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JSOPB 2022–2023 Congress Special Issue – Update on Organ Preservation and Transplantation

A special issue of Journal of Clinical Medicine (ISSN 2077-0383). This special issue belongs to the section "General Surgery".

Deadline for manuscript submissions: closed (30 October 2023) | Viewed by 11391

Special Issue Editors

Prof. Dr. Hirofumi Noguchi

E-Mail Website
Guest Editor
Department of Regenerative Medicine, Graduate School of Medicine, University of the Ryukyus, Nishihara 903-0215, Okinawa, Japan
Interests: pancreatic islet transplantation; islet regeneration; stem cell biology
Special Issues, Collections and Topics in MDPI journals
Prof. Dr. Takashi Kenmochi

E-Mail Website
Guest Editor
Department of Organ Transplant Surgery, School of Medicine, Fujita Health University, Toyoake, Japan
Interests: pancreas transplantation; kidney transplantation; pancreatic islet transplantation
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

This Special Issue is a collection of selected papers from the 48th Japanese Society for Organ Preservation and Biology (JSOPB) (http://jognbio.umin.jp/). The Journal of Clinical Medicine (JCM) is providing an opportunity to publish selected data presented at the annual meeting.

The JSOPB was founded in 1974 with the aim of studying organ preservation and rapidly developed during the 1990s following the participation of researchers in various fields of medicine, pharmacology, engineering, veterinary medicine, and basic science. Currently, the JSOPB has more than 700 members and operates under the direction of its president, Prof. Mamoru Kusaka.

The most outstanding presentations made at the 48th annual meeting of the JSOPB—held on 4–5 November 2022, in Hiroshima, Japan under the supervision of Prof. Hideki Ohdan (Department of Gastroenterological and Transplant Surgery, Graduate School of Biomedical and Health Sciences, Hiroshima University. Hiroshima, Japan)—were selected and given the opportunity to be published in this Special Issue of JCM.

One of the vital objectives of the annual meeting of the JSOPB is to exchange new research outcomes and create new therapeutic concepts. Therefore, the aim of the present Special Issue as follows:

  • Molecular and cellular biology of organ preservation and transplantation;
  • Biology of pharmacology;
  • Organ/tissue engineering;
  • Stem cell therapy;
  • Stem cell biology.

Prof. Dr. Hirofumi Noguchi
Prof. Dr. Takashi Kenmochi
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Journal of Clinical Medicine is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • organ preservation
  • transplantation
  • pharmacology
  • engineering
  • molecular biology
  • cellular biology
  • stem cell therapy

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Related Special Issue

Published Papers (6 papers)

