Toxics

30 pages, 3028 KB

Open AccessArticle

Estimation of Prenatal Alcohol Exposure: Comparison of Retrospective Survey and Measurement of Fatty Acid Ethyl Esters, Ethyl Sulfate, and Ethyl Glucuronide Concentrations in Neonatal Meconium

by Marek Wiergowski, Iwona Jańczewska, Jolanta Wierzba, Monika Cichoń-Kotek, Mateusz Kacper Woźniak, Agata Kot-Wasik, Marek Biziuk, Jacek Sein Anand, Daria Barbara Schetz, Małgorzata Glińska and Katarzyna Hinca

Toxics 2026, 14(2), 155; https://doi.org/10.3390/toxics14020155 - 4 Feb 2026

Abstract

Determining the concentration of fatty acid ethyl esters (FAEEs), ethyl sulfate (EtS), and ethyl glucuronide (EtG) is crucial for establishing the true scale of prenatal alcohol exposure (PAE) and enabling early diagnosis of fetal alcohol spectrum disorders. This study primarily aimed to compare [...] Read more.

Determining the concentration of fatty acid ethyl esters (FAEEs), ethyl sulfate (EtS), and ethyl glucuronide (EtG) is crucial for establishing the true scale of prenatal alcohol exposure (PAE) and enabling early diagnosis of fetal alcohol spectrum disorders. This study primarily aimed to compare two detection methods: retrospective maternal alcohol consumption surveys and chromatographic analysis of newborn meconium. Among 478 mothers, parallel survey data and meconium samples were collected. Nine FAEEs were measured by gas chromatography–mass spectrometry, and EtG and EtS by liquid chromatography–tandem mass spectrometry. The study also aimed to establish marker cut-offs and evaluate their clinical utility. While only 4% (approximately) of mothers reported alcohol consumption during pregnancy, the biomarker analysis suggested a significant underestimation of the actual PAE scale, highlighting the limitations of self-reported data. Analysis using the cumulative biomarker index for two biomarkers with a threshold of ≥5 indicated that alcohol consumption affected approximately 3% of the studied population, further demonstrating the low reliability of maternal self-reports. Ultimately, this study confirms that the combined EtG and EtS measurements provide the most reliable diagnostic information for PAE and underscores the necessity of objective meconium screening in clinical practice. Full article

(This article belongs to the Section Drugs Toxicity)

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5 pages, 168 KB

Open AccessEditorial

Safe Utilization and Ecological Restoration of Heavy Metal Polluted Farmland: Latest Strategies for Remediation

by Bin Guo and Ying Feng

Toxics 2026, 14(2), 154; https://doi.org/10.3390/toxics14020154 - 3 Feb 2026

Abstract

Heavy metal pollution of farmland has emerged as a pressing global environmental challenge, which threatens food security, ecological integrity, and human health [...] Full article

(This article belongs to the Special Issue Safe Utilization and Ecological Restoration of Heavy Metal Polluted Farmland)

18 pages, 1321 KB

Open AccessArticle

Optimising Hydrocarbon Extraction from Soil Using Mixed-Surfactant Systems

by Emilio Ritoré, Carmen Arnaiz, José Morillo, Agata Egea-Corbacho and José Usero

Toxics 2026, 14(2), 153; https://doi.org/10.3390/toxics14020153 - 3 Feb 2026

Abstract

In industrial settings, one of the key environmental challenges is the remediation of soil contaminated by hydrocarbons. Washing the soil with surfactants mobilises and extracts these compounds, making them easier to treat. As it enables the recovery and reuse of soil within sustainable [...] Read more.

In industrial settings, one of the key environmental challenges is the remediation of soil contaminated by hydrocarbons. Washing the soil with surfactants mobilises and extracts these compounds, making them easier to treat. As it enables the recovery and reuse of soil within sustainable production processes, this technique is part of the circular economy. Soil-washing experiments using surfactants were carried out to determine whether a mixture of anionic and non-ionic surfactants could improve the remediation of soil contaminated by gasoline and diesel fuel compared to the use of a single surfactant. Four surfactants were used (non-ionic: polyoxyethylene lauryl ether and polyoxyethylene (80) sorbitan monooleate; anionic: sodium dodecylbenzenesulfonate and sodium dodecyl sulfate). The aliphatic and aromatic hydrocarbon fractions (C6–C8, C8–C10, C10–C12, C12–C16, C16–C21 and C21–C35) of gasoline and diesel fuel were analysed. Sodium dodecylbenzenesulfonate was selected for the purpose of preparing mixtures with the other two non-ionic surfactants, polyoxyethylene lauryl ether and polyoxyethylene (80) sorbitan monooleate. These surfactant mixtures demonstrated significantly higher removal rates than sodium dodecylbenzenesulfonate alone. Mixtures of sodium dodecylbenzenesulfonate and polyoxyethylene lauryl ether achieved hydrocarbon extraction of between 61% and 68%, while sodium dodecylbenzenesulfonate-polyoxyethylene (80) sorbitan monooleate mixtures obtained extraction of between 58% and 66%. Analysis of the gasoline and diesel hydrocarbon fractions indicated that smaller molecules desorb more easily than larger ones and that aromatics desorb more easily than aliphatics. Furthermore, the mixtures increased the extraction of both aliphatic and aromatic hydrocarbons, particularly the lighter compounds. The variation on removal rates within the hydrocarbon ranges may be related to the octanol–water partition coefficient (K_ow). These improvements with mixtures of anionic and non-ionic surfactants could be exploited to enhance the effectiveness of surfactant-flushing treatments and optimise the design of soil surfactant treatments. Full article

