRNA Expression in Water Buffalo Peripheral Blood Mononuclear Cells and Polymorphonuclear Leukocytes from Maternal Blood at the Peri-Implantation Period
by Olimpia Barbato, Gabriella Guelfi, Laura Menchetti, Gabriele Brecchia, Noelita Melo de Sousa, Claudio Canali, Francesco Grandoni, Maria Carmela Scatà, Giovanna De Matteis, Anna Beatrice Casano, Jean François Beckers and Vittoria Lucia Barile
Vet. Sci. 2019, 6(1), 8; https://doi.org/10.3390/vetsci6010008 - 14 Jan 2019
Cited by 5 | Viewed by 3867
Abstract
The main objective of this study was to assess PAG2 mRNA expression in maternal blood cells at the peri-implantation period in water buffalo; moreover, we wanted to evaluate the earliest time in which PAG-2 could be detected in maternal blood. Thirty-two lactating buffaloes [...] Read more.
The main objective of this study was to assess PAG2 mRNA expression in maternal blood cells at the peri-implantation period in water buffalo; moreover, we wanted to evaluate the earliest time in which PAG-2 could be detected in maternal blood. Thirty-two lactating buffaloes artificially inseminated (AI) were utilized. Blood was collected at Days 0, 14, 18, 28, 40 after AI (AI = day 0). Pregnancy was diagnosed by ultrasound at Days 28 and 40 post AI. Out of 32 buffaloes, 14 were pregnant (P group) and 18 were not pregnant (NP group). The plasma PAG-2 threshold of 1.0 ng/mL in the P group was reached at day 40 post AI. PAG2 mRNA expression differed between the P and NP groups, and was either evaluated in Peripheral Blood Mononuclear Cells (PBMC) or Polymorphonuclear Leukocytes (PMN), starting from day 14. However, both the estimated marginal means and multiple comparisons showed that PAG2 mRNA expression was higher in PMN than PBMC. In the present study, PAG-2 appeared in the blood (40 Days post AI), and an early expression of PAG2 mRNA at Day 14 post AI was also observed. Although further research is undoubtedly required, PAG2 mRNA in peripheral blood leukocytes could be using to better understand the role that PAGs play during pregnancy in buffalo. Full article
(This article belongs to the Special Issue Italian Society of the Veterinary Sciences SISVet 2018)
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10 pages, 1272 KiB  
Article
Simultaneous Quantitative Detection of Six Families of Antibiotics in Honey Using A Biochip Multi-Array Technology
by Roberta Barrasso, Elisabetta Bonerba, Alessandra Emilia Savarino, Edmondo Ceci, Giancarlo Bozzo and Giuseppina Tantillo
Vet. Sci. 2019, 6(1), 1; https://doi.org/10.3390/vetsci6010001 - 28 Dec 2018
Cited by 22 | Viewed by 3920
Abstract
Chemical residues of veterinary drugs such as streptomycin, chloramphenicol, macrolides, sulphonamides, tetracyclines, quinolones and aminoglycosides and other contaminants such as pesticides and heavy metals have been found in honey, leading to concerns for human health. Indeed, there is a growing interest in their [...] Read more.
Chemical residues of veterinary drugs such as streptomycin, chloramphenicol, macrolides, sulphonamides, tetracyclines, quinolones and aminoglycosides and other contaminants such as pesticides and heavy metals have been found in honey, leading to concerns for human health. Indeed, there is a growing interest in their presence and persistence in the environment because low levels of antibiotics may favour the proliferation of antibiotic-resistant bacteria. Moreover, antibiotics present in honey may produce residues in foodstuffs, causing adverse effects on humans such as allergic reactions, toxic effects and damage to the central nervous systems. For food and health/safety reasons, antibiotic drugs are not authorized for the treatment of honey bees in the EU, even though these antimicrobial drugs have been approved in many third-party countries. For this reason, contaminated honey products can still be found in European markets. Therefore, there is a need to develop a precise, accurate and sensitive analytical method that may be used to simply and rapidly detect these compounds in honey. The aim of our study was to detect the presence of antibiotics in Apulian honey using the Anti-Microbial array II (AM II) as an innovative screening method to test the health quality of honey and honey products. Full article
(This article belongs to the Special Issue Italian Society of the Veterinary Sciences SISVet 2018)
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