Biosensors Based on Recombinant Antibodies—in Memory of Professor Hiroshi Ueda

A special issue of Methods and Protocols (ISSN 2409-9279). This special issue belongs to the section "Biochemical and Chemical Analysis & Synthesis".

Deadline for manuscript submissions: closed (20 May 2024) | Viewed by 1822

Special Issue Editors


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Guest Editor
Department of Chemistry and Bioscience, Aalborg University, Aalborg, Denmark
Interests: molecular evolution; phage display; recombinant antibody technology; enzyme engineering; cellular model; proteomics; cancer; neurodegenerative diseases; infectious diseases
Special Issues, Collections and Topics in MDPI journals

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Guest Editor
1. School of Rehabilitation Sciences and Engineering, University of Health and Rehabilitation Sciences, Qingdao, China
2. Institute of Innovative Research, Tokyo Institute of Technology, Yokohama, Japan
Interests: antibody engineering; the principles and applications of immunoassay and diagnosis; antibody medicines; enzyme artificial evolution
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Immunosensors are a kind of biosensor that is based on the specific recognition function of antigens and antibodies. Due to the advantages of high sensitivity, high specificity, simple use and low cost, immunosensor technology has been applied in a wide range of fields such as clinical medicine and biological monitoring technology, food industry, environmental monitoring and processing. With the development of molecular biology, the preparation of antibody fragments has become easier, and the immunodetection technology based on recombinant antibody fragments has also rapidly developed. Professor Hiroshi Ueda of the Tokyo Institute of Technology in Japan has experienced many great achievements in the research of biosensors based on antibody fragments. The Open Sandwich Immunoassay and Quenchbody technology invented by Professor Ueda have greatly contributed to the field. To commemorate the outstanding contributions of Professor Ueda, this Special Issue will mainly collect papers on detection technology based on antibody fragments to promote innovation and development in this field.

You may choose our Joint Special Issue in Sensors.

Dr. Peter Kristensen
Prof. Dr. Jinhua Dong
Guest Editors

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Keywords

  • antibody
  • alternative scaffolds
  • immunoassay
  • diagnosis
  • fluorescent
  • biomarker
  • phage display

Published Papers (1 paper)

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9 pages, 1190 KiB  
Technical Note
Generation of a Recombinant scFv against Deoxycholic Acid and Its Conversion to a Quenchbody for One-Step Immunoassay
by Hiroshi Ueda and Hee-Jin Jeong
Methods Protoc. 2023, 6(5), 90; https://doi.org/10.3390/mps6050090 - 30 Sep 2023
Cited by 1 | Viewed by 1369
Abstract
Development of a rapid detection method for deoxycholic acid (DCA) is crucial for its diagnosis in the early stages of inflammation and cancer. In this study, we expressed a soluble recombinant anti-DCA single-chain variable fragment (scFv) in Escherichia coli. To convert scFv [...] Read more.
Development of a rapid detection method for deoxycholic acid (DCA) is crucial for its diagnosis in the early stages of inflammation and cancer. In this study, we expressed a soluble recombinant anti-DCA single-chain variable fragment (scFv) in Escherichia coli. To convert scFv into a Quenchbody (Q-body), we labeled scFv using commercially available maleimide-linked fluorophores. The TAMRA-C5-maleimide-conjugated Q-body showed the highest response within a few minutes of DCA addition, indicating its applicability as a wash-free immunoassay probe for onsite DCA detection. Full article
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