(This article belongs to the Special Issue Heavy Metals and Emerging Contaminants in Aquatic and Soil Environments: Migration, Transformation, Ecological Risks, and Remediation)

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26 pages, 12470 KB

Open AccessArticle

Read-Across Structural Analysis of PFAS Acute Oral Toxicity in Rats Powered by the Isalos Analytics Platform’s Automated Machine Learning

by Aikaterini Theodori, Konstantinos D. Papavasileiou, Andreas Tsoumanis, Georgia Melagraki and Antreas Afantitis

Toxics 2026, 14(2), 152; https://doi.org/10.3390/toxics14020152 - 3 Feb 2026

Abstract

The ubiquity and environmental persistence of per- and polyfluoroalkyl substances (PFASs) have raised significant concerns about their detrimental effects on human health. Collective scientific efforts are increasingly focused on elucidating PFAS toxicity mechanisms and identifying potential low-impact PFAS structures that retain the exceptional [...] Read more.

The ubiquity and environmental persistence of per- and polyfluoroalkyl substances (PFASs) have raised significant concerns about their detrimental effects on human health. Collective scientific efforts are increasingly focused on elucidating PFAS toxicity mechanisms and identifying potential low-impact PFAS structures that retain the exceptional properties of this chemical class. To advance the use of in silico methods in PFAS toxicity assessment, we developed a robust modelling framework for predicting PFAS acute oral toxicity class (high or low) in rats, leveraging the enhanced capabilities of the in-house Isalos Analytics Platform. The automated machine learning (autoML) functionality was employed to optimise four ML models—k-nearest neighbours (kNN), Random Forest (RF), eXtreme Gradient Boosting (XGBoost), and fully connected neural network (NN)—using Mold2 molecular descriptors, and to identify the top-performing model through five-fold cross-validation. The selected kNN model (k = 3) was used for predictions on the held-out testing set, achieving an accuracy of 81.5%, while a Shapley values analysis provided valuable insights into the factors influencing toxicity predictions. Furthermore, the nearest-neighbour-based methodology enabled a read-across structural analysis of PFAS similarity groups consisting of each testing set instance and its three closest neighbours in the training set. This analysis revealed a consistent association between polyaromatic and heterocyclic structural features and high acute oral toxicity. The developed, thoroughly validated read-across model is freely accessible through the INSIGHT RatTox web application as well as the INSIGHT Cheminformatics Platform in Enalos Cloud, supporting high-throughput screening of PFAS compounds and investigation of structural similarities with their nearest neighbours for enriched structural interpretation. Full article

(This article belongs to the Collection Predictive Toxicology)

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22 pages, 3529 KB

Open AccessArticle

Optimization of the Quantification of Antibiotic Resistance Genes in Media from the Yangtze River Estuary

by Jiadai Wu, Xinran Liu, Min Liu, Yawen Song, Qian Li, Jian Wang and Ye Huang

Toxics 2026, 14(2), 151; https://doi.org/10.3390/toxics14020151 - 2 Feb 2026

Abstract

Antibiotic resistance gene (ARG) monitoring in environmental systems increasingly relies on DNA-based molecular approaches; however, the extent to which DNA extraction strategies bias downstream resistome interpretation remains insufficiently understood. This study systematically evaluated the effects of single versus successive DNA extraction on DNA [...] Read more.

Antibiotic resistance gene (ARG) monitoring in environmental systems increasingly relies on DNA-based molecular approaches; however, the extent to which DNA extraction strategies bias downstream resistome interpretation remains insufficiently understood. This study systematically evaluated the effects of single versus successive DNA extraction on DNA recovery, microbial community composition, and the abundance and diversity of 385 genes related to antibiotic resistance including ARGs and mobile genetic elements (MGEs) across three contrasting matrices: water, sediment, and fish intestinal tissue. Successive extraction markedly increased DNA yield and detection of functional genes in water and sediment, particularly for low-abundance and particle-associated taxa. Enhanced recovery resulted in higher richness and abundance of ARGs and MGEs and strengthened correlations between intI1, ARGs, and bacterial taxa, indicating that single-cycle extraction may underestimate resistome magnitude and potential host associations in complex matrices. Conversely, fish intestinal tissue, used here as a representative biological matrix, showed limited benefit or even reduced gene abundance with repeated extraction, likely due to rapid depletion of extractable nucleic acids and DNA degradation. While successive extraction improves recovery efficiency, the potential inclusion of extracellular or relic DNA suggests caution in interpreting inflated ARG abundance. Overall, our findings demonstrate that DNA extraction is a matrix-dependent methodological driver that can reshape both quantitative outcomes and ecological inference. Matrix-specific optimization and careful protocol selection are therefore essential for improving data comparability and minimizing methodological underestimation in environmental resistome assessments. Full article

(This article belongs to the Special Issue Antibiotics and Resistance Genes in Environment)

