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Microorganisms
Special Issues
Advances in Microbial Surfactants: Production and Applications (2nd Edition)
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Advances in Microbial Surfactants: Production and Applications (2nd Edition)

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Microbial Biotechnology".

Deadline for manuscript submissions: 31 October 2025 | Viewed by 457

Special Issue Editors

Prof. Dr. Marcia Nitschke

E-Mail Website
Guest Editor
São Carlos Institute of Chemistry (IQSC), University of São Paulo, Trabalhador São-Carlense Av., 400, P.O. Box 780, São Carlos 13560-970, SP, Brazil
Interests: biosurfactants; biofilms
Special Issues, Collections and Topics in MDPI journals
Dr. Xanel Vecino

E-Mail Website
Guest Editor
Research Center in Technologies, Energy and Industrial Processes (CINTECX), Chemical Engineering Department, Campus As Lagoas-Marcosende, University of Vigo, 36310 Vigo, Spain
Interests: natural products; antioxidants; membrane technology; bioremediation; wastewater treatment; lactic acid bacteria; biosurfactants; cosmetic; agroindustrial wastes; bioadsorbents; biotechnology process
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

This Special Issue is the continuation of our 2023 Special Issue “Advances in Microbial Surfactants: Production and Applications”.

The increasing demand for a more sustainable society motivates the search for green and eco-friendly alternatives to chemical-derived compounds. Microbial surfactants are potential candidates to replace synthetics, especially due to their biological origin and production from renewable resources. In addition, the interesting properties demonstrated by biosurfactants are useful for a range of industrial applications, from environmental to pharmaceutical.  Due to their great metabolic versatility, bacteria are the most traditional surfactant producers; however, filamentous fungi, yeasts, algae, and extremophile bacteria and archaea are under-exploited. In this Special Issue, we invite authors to submit original articles focusing on (but not limited to) the following research topics: characterization of surfactants synthesized by novel strains, production of biosurfactants from alternative substrates emphasizing circular bioeconomy, and biological activity and applications of microbial surfactants in the health, agriculture, food, environmental, and nanotechnology fields.

Prof. Dr. Marcia Nitschke
Dr. Xanel Vecino
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Microorganisms is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • biosurfactants
  • agroindustrial wastes
  • antimicrobial activity
  • bioremediation
  • biofilm
  • nanotechnology
  • glycolipid
  • lipopeptide

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Published Papers (1 paper)

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Research

24 pages, 2320 KiB  
Article
Glucoselipid Biosurfactant Biosynthesis Operon of Rouxiella badensis DSM 100043T: Screening, Identification, and Heterologous Expression in Escherichia coli
by Andre Fahriz Perdana Harahap, Chantal Treinen, Leonardo Joaquim Van Zyl, Wesley Trevor Williams, Jürgen Conrad, Jens Pfannstiel, Iris Klaiber, Jakob Grether, Eric Hiller, Maliheh Vahidinasab, Elvio Henrique Benatto Perino, Lars Lilge, Anita Burger, Marla Trindade and Rudolf Hausmann
Microorganisms 2025, 13(7), 1664; https://doi.org/10.3390/microorganisms13071664 - 15 Jul 2025
Viewed by 279
Abstract
Rouxiella badensis DSM 100043T had been previously proven to produce a novel glucoselipid biosurfactant which has a very low critical micelle concentration (CMC) as well as very good stability against a wide range of pH, temperature, and salinity. In this study, we [...] Read more.
Rouxiella badensis DSM 100043T had been previously proven to produce a novel glucoselipid biosurfactant which has a very low critical micelle concentration (CMC) as well as very good stability against a wide range of pH, temperature, and salinity. In this study, we performed a function-based library screening from a R. badensis DSM 100043T genome library to identify responsible genes for biosynthesis of this glucoselipid. The identified open reading frames (ORFs) were cloned into several constructs in Escherichia coli for gene permutation analysis and the individual products were analyzed using high-performance thin-layer chromatography (HPTLC). Products of interest from positive expression strains were purified and analyzed by liquid chromatography/electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) and nuclear magnetic resonance (NMR) for further structure elucidation. Function-based screening of 5400 clones led to the identification of an operon containing three ORFs encoding acetyltransferase GlcA (ORF1), acyltransferase GlcB (ORF2), and phosphatase/HAD GlcC (ORF3). E. coli pCAT2, with all three ORFs, resulted in the production of identical R. badensis DSM 100043T glucosedilipid with Glu-C10:0-C12:1 as the main congener. ORF2-deletion strain E. coli pAFP1 primarily produced glucosemonolipids, with Glu-C10:0,3OH and Glu-C12:0 as the major congeners, predominantly esterified at the C-2 position of the glucose moiety. Furthermore, fed-batch bioreactor cultivation of E. coli pCAT2 using glucose as the carbon source yielded a maximum glucosedilipid titer of 2.34 g/L after 25 h of fermentation, which is 55-fold higher than that produced by batch cultivation of R. badensis DSM 100043T in the previous study. Full article
(This article belongs to the Special Issue Advances in Microbial Surfactants: Production and Applications (2nd Edition))
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