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Synthesis of Natural Products Using Engineered Plants and Microorganisms, 2nd Edition

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Natural Products Chemistry".

Deadline for manuscript submissions: 31 July 2025 | Viewed by 1415

Special Issue Editors


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Guest Editor
School of Pharmaceutical Sciences, Zhengzhou University, Zheng Zhou 450001, China
Interests: traditional medicinal plants; synthetic biology; chemical biology; food nutrition; pharmaceutical molecules
Special Issues, Collections and Topics in MDPI journals

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Guest Editor
College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing 211816, China
Interests: metabolic engineering; synthetic biology; oleaginous yeast; Yarrowia lipolytica; oleochemicals; terpenoids
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Plants and microorganisms, particularly medicinal herbs, harbor diverse natural products, many of which are bioactive molecules with potential pharmaceutical or health benefits. However, the compositions of these bioactive molecules in their original sources are usually low. The development of omics technologies and synthetic biology provide opportunities to produce bioactive molecules using metabolically engineered plants or microorganisms. Artemisinin, rare ginsenosides, and various other natural products have been successfully scaled up for potential use as drugs or functional foods. There is a growing interest in further harnessing cutting-edge synthetic biology technologies to expand the utilization of these natural products via engineering both plants and microorganisms.

Dr. Yongjun Wei
Prof. Dr. Lingbo Qu
Prof. Dr. Xiao-Jun Ji
Guest Editors

Manuscript Submission Information

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Keywords

  • natural products
  • bioactive molecules
  • medicinal herbs
  • metabolic engineering
  • microbial cell factories
  • synthetic biology
  • natural product biosynthetic pathway
  • omics technologies
  • large-scale production pathway optimization

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Published Papers (1 paper)

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Research

12 pages, 3477 KiB  
Article
Efficient Biotransformation of Icariin to Baohuoside I Using Two Novel GH1 β-Glucosidases
by Xiaoling Zhang, Yitong Wang, Tiantian Zhang, Ziqiao Yuan and Yongjun Wei
Molecules 2024, 29(22), 5280; https://doi.org/10.3390/molecules29225280 - 8 Nov 2024
Viewed by 987
Abstract
Epimedium Folium (EF) is a traditional Chinese herbal medicine, and its primary bioactive ingredients, such as icariin, are flavonoid glycosides. A rare EF flavonoid, baohuoside I, exhibits superior bioactivities and enhanced bioavailability compared to its metabolic precursor icariin. The biotransformation of icariin to [...] Read more.
Epimedium Folium (EF) is a traditional Chinese herbal medicine, and its primary bioactive ingredients, such as icariin, are flavonoid glycosides. A rare EF flavonoid, baohuoside I, exhibits superior bioactivities and enhanced bioavailability compared to its metabolic precursor icariin. The biotransformation of icariin to baohuoside I can be effectively and specifically achieved by β-glucosidases. In this study, 33 candidate full-length β-glucosidase genes were screened from a previously built carbohydrate active enzyme (CAZyme) gene dataset derived from cow fecal microbiota. Thirteen of them exhibited β-glucosidase activity, with DCF-bgl-26 and DCF-bgl-27 showing relatively high expression levels and β-glucosidase activity. The maximum β-glucosidase activity of DCF-bgl-26 and DCF-bgl-27 was achieved at 45 °C and pH 6.0, with DCF-bgl-26 demonstrating better thermostability and pH tolerance compared to DCF-bgl-27. The activities of DCF-bgl-26 and DCF-bgl-27 were 123.2 U/mg protein and 157.9 U/mg protein, respectively, both of which are higher than those of many bacterial β-glucosidases. Structure analysis suggested that both β-glucosidases possess canonical (β/α)8-TIM barrel fold structure of GH1 family β-glucosidases. Thin-layer chromatography results showed that both enzymes could efficiently convert icariin to baohuoside I in 30 min, indicating they have potential application in the production of high value rare baohuoside I. Full article
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