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19 pages, 1142 KB

Open AccessArticle

Risk Assessment of Dibutyl Phthalate (DBP) and Bis(2-Ethylhexyl) Phthalate (DEHP) in Hot Pot Bases with a Hybrid Modeling Approach

by Xiangyu Bian, Siyu Huang, Dongya Chen, Depeng Jiang, Daoyuan Yang, Yingzi Zhao, Zhujun Liu, Shiqi Chen, Yan Song, Haixia Sui and Jinfang Sun

Toxics 2026, 14(2), 150; https://doi.org/10.3390/toxics14020150 - 2 Feb 2026

Abstract

(1) Background: Hot pot bases are susceptible to phthalate (PAE) contamination due to their high lipid content. Standard risk models often fail to capture extreme values, leading to biased exposure estimates. This study characterized dibutyl phthalate (DBP) and bis(2-ethylhexyl) phthalate (DEHP) contamination using [...] Read more.

(1) Background: Hot pot bases are susceptible to phthalate (PAE) contamination due to their high lipid content. Standard risk models often fail to capture extreme values, leading to biased exposure estimates. This study characterized dibutyl phthalate (DBP) and bis(2-ethylhexyl) phthalate (DEHP) contamination using a hybrid modeling framework to ensure precise risk profiling. (2) Methods: A total of 91 samples were analyzed via GC-MS. Concentration data were fitted using traditional parametric, extreme value mixture (EVMM), and finite mixture models. Probabilistic dietary risks were assessed for Chinese demographic groups using 10,000-iteration Monte Carlo simulations. (3) Results: DEHP (detection rate: 55%) and DBP (32%) were best modeled by a two-component Gamma mixture and a Lognormal–Generalized Pareto distribution, respectively. These advanced models significantly outperformed conventional distributions in capturing upper-tail extremes. Crucially, all hazard quotients (HQs) remained below the safety threshold of 1, indicating acceptable risk, although children aged 7–13 exhibited the highest calculated risk (Max DEHP HQ = 0.68). (4) Conclusions: Although current exposure levels are within safe limits, the heavy-tailed distributions identify potential sporadic high-exposure events that traditional models overlook, specifically highlighting the relative vulnerability of children aged 7–13. This study validates that hybrid statistical approaches offer superior precision for analyzing skewed contamination data. Consequently, these findings provide a critical scientific basis for refining regulatory monitoring and implementing targeted source-tracking measures to mitigate long-tail food safety risks. Full article

(This article belongs to the Special Issue Assessment of Dietary Exposure to Hazardous Chemicals and Health Risks)

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19 pages, 3631 KB

Open AccessArticle

Study on the Simultaneous Immobilization of Soluble Phosphorus and Fluorine in Phosphogypsum Using Activated Red Mud: Mechanism and Process Optimization

by Yi Wang, Yanhong Wang, Guohua Gu and Xuewen Wang

Toxics 2026, 14(2), 149; https://doi.org/10.3390/toxics14020149 - 2 Feb 2026

Abstract

Phosphogypsum (PG) is a byproduct of wet-process phosphoric acid production and contains soluble phosphorus (P), fluorine (F), and other harmful impurities in addition to calcium sulfate. Its acidic leachate enriched with P and F poses long-term risks to soil and surrounding water bodies. [...] Read more.

Phosphogypsum (PG) is a byproduct of wet-process phosphoric acid production and contains soluble phosphorus (P), fluorine (F), and other harmful impurities in addition to calcium sulfate. Its acidic leachate enriched with P and F poses long-term risks to soil and surrounding water bodies. Owing to the incorporation of soluble P and F within calcium sulfate crystal interlayers, these contaminants are gradually released during storage, making it difficult to achieve an economically efficient and environmentally benign treatment of PG at an industrial scale. In this study, a low-cost and sustainable process for the effective and long-term immobilization of soluble P and F in PG was developed using sulfuric acid-activated red mud (RM), an industrial waste rich in Fe and Al. After pulping PG with water, activated RM was added, followed by pH adjustment with Ca(OH)₂, leading to the in situ formation of amorphous calcium aluminate and calcium ferrite polymers with strong adsorption affinity toward soluble P and F. The immobilization mechanism and phase evolution were systematically investigated using inductively coupled plasma optical emission spectroscopy (ICP-OES, PS-6PLASMA SPECTROVAC, BAIRD, USA), on a Rigaku Miniflex diffractometer (Rigaku Corporation, Tokyo, Japan), scanning electron microscopy coupled with energy-dispersive spectroscopy (SEM-EDS), and zeta potential analysis. The leachate of PG treated with activated RM and Ca(OH)₂ contained P < 0.5 mg/L and F < 10 mg/L at pH 8.5–9.0, meeting environmental requirements (pH = 6–9, P ≤ 0.5 mg/L, F ≤ 10 mg/L). Moreover, the immobilized P and F exhibited enhanced stability during long-term stacking, indicating the formation of durable immobilization products. This study demonstrates an effective “treating waste with waste” strategy for the large-scale, environmentally safe utilization of phosphogypsum. Full article

(This article belongs to the Special Issue Multi-Media Environmental Pollution and Health Risks: Tracing, Effects, and Collaborative Governance)