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12 pages, 3133 KiB  
Article
Beneficial Effects of Combined Use of Extracorporeal Membrane Oxygenation and Hypothermic Machine Perfusion in Porcine Donors after Cardiac Death for Liver Transplantation
by Hiroyoshi Iwata, Hiromichi Obara, Tetsuya Nakajo, Hiroki Kaneko, Yuga Okazawa, Nur Khatijah Mohd Zin, Hiroki Bochimoto, Makito Ohashi, Yoko Kawada, Mizuho Ohara, Hideki Yokoo and Naoto Matsuno
J. Clin. Med. 2023, 12(18), 6031; https://doi.org/10.3390/jcm12186031 - 18 Sep 2023
Cited by 1 | Viewed by 1502
Abstract
Grafts from donors after cardiac death (DCD) have greatly contributed to expanding the donor organ pool. This study aimed to determine the benefits of subnormothermic extracorporeal membrane oxygenation (ECMO) and hypothermic machine perfusion (HMP) in a porcine model of DCD liver. Female domestic [...] Read more.
Grafts from donors after cardiac death (DCD) have greatly contributed to expanding the donor organ pool. This study aimed to determine the benefits of subnormothermic extracorporeal membrane oxygenation (ECMO) and hypothermic machine perfusion (HMP) in a porcine model of DCD liver. Female domestic crossbred Large Yorkshire and Landrace pigs weighing approximately 20 kg were used. The abdominal aorta and inferior vena cava were cannulated and connected to an ECMO circuit for in situ perfusion of the abdominal organs at 22 °C for 60 min, 45 min after cardiac death. The pigs were divided into the cold storage (CS) group (n = 3), where liver grafts were preserved at 4 °C, and the HMP group (n = 3), where liver grafts were preserved by HMP at 8–10 °C. After 4 h of preservation, liver function was evaluated using an isolated liver reperfusion model for 2 h. Although the difference was insignificant, the liver effluent enzyme levels in the HMP group were lower than those in the CS group. Furthermore, morphological findings showed fewer injured hepatocytes in the HMP group than in the CS group. The combined use of in situ subnormothermic ECMO and HMP was beneficial for the functional improvement of DCD liver grafts. Full article
(This article belongs to the Special Issue JSOPB 2022–2023 Congress Special Issue – Update on Organ Preservation and Transplantation)
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10 pages, 2648 KiB  
Article
An Examination of Packing Methods for Grafts to Prevent Freezing Injury during Transportation for Liver Transplantation
by Kei Kurihara, Taihei Ito, Naohiro Aida, Takashi Kenmochi, Mamoru Kusaka and Hiroto Egawa
J. Clin. Med. 2023, 12(14), 4703; https://doi.org/10.3390/jcm12144703 - 15 Jul 2023
Cited by 1 | Viewed by 1302
Abstract
Background: University of Wisconsin solution (UW) may freeze at temperatures below −0.7 °C, damaging the graft. The present study assessed the effectiveness of the liver graft package protocol, which recommends filling a package with sufficient liquid to prevent grafts from sustaining freezing injury. [...] Read more.
Background: University of Wisconsin solution (UW) may freeze at temperatures below −0.7 °C, damaging the graft. The present study assessed the effectiveness of the liver graft package protocol, which recommends filling a package with sufficient liquid to prevent grafts from sustaining freezing injury. Methods: We filled ice cubes at two temperatures (−80 and −20 °C) around packages and performed a comparative study with four groups based on the temperature and filling of the second layer with lactated Ringer’s solution (LR) (A: −80 °C, LR−; B: −80 °C, LR+; C: −20 °C, LR−; D: −20 °C, LR+). The bovine liver was used as a graft and preserved for 6 h in the first isolation bag filled with UW. Results: While temperatures dropped below −0.7 °C at some points for 6 h in groups A, B, C, they never dropped to −0.7 °C in group D. The macroscopic findings in groups A, B, C showed freezing of the UW and grafts, but no such results in group D. A pathological study including electron microscopy showed freezing injury in groups A, B, and C but no significant changes in group D. Conclusions: The graft package protocol prevents freezing of the UW and liver grafts. Full article
(This article belongs to the Special Issue JSOPB 2022–2023 Congress Special Issue – Update on Organ Preservation and Transplantation)
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14 pages, 3801 KiB  
Article
Exploration of Optimal pH in Hypothermic Machine Perfusion for Rat Liver Grafts Retrieved after Circulatory Death
by Sodai Sakamoto, Hiroki Bochimoto, Kengo Shibata, Nur Khatijah Mohd Zin, Moto Fukai, Kosei Nakamura, Takahisa Ishikawa, Masato Fujiyoshi, Tsuyoshi Shimamura and Akinobu Taketomi
