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Micromachines
Special Issues
Tissue Engineering and Regenerative Medicine with Micromachines
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Tissue Engineering and Regenerative Medicine with Micromachines

A special issue of Micromachines (ISSN 2072-666X). This special issue belongs to the section "B：Biology and Biomedicine".

Deadline for manuscript submissions: closed (31 July 2023) | Viewed by 3711

Special Issue Editors

Prof. Dr. Patricia Pranke

E-Mail Website
Guest Editor
Hematology and Stem Cell Laboratory, Faculty of Pharmacy, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 90610-000, Brazil
Interests: stem cells; biomaterials; nanotechnology; tissue engineering and regenerative medicine
Dr. Laura Elena Sperling

E-Mail Website
Guest Editor
School of Health Sciences, Faculty of Medicine, UNISINOS, São Leopoldo 93022-750, Brazil
Interests: stem cells, cells and biomaterials in the treatment of spinal cord injury; nanotechnology; peripheral nerve regeneration and tissue engineering
Dr. Natasha Maurmann

E-Mail Website
Guest Editor
Hematology and Stem Cell Laboratory, Faculty of Pharmacy, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 90.610-000, Brazil
Interests: stem cells; biomaterials; nanotechnology; pharmacy; tissue engineering; regenerative medicine
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Tissue engineering and regenerative medicine include research on therapeutic applications to repair, replace, restore, regenerate, or ameliorate tissue and organ function. This innovative area has evolved tremendously over the past few years, its aim being to mimic native tissue, and it remains a field of knowledge with significant promise for transforming healthcare and quality of life.

The Special Issue, "Tissue Engineering and Regenerative Medicine with Micromachines", focuses on studying the effects of cells, growth factors, and/or biomedical materials to improve the development of tissue engineering and regenerative medicine. The purpose of this Special Issue is to publish cutting-edge applications related to advanced biomaterials for cell culture, including drug delivery and in vivo tests. A large variety of materials, either synthetic, natural or composites and cell types, such as cell lines, primary cell culture, and stem/stromal cells (such as induced pluripotent stem cells, mesenchymal stem cells, and adult stem cells), are welcome. We are also interested in articles presenting works with electrospinning, bioprinting, additive manufacturing, organ-on-a-chip, and bioreactors.

Prof. Dr. Patricia Pranke
Dr. Laura Elena Sperling
Dr. Natasha Maurmann
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Micromachines is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • biomaterials
  • biomedical engineering
  • bioprinting
  • cellular, tissue and genetic engineering
  • nanostructures
  • organ-on-a-chip
  • organ regeneration
  • primary cells
  • stem/stromal cells
  • scaffolds

Published Papers (3 papers)