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20 pages, 5520 KB

Open AccessFeature PaperArticle

Assessment of Per- and Poly-Fluoroalkyl Substances (PFAS) and Polybrominated Diphenyl Ethers (PBDEs) in Surface Waters Used for Urban Water Supply in Brazil

by Juliana de Souza-Araujo, Isadhora Camargo dos Santos, Hansel David Burgos Melo, Leila Soledade Lemos, Natalia Quinete and André Henrique Rosa

Toxics 2026, 14(2), 148; https://doi.org/10.3390/toxics14020148 - 2 Feb 2026

Abstract

This study assesses the presence and distribution of per- and polyfluoroalkyl substances (PFAS) and polybrominated diphenyl ethers (PBDEs) in the surface waters of the Itupararanga Reservoir and the Sorocaba River, Brazil. Samples collected during the dry and rainy seasons were analyzed to determine [...] Read more.

This study assesses the presence and distribution of per- and polyfluoroalkyl substances (PFAS) and polybrominated diphenyl ethers (PBDEs) in the surface waters of the Itupararanga Reservoir and the Sorocaba River, Brazil. Samples collected during the dry and rainy seasons were analyzed to determine their composition, spatial distribution, and seasonal variability. Results indicate the ubiquitous presence of PFAS, with significantly higher concentrations in the dry season, suggesting point sources of contamination, such as industrial and domestic discharges. Perfluorobutanoic acid (PFBA), Perfluorooctane sulfonate (PFOS), and Perfluorooctanoic acid (PFOA) were the predominant compounds, while 6:2 Fluorotelomer sulfonate (6-2FTS) stood out for its abundance in areas with industrial activity. For PBDEs, marked seasonal variability was observed, with higher concentrations during the rainy season, suggesting the mobilization of these compounds by surface runoff. BDE-209 was the most abundant congener, representing over 58% of the total concentration of PBDEs detected. Concentrations of PFAS and PBDEs in the study area are comparable to those reported globally, although there are differences associated with industrial practices and local environmental dynamics. The increased presence of short-chain PFAS and Deca-BDEs highlights the need for ongoing monitoring and the implementation of regulatory measures to mitigate contamination in water sources used for human consumption. Full article

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16 pages, 1468 KB

Open AccessArticle

Legacy and Emerging Organophosphate Esters (OPEs) in a Rural–Urban Transition Watershed: Spatiotemporal Distribution, Sources, and Toxicity Screening

by Shulin Guo, Weicong Deng, Xuan Zhan, Dan Li, Ivy Yik Fei Koo, Naisheng Zhang, Hongliang Chen, Qiabin Wang, Qin Liu, Xutao Wang, Yingxin Yu, Zenghua Qi and Yafeng Zhang

Toxics 2026, 14(2), 147; https://doi.org/10.3390/toxics14020147 - 1 Feb 2026

Abstract

Agricultural watersheds are undergoing rapid rural–urban transitions, yet the relative contributions of diffuse agricultural runoff versus rural domestic and point sources to organophosphate esters (OPEs) pollution remain poorly understood. This study investigated the occurrence, spatiotemporal distribution, and potential risks of 17 legacy and [...] Read more.

Agricultural watersheds are undergoing rapid rural–urban transitions, yet the relative contributions of diffuse agricultural runoff versus rural domestic and point sources to organophosphate esters (OPEs) pollution remain poorly understood. This study investigated the occurrence, spatiotemporal distribution, and potential risks of 17 legacy and emerging OPEs in the Dalongdong River, China. Combined non-target and target analyses revealed mean OPE concentrations of 111.94 ng/L in water and 8.76 ng/g in sediments. Spatially, total OPE concentrations increased progressively from upstream to downstream, with pronounced hotspots downstream of townships and near wastewater treatment facilities, indicating that rural domestic effluents and urban runoff, alongside agricultural activities, are critical contributors to OPE pollution in this watershed. Seasonally, concentrations of six legacy OPEs were significantly higher during the wet season. Furthermore, high-throughput phenotypic screening using Caenorhabditis elegans, combined with toxicological priority index analysis, showed that emerging OPEs generally pose higher integrated health and ecological risks, although certain legacy compounds, such as triphenyl phosphate, still display substantial toxic potential. These findings clarify the potential biological hazards of these compounds and provide baseline data on the fate of OPEs in riverine systems influenced by mixed agricultural and rural–urban anthropogenic activities. Full article

(This article belongs to the Special Issue Multi-Media Exposure to Emerging Contaminants and Health Effects Across Physiological Systems: Exposure Pathways, Risk Assessment and Public Health Implications)

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16 pages, 3708 KB

Open AccessArticle

Co-Exposure to Bisphenol A and a High-Fat Diet Induces Insulin Resistance via Suppression of Insulin Signaling Molecule Expression and GLUT4 Translocation

by Zeqi Lu, Min Cao, Jiaoxiang Zhang, Congzheng Qi, Bing Huang, Wenxue Li, Juntao Li, Guangyu Yang, Yan Zhang, Jinyin Wu, Weiwen Liu and Wei Zhu

Toxics 2026, 14(2), 146; https://doi.org/10.3390/toxics14020146 - 1 Feb 2026

Abstract

While the adverse health effects of bisphenol A (BPA) or high-fat diet (HFD) exposure alone have been relatively well documented, the mechanisms underlying their combined impact on insulin resistance and type 2 diabetes remain poorly understood. In this study, we observed the effects [...] Read more.