J. Clin. Med. 2023, 12(11), 3845; https://doi.org/10.3390/jcm12113845 - 4 Jun 2023
Cited by 3 | Viewed by 2051
Abstract
Ex vivo hypothermic machine perfusion (HMP) is a strategy for controlling ischemia-reperfusion injury in donation after circulatory death (DCD) liver transplantation. The pH of blood increases with a decrease in temperature and water dissociation, leading to a decrease in [H+]. This [...] Read more.
Ex vivo hypothermic machine perfusion (HMP) is a strategy for controlling ischemia-reperfusion injury in donation after circulatory death (DCD) liver transplantation. The pH of blood increases with a decrease in temperature and water dissociation, leading to a decrease in [H+]. This study aimed to verify the optimal pH of HMP for DCD livers. Rat livers were retrieved 30 min post-cardiac arrest and subjected to 3-h cold storage (CS) in UW solution (CS group) or HMP with UW-gluconate solution (machine perfusion [MP] group) of pH 7.4 (original), 7.6, 7.8, and 8.0 (MP-pH 7.6, 7.8, 8.0 groups, respectively) at 7–10 °C. The livers were subjected to normothermic perfusion to simulate reperfusion after HMP. All HMP groups showed greater graft protection compared to the CS group due to the lower levels of liver enzymes in the former. The MP-pH 7.8 group showed significant protection, evidenced by bile production, diminished tissue injury, and reduced flavin mononucleotide leakage, and further analysis by scanning electron microscopy revealed a well-preserved structure of the mitochondrial cristae. Therefore, the optimum pH of 7.8 enhanced the protective effect of HMP by preserving the structure and function of the mitochondria, leading to reduced reperfusion injury in the DCD liver. Full article
(This article belongs to the Special Issue JSOPB 2022–2023 Congress Special Issue – Update on Organ Preservation and Transplantation)
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15 pages, 4618 KiB  
Article
Warm Ischemia Induces Spatiotemporal Changes in Lysophosphatidylinositol That Affect Post-Reperfusion Injury in Normal and Steatotic Rat Livers
by Kengo Shibata, Takahiro Hayasaka, Sodai Sakamoto, Satsuki Hashimoto, Norio Kawamura, Masato Fujiyoshi, Taichi Kimura, Tsuyoshi Shimamura, Moto Fukai and Akinobu Taketomi
J. Clin. Med. 2023, 12(9), 3163; https://doi.org/10.3390/jcm12093163 - 27 Apr 2023
Cited by 1 | Viewed by 1836
Abstract
Warm ischemia-reperfusion injury is a prognostic factor for hepatectomy and liver transplantation. However, its underlying molecular mechanisms are unknown. This study aimed to elucidate these mechanisms and identify the predictive markers of post-reperfusion injury. Rats with normal livers were subjected to 70% hepatic [...] Read more.
Warm ischemia-reperfusion injury is a prognostic factor for hepatectomy and liver transplantation. However, its underlying molecular mechanisms are unknown. This study aimed to elucidate these mechanisms and identify the predictive markers of post-reperfusion injury. Rats with normal livers were subjected to 70% hepatic warm ischemia for 15, 30, or 90 min, while those with steatotic livers were subjected to 70% hepatic warm ischemia for only 30 min. The liver and blood were sampled at the end of ischemia and 1, 6, and 24 h after reperfusion. The serum alanine aminotransferase (ALT) activity, Suzuki injury scores, and lipid peroxidation (LPO) products were evaluated. The ALT activity and Suzuki scores increased with ischemic duration and peaked at 1 and 6 h after reperfusion, respectively. Steatotic livers subjected to 30 min ischemia and normal livers subjected to 90 min ischemia showed comparable injury. A similar trend was observed for LPO products. Imaging mass spectrometry of normal livers revealed an increase in lysophosphatidylinositol (LPI (18:0)) and a concomitant decrease in phosphatidylinositol (PI (18:0/20:4)) in Zone 1 (central venous region) with increasing ischemic duration; they returned to their basal values after reperfusion. Similar changes were observed in steatotic livers. Hepatic warm ischemia time-dependent acceleration of PI (18:0/20:4) to LPI (18:0) conversion occurs initially in Zone 1 and is more pronounced in fatty livers. Thus, the LPI (18:0)/PI (18:0/20:4) ratio is a potential predictor of post-reperfusion injury. Full article
(This article belongs to the Special Issue JSOPB 2022–2023 Congress Special Issue – Update on Organ Preservation and Transplantation)
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10 pages, 2973 KiB  
Brief Report
Pancreatic Islet Transplantation into the Submandibular Gland: Our Experimental Experience and a Review of the Relevant Literature
