Genetic Engineering: Processes, Methods, Challenges and Solutions

A special issue of Processes (ISSN 2227-9717). This special issue belongs to the section "Biological Processes and Systems".

Deadline for manuscript submissions: 10 October 2025 | Viewed by 233

Special Issue Editors


E-Mail Website
Guest Editor
College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China
Interests: genetic engineering; genetic breeding of marine economic species; celluar & molecular genetics

E-Mail Website
Guest Editor
College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China
Interests: genomic selection; deep learning; genetics; image processing

Special Issue Information

Dear Colleagues,

Genetic engineering involves manipulating an organism's genetic material to achieve desired traits or functions. This Special Issue aims to provide a comprehensive overview of the processes, methods, challenges, and solutions in this field. A specific list of potential topics is outlined below.

Processes and Methods

  • DNA Extraction and Isolation: Extracting DNA from a source organism to isolate the gene of interest.
  • Gene Cloning and Design: Cloning the gene and tailoring it for expression in a different organism.
  • Transformation: Introducing the designed gene into the target organism's cells using methods like biolistics or Agrobacterium-mediated transformation.
  • Backcross Breeding: Combining desired traits through successive generations of crossing.

Applications

  • Agriculture: Developing pest-resistant and high-yielding crops.
  • Aquaculture: New methods in the genetic breeding of economically important marine and freshwater species.
  • Medicine: Producing recombinant proteins for therapeutic purposes.
  • Environmental Science: Using engineered microorganisms to degrade pollutants.

Challenges and Solutions

  • Ethical Concerns: Addressing ethical questions regarding GMOs and their long-term effects.
  • Unintended Consequences: Managing potential genetic variations and ecological disruptions.
  • Regulation and Safety: Ensuring rigorous testing and regulation for safe use.

Future Directions

Genetic engineering holds promise for addressing global challenges in agriculture, medicine, and environmental sustainability. This Special Issue invites researchers to submit original research papers, reviews, and perspectives on the processes, methods, challenges, and solutions in genetic engineering.

Dr. Yuxiang Liu
Prof. Dr. Yangfan Wang
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Processes is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • genetic engineering
  • DNA modification
  • gene cloning
  • transformation techniques
  • biotechnology
  • genetic editing

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Published Papers

This special issue is now open for submission.
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