While the adverse health effects of bisphenol A (BPA) or high-fat diet (HFD) exposure alone have been relatively well documented, the mechanisms underlying their combined impact on insulin resistance and type 2 diabetes remain poorly understood. In this study, we observed the effects of 90 days of treatment with BPA and an HFD on insulin resistance in mouse gastrocnemius muscle, as well as the expression of signaling molecules and proteins potentially associated with glucose transporter type 4 (GLUT4) translocation. Additionally, C2C12 myotubes were co-treated with BPA and palmitic acid (PA) to observe the effects on insulin signaling molecules, GLUT4 translocation, and insulin resistance. Specifically, in vitro cellular experiments further demonstrated that BPA and PA inhibited GLUT4 translocation from the nucleus to the cell membrane. Taken together, co-exposure to BPA and an HFD (or PA) treatment significantly altered the expression of insulin signaling molecules in both gastrocnemius muscle and C2C12 cells, suggesting a potential link to their impacts on insulin resistance and GLUT4 translocation. Full article

(This article belongs to the Special Issue Health Risks and Toxicity of Emerging Contaminants)

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15 pages, 1892 KB

Open AccessArticle

Nanoceria’s Silent Threat: Investigating Acute and Sub-Chronic Effects of CeO₂ Nanopowder (≤50 nm) on the Human Intestinal Epithelial Cells

by Antonio Laganà, Angela Di Pietro, Caterina Saija, Maria Paola Bertuccio, Alessio Facciolà and Giuseppa Visalli

Toxics 2026, 14(2), 145; https://doi.org/10.3390/toxics14020145 - 1 Feb 2026

Abstract

The increased mobilization of Rare Earth Elements (REEs), due to emerging technologies, could impact human health. The study assessed the effects of CeO₂ nanopowder (100 μg/mL) in human intestinal cells (HT-29) following both acute (24 h) and, a novelty for in vitro [...] Read more.

The increased mobilization of Rare Earth Elements (REEs), due to emerging technologies, could impact human health. The study assessed the effects of CeO₂ nanopowder (100 μg/mL) in human intestinal cells (HT-29) following both acute (24 h) and, a novelty for in vitro study, sub-chronic exposure, treating subcultures of exposed cells to CeO₂ NP up to 35 days. Recovery was also examined in exposed cells’ progeny. CeO₂ NP internalization and acute cytotoxicity were dose and time dependent. A significant pro-oxidant effect was observed for up to 14 days. The highest mitochondrial impairment was detected after 7 days, but in post-exposure experiments the recovery was observed. Conversely, genotoxicity highlighted the saturation of the DNA repair mechanisms. The irreversible cell damage of sub-chronic exposure was highlighted by the percentage of death cells (p = 0.011) and by the weekly cell replication index (5.68 vs. 7.41). The homeostatic mitophagy pathway was able to counteract ROS-induced mitochondrial dysfunction, as shown by overexpression of ATG5, LC3, and BECN1 genes throughout the examined times. Instead, the overexpression of the pro-apoptotic gene Bax was very brief, highlighting that prolonged exposure might cause more widespread adverse effects, also involving cells that are not directly exposed to nanoceria. Full article

(This article belongs to the Special Issue Evaluation and Biomonitoring of Potentially Toxic and Rare Earth Elements)

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14 pages, 1414 KB

Open AccessArticle

Procoagulant Effects of Isothiazolinone Biocides, Benzisothiazolinone and Octylisothiazolinone in Platelets

by Ju Hee Choi and Keunyoung Kim

Toxics 2026, 14(2), 144; https://doi.org/10.3390/toxics14020144 - 1 Feb 2026

Abstract

Isothiazolinones are commonly used biocides that are extensively used in industrial areas and household products. The extensive usage of isothiazolinones raises concerns regarding their adverse human health effects. Isothiazolinones are readily absorbed and enter circulation. However, the potential systemic effects of isothiazolinones on [...] Read more.

Isothiazolinones are commonly used biocides that are extensively used in industrial areas and household products. The extensive usage of isothiazolinones raises concerns regarding their adverse human health effects. Isothiazolinones are readily absorbed and enter circulation. However, the potential systemic effects of isothiazolinones on the circulatory system remain unclear. Here, we examined whether the isothiazolinones, benzisothiazolinone (BIT) and octylisothiazolinone (OIT) affected platelets. In isolated platelets, BIT and OIT depleted intracellular glutathione, which led to mitochondrial reactive oxygen species (ROS) accumulation. Excessive mitochondrial ROS led to mitochondrial dysfunction, altering intracellular calcium and adenosine triphosphate homeostasis. These intracellular events activated phospholipid scramblase, externalizing phosphatidylserine, thereby enhancing procoagulant activity, as evidenced by thrombin generation. Overall, OIT showed a more potent effect than BIT. Notably, supplementation with N-acetyl-L-cysteine mitigated BIT- and OIT-induced effects, suggesting a thiol-dependent mechanism. Taken together, BIT and OIT stimulated the platelet-mediated coagulation pathway, which may increase prothrombotic risk and contribute to cardiovascular disease. These results could improve our understanding of the systemic adverse effects after isothiazolinone exposure. Full article

(This article belongs to the Section Emerging Contaminants)

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15 pages, 2068 KB

Open AccessArticle

Source Profile Analysis of Atmospheric Volatile Organic Compounds in Chongqing

by Anqi Zhang, Xin Qi, Yuchun Jiang, Hanfei Zuo, Yang Chen and Xiaoqian Li

Toxics 2026, 14(2), 143; https://doi.org/10.3390/toxics14020143 - 1 Feb 2026

Abstract

This study presents the first systematic investigation of Volatile Organic Compounds (VOC) source profiles from key industrial sectors in Chongqing, China. Source-specific emission data were collected from fifteen representative facilities encompassing furniture manufacturing, automobile production, and chemical industries through a combination of on-site [...] Read more.