by Ibrahim Fathi, Akiko Inagaki, Takehiro Imura, Tarek Koraitim and Masafumi Goto
J. Clin. Med. 2023, 12(11), 3735; https://doi.org/10.3390/jcm12113735 - 29 May 2023
Cited by 3 | Viewed by 1586
Abstract
Pancreatic islet transplantation is a promising therapy for type 1 diabetes. Islet transplantation is clinically performed through intra-portal infusion, which is associated with several drawbacks, including poor engraftment. The histological resemblance between the submandibular gland and the pancreas renders it an attractive alternative [...] Read more.
Pancreatic islet transplantation is a promising therapy for type 1 diabetes. Islet transplantation is clinically performed through intra-portal infusion, which is associated with several drawbacks, including poor engraftment. The histological resemblance between the submandibular gland and the pancreas renders it an attractive alternative site for islet transplantation. In this study, we refined the technique of islet transplantation into the submandibular gland to achieve good morphological features. Then, we transplanted 2600 islet equivalents into the submandibular glands of diabetic Lewis rats. Intra-portal islet transplantation was performed in diabetic rats as a control. Blood glucose levels were followed for 31 days, and an intravenous glucose tolerance test was performed. Immunohistochemistry was used to demonstrate the morphology of transplanted islets. Follow-up after transplantation showed that diabetes was cured in 2/12 rats in the submandibular group in comparison to 4/6 in the control group. The intravenous glucose tolerance test results of the submandibular and intra-portal groups were comparable. Immunohistochemistry showed large islet masses in the submandibular gland in all examined specimens with positive insulin staining. Our results show that submandibular gland tissue can support the islet function and engraftment but with considerable variability. Good morphological features were achieved using our refined technique. However, islet transplantation into rat submandibular glands did not demonstrate a clear advantage over conventional intra-portal transplantation. Full article
(This article belongs to the Special Issue JSOPB 2022–2023 Congress Special Issue – Update on Organ Preservation and Transplantation)
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7 pages, 5717 KiB  
Brief Report
Cryopreservation of Fetal Porcine Kidneys for Xenogeneic Regenerative Medicine
by Kenji Matsui, Yoshitaka Kinoshita, Yuka Inage, Naoto Matsumoto, Keita Morimoto, Yatsumu Saito, Tsuyoshi Takamura, Hitomi Matsunari, Shuichiro Yamanaka, Hiroshi Nagashima, Eiji Kobayashi and Takashi Yokoo
J. Clin. Med. 2023, 12(6), 2293; https://doi.org/10.3390/jcm12062293 - 15 Mar 2023
Cited by 5 | Viewed by 2108
Abstract
Kidney xenotransplantation has been attracting attention as a treatment option for end-stage renal disease. Fetal porcine kidneys are particularly promising grafts because they can reduce rejection through vascularization from host vessels. We are proposing xenogeneic regenerative medicine using fetal porcine kidneys injected with [...] Read more.
Kidney xenotransplantation has been attracting attention as a treatment option for end-stage renal disease. Fetal porcine kidneys are particularly promising grafts because they can reduce rejection through vascularization from host vessels. We are proposing xenogeneic regenerative medicine using fetal porcine kidneys injected with human nephron progenitor cells. For clinical application, it is desirable to establish reliable methods for the preservation and quality assessment of grafts. We evaluated the differentiation potency of vitrified porcine fetal kidneys compared with nonfrozen kidneys, using an in vivo differentiation model. Fetal porcine kidneys connected to the bladder were frozen via vitrification and stored in liquid nitrogen. Several days later, they were thawed and transplanted under the retroperitoneum of immunocompromised mice. After 14 days, the frozen kidneys grew and differentiated into mature nephrons, and the findings were comparable to those of nonfrozen kidneys. In conclusion, we demonstrated that the differentiation potency of vitrified fetal porcine kidneys could be evaluated using this model, thereby providing a practical protocol to assess the quality of individual lots. Full article
(This article belongs to the Special Issue JSOPB 2022–2023 Congress Special Issue – Update on Organ Preservation and Transplantation)
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