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Applications of Plant Tissue Culture: New Insights into Plant Improvement...
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Applications of Plant Tissue Culture: New Insights into Plant Improvement Research Using Plant Tissue Culture and Plant Modification Techniques―2nd Edition

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Cell Biology".

Deadline for manuscript submissions: closed (31 December 2024) | Viewed by 4141

Special Issue Editors

Dr. David W. M. Leung

E-Mail Website
Guest Editor
School of Biological Sciences, University of Canterbury, Private Bag 4800, Christchurch 8140, New Zealand
Interests: plant biotechnology; in vitro plant breeding; micropropagation; phytoremediation; genetic modifications of plants; bio-printing
Special Issues, Collections and Topics in MDPI journals
Dr. Rambod Abiri

E-Mail Website
Guest Editor
Department of Plant Agriculture, University of Guelph, Guelph, ON N1G 2W1, Canada
Interests: plant genetics and biotechnology; plant-environment interactions; secondary metabolites; medicinal and aromatic plants; biotic and abiotic resistance
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The global population increase puts a huge pressure on the availability of food and water resources. Traditionally, plant breeders have played a key role in the genetic improvement of the quality and quantity of crops to feed the world’s growing population. Traditional plant breeding is, however, an ineffective method for sustained genetic improvement and has many limitations. The classical plant breeding procedure is time-consuming, reliant on limited natural genetic resources, and labour-intensive. Plant biotechnology has emerged as a reliable set of tools that empowers plant breeders to use genes of beneficial traits from diverse sources and insert them into plant genomes for genetic modifications. In addition, plant tissue culture (PTC) is a core biotechnology for maintaining or growing plant cells, tissues, or organs under aseptic conditions. There have been remarkable advances in PTC research on multiple fronts including the regulation of plant developmental processes in vitro, discoveries of biologically active compounds, gene transfer, and production of secondary metabolites of medicinal significance. Most recently, the integration of PTC with novel techniques such as genome editing has opened a new window for improving desirable traits in plants. Therefore, this Special Issue, titled “New Insight into Plant Improvement Research using Plant Tissue Culture and Plant Modification Techniques―2nd Edition”, aims to attract contributions on new advances in PTC, focusing on plant growth regulators, gene transformation, genome editing, molecular farming, and other applications of plant culture research in vitro. We welcome the submission of original research papers, reviews, and novel in vitro protocols including, but not limited to, the following topics:

  • Micropropagation;
  • Cell totipotency;
  • Phytochemical production by in vitro cultures;
  • Somaclonal variation;
  • Somatic embryogenesis;
  • De novo organogenesis;
  • Synthetic seeds;
  • Virus-free plants;
  • Cell suspension culture;
  • Hairy root culture;
  • Cellular reprogramming;
  • Callus formation;
  • Plant transformation;
  • Molecular plant breeding;
  • Genetics;
  • Genome editing;
  • DNA (transgene)-free editing;
  • Base/prime editing;
  • HDR-based editing;
  • The CRISPR/dCas9 system;
  • Off-target effects;
  • Transcription factors;
  • Rewiring of plant defence genes in vitro;
  • Bioreactors;
  • Secondary metabolites in plant tissue cultures;
  • Elicitors;
  • Plant defence mechanisms;
  • Biotic and abiotic stress in vitro culture;
  • Plant growth regulators;
  • Quality improvement;
  • Phytoremediation.

Dr. David W. M. Leung
Dr. Rambod Abiri
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Plants is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • micropropagation
  • molecular plant breeding
  • genome editing
  • biotic and abiotic stress in vitro culture
  • secondary metabolites in plant tissue cultures

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Related Special Issue

Published Papers (3 papers)