This study presents the first systematic investigation of Volatile Organic Compounds (VOC) source profiles from key industrial sectors in Chongqing, China. Source-specific emission data were collected from fifteen representative facilities encompassing furniture manufacturing, automobile production, and chemical industries through a combination of on-site sampling and comprehensive literature review. Our results reveal distinct chemical signatures and regional variations among different source categories: furniture manufacturing emissions are dominated by alkanes (65%), chemical industries exhibit 51% alkane contribution, while automobile manufacturing demonstrates a remarkably high aromatic hydrocarbon content (64%), significantly exceeding other investigated sectors. Notably, aromatic hydrocarbons—particularly benzene derivatives—emitted from automotive manufacturing facilities pose potential carcinogenic and chronic health risks to both occupational workers and surrounding populations, necessitating prioritized regulatory intervention. These locally derived emission profiles fill a critical knowledge gap in regional VOC source characterization for Chongqing, providing essential scientific evidence for accurate source apportionment and formulation of sector-specific emission reduction strategies. Full article

(This article belongs to the Special Issue Analysis of Environmental Distribution, Mechanisms of Effects, and Risk of Pollutants Driven by New Technologies)

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15 pages, 2226 KB

Open AccessArticle

Histidine Metabolic Pathway Modifies the Relationships Between 6:2 Cl-PFESA Exposure and Preterm Birth

by Jianping Cong, Chu Chu, Zhitao Zhang, Gaoyuan Sun, Yan Zhang, Aaron M. Qian, Michael G. Vaughn, Sarah Dee Geiger, Kun Zhao, Yunting Zhang, Yang Zhou, Zhihua Yin and Guanghui Dong

Toxics 2026, 14(2), 142; https://doi.org/10.3390/toxics14020142 - 30 Jan 2026

Abstract

Background: Evidence linking chlorinated polyfluoroalkyl ether sulfonic acids (Cl-PFESAs) to preterm birth (PTB) is limited, and their relationships with the metabolome remain unexplored. Aims: Our study aimed to explore the role of the metabolome in the associations between Cl-PFESAs exposure and PTB. [...] Read more.

Background: Evidence linking chlorinated polyfluoroalkyl ether sulfonic acids (Cl-PFESAs) to preterm birth (PTB) is limited, and their relationships with the metabolome remain unexplored. Aims: Our study aimed to explore the role of the metabolome in the associations between Cl-PFESAs exposure and PTB. Methods: We conducted a nested case–control study in the Shenyang birth cohort, which included 206 spontaneous preterm birth cases and 206 full-term controls, matched for maternal age and pre-pregnancy BMI. We used conditional logistic regression models to analyze the associations between Cl-PFESAs exposure in umbilical cord blood and PTB. Moreover, the metabolomics of maternal blood (44 cases) between the preterm and control groups was analyzed using the interaction analysis. Results: We observed that a higher natural log-transformed 6:2 Cl-PFESA level was associated with greater odds of PTB in conditional multivariable-adjusted logistic regression models (OR = 1.738, 95% CI: 1.320, 2.287). The results of metabolomics pathway analysis showed that histidine metabolism pathways may modify the above risk. When stratified by histidine levels, the association between cord blood 6:2 Cl-PFESA and PTB was different. Conclusions: Intrauterine exposure to 6:2 Cl-PFESA was associated with increased PTB. Also, for the first time, our study illustrates that maternal plasma metabolite profiles may modify the associations of 6:2 Cl-PFESA with PTB. More research is needed to elucidate the mechanism underlying the reproductive toxicity of 6:2 Cl-PFESA in pregnant women following exposure. Full article

(This article belongs to the Special Issue Multi-Media Exposure to Emerging Contaminants and Health Effects Across Physiological Systems: Exposure Pathways, Risk Assessment and Public Health Implications)

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15 pages, 378 KB

Open AccessArticle

Phthalate Metabolites in Maternal Urine and Breast Milk After Very Preterm Birth: Matrix Concordance

by Esin Okman, Sıddika Songül Yalçın, Deniz Arca Çakır, Fuat Emre Canpolat, Suzan Yalçın and Pınar Erkekoğlu

Toxics 2026, 14(2), 141; https://doi.org/10.3390/toxics14020141 - 30 Jan 2026

Abstract

Background: Exposure to environmental pollutants, especially endocrine-disrupting chemicals, disproportionately affects vulnerable populations like pregnant women, lactating mothers, and preterm infants. This study aimed to assess the detection patterns of DiNP-, DEP-, and DEHP-related metabolites in maternal urine and breast milk, examine agreement between [...] Read more.

