Extraction and Application Process of Bioactive Substances

A Special Issue of Processes (ISSN 2227-9717) belonging to the section "Pharmaceutical Processes".

Deadline for manuscript submissions: 15 September 2026 | Viewed by 1543

Editors


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Agro-Environmental Biotechnology Group, School of Chemistry, Autonomous University of Coahuila, Saltillo 25280, Coahuila, Mexico
Interests: biomolecules; bioprocessing; solid-state fermentation
Special Issues, Collections and Topics in MDPI journals

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Guest Editor
Laboratorio de Materiales Avanzados, Facultad de Ciencias Químicas, Universidad Autónoma de Coahuila, Coahuila, Saltillo 25280, Mexico
Interests: hydrogels; microencapsulation; nanoencapsulation; biological efficacy

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Instituto de Ciências Biológicas (ICB), Universidade de Pernambuco (UPE) Campus Santo Amaro, Recife 50100-130, Pernambuco, Brazil
Interests: Biochemistry; Biotechnology; Characterization; galactomannans
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

At present, increasingly diverse methods are needed to improve the quality of life in our population, and once again, we are turning our attention to natural options. A wide range of alternatives are available to us in plants and microbes that can be obtained and extracted without posing a threat to our environment. Biotechnological processing and green extraction methods for bioactive substances are being researched and developed to assess their application in areas such as food, feed, agriculture, biomedicine, biofuels, and bioenergy, among others.

By researching the biosynthetic potential of living tissues or microorganisms, biomolecules can be obtained, induced, produced, and recovered; many may also have significant roles in allelopathic interactions in agriculture, in the proliferation or antiproliferation of cell lines, or the (bio)processing of molecules for biotransformation into high-value-added products such as biofuels or biomaterial precursors. Several assays and tests can evaluate the beneficial effects of these molecules for a variety of purposes, leading to the development of innovative bioactive prototypes.

This Special Issue includes the following topics:

  • The extraction of biosynthetic products from plants and/or microorganisms;
  • Bioactivities in tests related to their application in areas such as food, feed, pharmaceutical, biomedical, agricultural, and biofuel production;
  • The encapsulation of bioactive compounds in hydrogels to enhance biological efficacy;
  • Conventional vs. emergent extraction technologies, bioaugmentation, biotransformation, enzymatic conversion, and other extraction strategies;
  • Experimental design, statistical optimization, and validation processes;
  • Encapsulation techniques, including microencapsulation and nanoencapsulation.

Dr. Miguel Á. Medina Morales
Dr. Jesús Alejandro Claudio-Rizo
Dr. Priscilla Barbosa Sales De Albuquerque
Guest Editors

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Keywords

  • biomolecules
  • bioprocessing
  • green processes
  • bioactivities
  • extraction strategies
  • biorefinery
  • hydrogel encapsulation
  • microencapsulation
  • nanoencapsulation
  • biological efficacy

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Published Papers (1 paper)

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Research

19 pages, 3375 KB  
Article
Bioactive Properties of Polyphenolic Extracts from Flourensia cernua Obtained by Emerging Technologies Under a Taguchi L18 Orthogonal Array
by Andrea G. Valero-Mendoza, Alberto Nuncio, Mayela Govea Salas, Alejandro Zugasti-Cruz, Leopoldo J. Ríos-González, Juan A. Ascacio-Valdés, Thelma K. Morales-Martínez, Marisol Cruz-Requena and Miguel A. Medina-Morales
Processes 2025, 13(11), 3725; https://doi.org/10.3390/pr13113725 - 18 Nov 2025
Cited by 1 | Viewed by 1120
Abstract
Flourensia cernua, commonly known as hojasén, is an endemic species from northern Mexico used in herbal medicine as a remedy for various stomach and respiratory ailments. In addition to its medicinal applications, this plant has notable antioxidant potential, making it a promising [...] Read more.
Flourensia cernua, commonly known as hojasén, is an endemic species from northern Mexico used in herbal medicine as a remedy for various stomach and respiratory ailments. In addition to its medicinal applications, this plant has notable antioxidant potential, making it a promising area of study. A crucial aspect in plant studies is the extraction method used, as conventional approaches can diminish the bioactivities present and affect the environment. This study aims to compare two sustainable extraction techniques, ultrasound and microwave-assisted (UAE/MAE), to maximize the yield of polyphenolic compounds from F. cernua. The Taguchi L18 orthogonal array was employed to evaluate total polyphenols and to examine independent variables, such as solvent concentration, temperature, and time. Additionally, the total flavonoid content and antioxidant activity were evaluated using the radicals ABTS●+ and DPPH, and the compounds were identified using RP-HPLC-ESI-MS. The results indicated that ultrasound showed better performance in recovering total bioactive compounds, correlating with antioxidant activity. Moreover, the in vitro, hemolytic, and antihemolytic assays demonstrated that F. cernua extracts are biocompatible and exhibit significant protective activity against oxidative damage in erythrocytes, supporting their potential cytoprotective and antioxidant properties. This suggests that ultrasound-assisted extraction (UAE) is an effective method for extracting phenolic compounds from F. cernua, with potential for optimizing conditions and facilitating biotechnological and therapeutic applications. Full article
(This article belongs to the Special Issue Extraction and Application Process of Bioactive Substances)
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