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Research

18 pages, 2367 KiB  
Article
Bottom Cooling During Culture Initiation Increases Survival and Reduces Hyperhydricity in Micropropagated Cannabis Plants
by Rambod Abiri, Declan O’Reilly and Andrew Maxwell Phineas Jones
Plants 2025, 14(6), 886; https://doi.org/10.3390/plants14060886 - 12 Mar 2025
Viewed by 956
Abstract
Hyperhydricity is characterized by morphological abnormalities and reduced plant vigour. This study investigated the use of a bottom cooling system (creating an approximate 2 °C temperature differential) during culture initiation to evaluate the impact on hyperhydricity in cannabis micropropagation. Nodal explants from two [...] Read more.
Hyperhydricity is characterized by morphological abnormalities and reduced plant vigour. This study investigated the use of a bottom cooling system (creating an approximate 2 °C temperature differential) during culture initiation to evaluate the impact on hyperhydricity in cannabis micropropagation. Nodal explants from two clonal triploid cultivars known to exhibit hyperhydricity, Higher Education 1 (HED-1) and Higher Education 2 (HED-2), were surface sterilized and placed in culture tubes using standard methods. Treatments included bottom cooling, metal pads without bottom cooling, and standard shelving (controls—no pad). Various morphological and physiological traits were assessed, including a detached leave water loss assay, dry mass, chlorophyll content, and survival rate. Plants cultured with bottom cooling showed significantly higher survival rates, healthier appearance, and improved physiological parameters compared to controls. In contrast, many control explants were hyperhydric with translucent and brittle leaves. Quantitative data revealed significant improvements in fresh weight (54.84% for HED-1 and 51.42% for HED-2), dry weight (36% for HED-1 and 8% for HED-2), chlorophyll fluorescence ratios (7.24% for HED-1 and 9.18% for HED-2), chlorophyll content (18.38% for HED-1 and 20.67% for HED-2), and cuticle/stomate function (30% for HED-1 and 27.27% for HED-2) using bottom cooling. Moreover, our morphological observation showed that almost 85% of control plants were hyperhydric, whereas only 10% of the plants cultured with a bottom cooling system were hyperhydric. This study confirmed that bottom cooling helps reduce the rate and impacts of hyperhydricity in cannabis and significantly improves the survival and quality of in vitro plants. Full article
(This article belongs to the Special Issue Applications of Plant Tissue Culture: New Insights into Plant Improvement Research Using Plant Tissue Culture and Plant Modification Techniques―2nd Edition)
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12 pages, 1581 KiB  
Article
Influence of Sucrose and Immersion Time on Humulus lupulus L., cv Columbus, Plantlet In Vitro Proliferation and Potentially Bioactive Compound Content
by Valeria Gianguzzi, Leandra Leto, Anna Agosti, Andrea Di Fazio, Francesco Paolo Marra, Martina Cirlini and Benedetta Chiancone
Plants 2025, 14(4), 537; https://doi.org/10.3390/plants14040537 - 10 Feb 2025
Cited by 1 | Viewed by 747
Abstract
(1) Background: Traditionally, hop is propagated using rhizome fragments or herbaceous stem cuttings. Micropropagation, therefore, offers a viable alternative for the large-scale production of healthy, genetically uniform plants, regardless of the season and within confined spaces. A temporary immersion system (TIS) facilitates plant [...] Read more.
(1) Background: Traditionally, hop is propagated using rhizome fragments or herbaceous stem cuttings. Micropropagation, therefore, offers a viable alternative for the large-scale production of healthy, genetically uniform plants, regardless of the season and within confined spaces. A temporary immersion system (TIS) facilitates plant propagation by alternating immersions of microcuttings in liquid culture medium with dry periods, preventing gas accumulation through forced ventilation. (2) Methods: In this study, the response of hop plantlets, cv. Columbus, cultured in media with and without sucrose, in solid and liquid culture systems (PlantformTM bioreactor), was evaluated, considering its effect on morpho-physiological parameters, on the total phenolic content, and on antioxidant capacity. Moreover, to make the TIS more efficient, the effect of immersion duration (three and six minutes every twelve hours) was evaluated. (3) Results: The presence of sucrose in the culture medium improved plant proliferation in both culture systems tested: solid and liquid (particularly for explants immersed for three minutes). In the TIS, plantlets with a higher antioxidant capacity were obtained when sucrose-free culture medium was used. (4) Conclusions: This study confirms the efficacy of the TIS as a hop propagation method but also as a valid tool to produce biomass to be used as a source of bioactive compounds. Full article
(This article belongs to the Special Issue Applications of Plant Tissue Culture: New Insights into Plant Improvement Research Using Plant Tissue Culture and Plant Modification Techniques―2nd Edition)
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18 pages, 9678 KiB  
Article
Characteristics of Callus and Cell Suspension Cultures of Highbush Blueberry (Vaccinium corymbosum L.) Cultivated in the Presence of Different Concentrations of 2,4-D and BAP in a Nutrient Medium
by Dmitry A. Rybin, Alina A. Sukhova, Andrey A. Syomin, Tatiana A. Zdobnova, Ekaterina V. Berezina and Anna A. Brilkina
Plants 2024, 13(23), 3279; https://doi.org/10.3390/plants13233279 - 22 Nov 2024
Cited by 3 | Viewed by 1407
Abstract
In this work, cultures of callus and suspension cells originating from leaves of sterile highbush blueberry (Vaccinium corymbosum L.) plants were obtained and characterized. For their active growth and production of phenolic compounds, a combination of 2,4-D at a concentration of 0.34–2.25 [...] Read more.
In this work, cultures of callus and suspension cells originating from leaves of sterile highbush blueberry (Vaccinium corymbosum L.) plants were obtained and characterized. For their active growth and production of phenolic compounds, a combination of 2,4-D at a concentration of 0.34–2.25 µM and BAP at a concentration of 0.45–2.25 µM is effective. An increase in the phytohormone concentration leads to a slowdown in culture formation and reduces their ability to synthesize phenolic compounds. When cultivating V. corymbosum suspension cells over a year (12 passages), they not only retain the ability to synthesize phenolic compounds but also enhance it. By the 12th passage, the content of TSPC in suspension cells reaches 150 mg/g DW, the content of flavonoids reaches 100 mg/g DW, the content of flavans reaches 40 mg/g DW, and the content of proanthocyanidins reaches 30 mg/g DW. The high content of phenolic compounds may be due to the high expression of genes in flavonoid biosynthesis enzymes. V. corymbosum suspension cells accumulate a high level of phenolic compounds during a passage. The ability of V. corymbosum callus and cell suspension cultures in the presence of low concentrations of phytohormones to grow and accumulate biologically active phenolic compounds determines their high economic significance and prospects for organizing a biotechnological method for obtaining phenolic compounds. Full article
(This article belongs to the Special Issue Applications of Plant Tissue Culture: New Insights into Plant Improvement Research Using Plant Tissue Culture and Plant Modification Techniques―2nd Edition)
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