Background: Exposure to environmental pollutants, especially endocrine-disrupting chemicals, disproportionately affects vulnerable populations like pregnant women, lactating mothers, and preterm infants. This study aimed to assess the detection patterns of DiNP-, DEP-, and DEHP-related metabolites in maternal urine and breast milk, examine agreement between matrices, and explore maternal factors associated with phthalate exposure. Methods: Fifty-five mothers who delivered at ≤32 gestational weeks and whose infants were hospitalized in the Neonatal Intensive Care Unit (NICU) were enrolled. Breast milk and urine samples were analyzed using a validated isotope-dilution LC–MS/MS method. Urinary phthalate metabolite concentrations were adjusted for specific gravity. Linear mixed-effects models with a random intercept for mother were used to examine associations between urinary and breast milk phthalate metabolite concentrations, assess temporal changes, and evaluate the influence of breast milk lipid content. Results: DEHP and DiNP metabolites were detected in nearly all maternal urine samples. Breast milk contained predominantly primary metabolites (MEHP and MiNP), while secondary oxidative metabolites were rarely detected. Urine concentrations consistently exceeded breast milk concentrations. Urinary and breast milk phthalate concentrations were not correlated across sampling periods, indicating limited matrix concordance. Conclusions: Mothers of very preterm infants experience sustained phthalate exposure in the postpartum period; however, limited metabolite transfer to breast milk indicates that maternal urine remains the preferred biomonitoring matrix for assessing systemic phthalate exposure. Breast milk phthalate profiles exhibit compound-specific temporal changes and appear largely independent of concurrent urinary exposure biomarkers. Full article

(This article belongs to the Special Issue Toxicity of Phthalate Esters (PAEs))

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17 pages, 11145 KB

Open AccessArticle

Respirable α-Quartz Silica Triggers Immune–Inflammatory–Fibrotic Initiation in Zebrafish Embryos via Hindbrain Ventricle Microinjection: Implications for Silicosis Early Risk Assessment

by Linxuan Tian, Shen Yang, Xiaohong Liu, Junyan Tao and Lixin Yang

Toxics 2026, 14(2), 140; https://doi.org/10.3390/toxics14020140 - 30 Jan 2026

Abstract

Silicosis, an irreversible occupational lung disease resulting from prolonged exposure to respirable crystalline silica, faces challenges due to limitations in existing mammalian models. This study evaluated whether laboratory-prepared respirable α-quartz silica could induce immune cell–inflammatory–fibrotic initiation related to silicosis in zebrafish embryos as [...] Read more.

Silicosis, an irreversible occupational lung disease resulting from prolonged exposure to respirable crystalline silica, faces challenges due to limitations in existing mammalian models. This study evaluated whether laboratory-prepared respirable α-quartz silica could induce immune cell–inflammatory–fibrotic initiation related to silicosis in zebrafish embryos as a tool for early toxicity assessment. Zebrafish embryos at 48 h post-fertilization (hpf) were microinjected into hindbrain ventricle with respirable α-quartz silica (test material 3.056 μm vs. standard material 3.217 μm) derived from natural α-quartz ore. The results indicated a significant decrease in zebrafish survival rates and an increase in malformation rates following exposure respirable α-quartz silica materials. Additionally, alterations in midbrain and hindbrain lengths were observed, while body length remained unaffected. Behavioral assessments revealed reduced touch response rates, decreased average speed, and less time spent in the central zone during open field tests in the treatment groups. In vivo imaging demonstrated sequential recruitment of neutrophils (peak at 18 h post-injection) and macrophages (peak at 24 h post-injection). qPCR analysis revealed upregulation of inflammation-related genes (tnf-α, il-6, il-1β) and fibrosis-related genes (tgf-β, acta-2, collagen). Moreover, the hydroxyproline content, a marker for fibrosis, was significantly elevated, although no mature fibrosis was observed histologically. These findings demonstrate that respirable α-quartz silica elicits pathophysiological changes associated with silicosis early initiation in zebrafish embryos. This supports the utility of the zebrafish embryo as a practical tool for early toxicity assessment and mechanistic studies of silica-induced immune–inflammatory–fibrotic initiation, with potential implications for silica exposure early risk warning. Full article

(This article belongs to the Section Human Toxicology and Epidemiology)

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31 pages, 950 KB

Open AccessReview

Spatial Patterns of Breast Cancer Risk Associated with Industrial and Environmental Pollutants: A Scoping Review

by Darashagam Nahal, Abigail Hoffpauir, Kush Kinariwala, Priscilla Tetteh, Francesca Orenge, Anjali Patel, Ashreen Ghalib and Kari Northeim

Toxics 2026, 14(2), 139; https://doi.org/10.3390/toxics14020139 - 30 Jan 2026

Abstract

This scoping review examined published evidence linking environmental and industrial exposures to breast cancer, synthesizing studies conducted between 2015 and 2025. Using the Arksey and O’Malley framework, 51 peer-reviewed studies were identified and analyzed across five domains: study design, evidence quality, pollutant associations, [...] Read more.