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Research

19 pages, 4518 KiB  
Article
3D Artificial Skin Platform for Investigating Pregnancy-Related Skin Pigmentation
by Uiechan Jeong, Sunhee Yoon, Sungjin Park, Tae-Joon Jeon and Sun Min Kim
Micromachines 2024, 15(4), 511; https://doi.org/10.3390/mi15040511 - 10 Apr 2024
Viewed by 660
Abstract
In this study, we created a 3D Artificial Skin Platform that can be used for the treatment of pigmentation by artificially realizing the skin of pregnant women. For the stable realization of 3D artificial skin, a bilayer hydrogel composed of collagen type I [...] Read more.
In this study, we created a 3D Artificial Skin Platform that can be used for the treatment of pigmentation by artificially realizing the skin of pregnant women. For the stable realization of 3D artificial skin, a bilayer hydrogel composed of collagen type I and fibrin was designed and applied to the study to reduce the tension-induced contraction of collagen type I, the extracellular matrix (ECM) of artificial skin, by dynamic culture. Oxygen concentration and 17β-Estradiol (E2) concentration, which are highly related to melanin production, were selected as parameters of the pregnancy environment and applied to cell culture. Oxygen concentration, which is locally reduced in the first trimester (2.5–3%), and E2, which is upregulated in the third trimester, were applied to the cell culture process. We analyzed whether the 3D artificial skin implemented in the 3D Artificial Skin Platform could better represent the tendency of melanin expression in pregnant women than cells cultured under the same conditions in 2D. The expression levels of melanin and melanin-related genes in the 2D cell culture did not show a significant trend that was similar to the melanin expression trend in pregnant women. However, the 3D artificial skin platform showed a significant trend towards a 2-6-fold increase in melanin expression in response to low oxygen concentrations (2.5%) and E2 concentrations (17 ng/mL), which was similar to the trend in pregnant women in vivo. These results suggest that 3D artificial skin cultured on the Artificial Skin Platform has the potential to be used as a substitute for human pregnant skin in various research fields related to the treatment of pigmentation. Full article
(This article belongs to the Special Issue Tissue Engineering and Regenerative Medicine with Micromachines)
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30 pages, 7153 KiB  
Article
Improving Pure Titanium’s Biological and Mechanical Characteristics through ECAP and Micro-Arc Oxidation Processes
by Dawit Bogale Alemayehu, Masahiro Todoh, Jang-Hsing Hsieh, Chuan Li and Song-Jeng Huang
Micromachines 2023, 14(8), 1541; https://doi.org/10.3390/mi14081541 - 31 Jul 2023
Cited by 1 | Viewed by 1161
Abstract
Pure titanium is limited to be used in biomedical applications due to its lower mechanical strength compared to its alloy counterpart. To enhance its properties and improve medical implants feasibility, advancements in titanium processing technologies are necessary. One such technique is equal-channel angular [...] Read more.
Pure titanium is limited to be used in biomedical applications due to its lower mechanical strength compared to its alloy counterpart. To enhance its properties and improve medical implants feasibility, advancements in titanium processing technologies are necessary. One such technique is equal-channel angular pressing (ECAP) for its severe plastic deformation (SPD). This study aims to surface modify commercially pure titanium using micro-arc oxidation (MAO) or plasma electrolytic oxidation (PEO) technologies, and mineral solutions containing Ca and P. The composition, metallography, and shape of the changed surface were characterized using X-ray diffraction (XRD), digital optical microscopy (OM), and scanning electron microscope (SEM), respectively. A microhardness test is conducted to assess each sample’s mechanical strength. The weight % of Ca and P in the coating was determined using energy dispersive spectroscopy (EDS), and the corrosion resistance was evaluated through potentiodynamic measurement. The behavior of human dental pulp cell and periodontal cell behavior was also studied through a biomedical experiment over a period of 1-, 3-, and 7-days using culture medium, and the cell death and viability can be inferred with the help of enzyme-linked immunosorbent assay (ELISA) since it can detect proteins or biomarkers secreted by cells undergoing apoptosis or necrosis. This study shows that the mechanical grain refinement method and surface modification might improve the mechanical and biomechanical properties of commercially pure (CP) titanium. According to the results of the corrosion loss measurements, 2PassMAO had the lowest corrosion rate, which is determined to be 0.495 mmpy. The electrode potentials for the 1-pass and 2-pass coated samples are 1.44 V and 1.47 V, respectively. This suggests that the coating is highly effective in reducing the corrosion rate of the metallic CP Ti sample. Changes in the grain size and the presence of a high number of grain boundaries have a significant impact on the corrosion resistance of CP Ti. For ECAPED and surface-modified titanium samples in a 3.6% NaCl electrolyte solution, electrochemical impedance spectroscopy (EIS) properties are similar to Nyquist and Bode plot fitting. In light of ISO 10993-5 guidelines for assessing in vitro cytotoxicity, this study contributes valuable insights into pulp and periodontal cell behavior, focusing specifically on material cytotoxicity, a critical factor determined by a 30% decrease in cell viability. Full article
(This article belongs to the Special Issue Tissue Engineering and Regenerative Medicine with Micromachines)
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14 pages, 2451 KiB  
Article
Easy-to-Assembly System for Decellularization and Recellularization of Liver Grafts in a Bioreactor
by Maurício Felisberto Borges, Natasha Maurmann and Patricia Pranke
Micromachines 2023, 14(2), 449; https://doi.org/10.3390/mi14020449 - 14 Feb 2023
Cited by 2 | Viewed by 1443
Abstract
Decellularization of organs creates an acellular scaffold, ideal for being repopulated by cells. In this work, a low-cost perfusion system was created to be used in the process of liver decellularization and as a bioreactor after recellularization. It consists of a glass chamber [...] Read more.
Decellularization of organs creates an acellular scaffold, ideal for being repopulated by cells. In this work, a low-cost perfusion system was created to be used in the process of liver decellularization and as a bioreactor after recellularization. It consists of a glass chamber to house the organ coupled to a peristaltic pump to promote liquid flow through the organ vascular tree. The rats’ liver decellularization was made with a solution of sodium dodecyl sulfate. The recellularization was made with 108 mesenchymal stromal/stem cells and cultivated for seven days. The decellularized matrices showed an absence of DNA while preserving the collagen and glycosaminoglycans quantities, confirming the efficiency of the process. The functional analyses showed a rise in lactate dehydrogenase levels occurring in the first days of the cultivation, suggesting that there is cell death in this period, which stabilized on the seventh day. Histological analysis showed conservation of the collagen web and some groups of cells next to the vessels. It was possible to establish a system for decellularization and a bioreactor to use for the recellularization method. It is easy to assemble, can be ready to use in little time and be easily sterilized. Full article
(This article belongs to the Special Issue Tissue Engineering and Regenerative Medicine with Micromachines)
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