This scoping review examined published evidence linking environmental and industrial exposures to breast cancer, synthesizing studies conducted between 2015 and 2025. Using the Arksey and O’Malley framework, 51 peer-reviewed studies were identified and analyzed across five domains: study design, evidence quality, pollutant associations, geographic emphasis, and research gaps. Most studies used retrospective designs, primarily case–control, ecological, cross-sectional, and cohort approaches, which identified associations but could not establish causation. Evidence of quality varied due to heterogeneous environmental modeling methods, exposure to misclassification concerns, and unmeasured confounding, even though 86 percent of studies had sample sizes larger than 1000 cases. Pesticides, polycyclic aromatic hydrocarbons (PAHs), and polychlorinated biphenyls (PCBs) were consistently associated with breast cancer, and nitrogen oxides (NOx), particulate matter (PM), and endocrine-disrupting chemicals (EDCs) also showed frequent significant associations. Research was geographically concentrated in North America and Europe, and few studies examined industrial hotspots or low-income regions. Gaps included the need for stronger epidemiological designs, multipollutant models, standardized exposure metrics, and clearer integration of significant environmental findings into public health protections. Overall, while evidence of pollution-related breast cancer risk continued to accumulate, the precautionary principle remained largely unimplemented. Advancing environmental policy, improving exposure transparency, and incorporating hotspot-based approaches are critical for reducing pollutant burdens and strengthening cancer prevention. Full article

(This article belongs to the Special Issue Health Effects of Exposure to Environmental Pollutants—2nd Edition)

34 pages, 837 KB

Open AccessFeature PaperReview

Cement Industry Pollution Mitigation: A Comprehensive Review on Reducing Environmental and Health Impacts

by Kamal Hosen and Alina Bărbulescu

Toxics 2026, 14(2), 138; https://doi.org/10.3390/toxics14020138 - 30 Jan 2026

Abstract

Cement production exerts a significant negative impact on the environment through the emission of greenhouse gases, particulate matter (PM), heavy metals, and other toxic substances into the atmosphere, soil, and bodies of water, degrading the environment and affecting the population’s health. This study [...] Read more.

Cement production exerts a significant negative impact on the environment through the emission of greenhouse gases, particulate matter (PM), heavy metals, and other toxic substances into the atmosphere, soil, and bodies of water, degrading the environment and affecting the population’s health. This study reviews different solutions to reduce pollution and mitigate its effects. Particular attention is given to Carbon Capture, Utilization, and Storage (CCUS) technologies and their ability to significantly reduce CO₂. Biomass and waste-derived fuels were identified as viable substitutes for fossil fuels, although challenges related to supply chain reliability and secondary environmental impacts remain. The study further examined mitigation strategies for non-gaseous pollutants, including noise pollution control measures such as sound barriers and vibration isolation systems, soil remediation techniques such as phytoremediation and the recycling of cement kiln dust (CKD), and water pollution control technologies, including filtration, chemical precipitation, biological treatment, and Zero Liquid Discharge (ZLD) systems. Key research gaps were identified, particularly concerning the long-term durability, scalability, and cost-effectiveness of these mitigation approaches. Overall, the review emphasizes the need for integrated pollution control strategies to support the transition toward a more sustainable cement industry and recommends future research focused on developing mitigation technologies that are efficient, economically viable, and adaptable to large-scale industrial applications. Full article

(This article belongs to the Special Issue Advances in Water, Air and Soil Pollution Monitoring, Modeling and Restoration, 2nd Edition)

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14 pages, 1171 KB

Open AccessArticle

Environmental Stress Shaping Oxidative Responses in the Invasive Crayfish Procambarus clarkii from Lake Trasimeno

by Barbara Caldaroni, Gianandrea La Porta, Ambrosius Josef Martin Dörr, Rebecca Gentile, Sara Futia, Alessandro Ludovisi, Matteo Pallottini, Roberta Selvaggi, Federica Bruschi and Antonia Concetta Elia

Toxics 2026, 14(2), 137; https://doi.org/10.3390/toxics14020137 - 30 Jan 2026

Abstract

Procambarus clarkii (red swamp crayfish) exhibits physiological plasticity that enables adaptation to variable freshwater conditions, such as those in Lake Trasimeno. This study examined whether fluctuations in hydrometric level and associated physicochemical parameters affect oxidative stress responses in the hepatopancreas and abdominal muscle [...] Read more.

Procambarus clarkii (red swamp crayfish) exhibits physiological plasticity that enables adaptation to variable freshwater conditions, such as those in Lake Trasimeno. This study examined whether fluctuations in hydrometric level and associated physicochemical parameters affect oxidative stress responses in the hepatopancreas and abdominal muscle of male and female individuals. Superoxide dismutase, catalase, glutathione peroxidase, and metallothionein reveal tissue, sex, and season-specific differences that indicate adaptive physiological adjustments. Temporal trends were evaluated, and multivariate analyses summarised environmental and metal gradients. Generalised Additive Models (GAMs) were used to explore relationships between oxidative responses and these gradients, with sex as a categorical factor. Associations were identified with hydrometric level, temperature, conductivity, transparency, pH, dissolved oxygen, and metals of biological relevance. These results highlight the remarkable physiological plasticity of P. clarkii, which underpins its success as an invasive species in fluctuating freshwater ecosystems. Full article

(This article belongs to the Section Ecotoxicology)

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