E-Mail Alert

Add your e-mail address to receive forthcoming issues of this journal:

Journal Browser

Journal Browser

Special Issue "Heavy Metals Accumulation, Toxicity and Detoxification in Plants"

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Plant Sciences".

Deadline for manuscript submissions: 31 October 2019.

Special Issue Editors

Guest Editor
Prof. Dr. Luigi De Bellis

Department of Biological and Environmental Sciences and Technologies, Università del Salento, Centro Ecotekne, via Provinciale Lecce Monteroni, 73100 Lecce, Italy
Website | E-Mail
Phone: +39-0832-298651
Fax: +39-0832-298626
Interests: plant physiology; genomics; biotechnology; pathology; fruit quality
Guest Editor
Dr. Alessio Aprile

Department of Biological and Environmental Sciences and Technologies, Salento University, Lecce, Italy;
Centro Ecotekne via Provinciale Lecce Monteroni, 73100 Lecce, Italy
E-Mail
Interests: molecular responses f plants to heavy metals, drought and heat; crop physiology of durum wheat and other cereals

Special Issue Information

Dear Colleagues,

In recent years, heavy metals have been widely used in agricultural, chemical, domestic, and technological applications causing environmental and soil contaminations. Heavy metals enter the plant system through soil or via the atmosphere and can accumulate, affecting physiological processes; plant growth; yield; and human health if heavy metals are stored in edible tissues. Understanding the regulation mechanisms of plant heavy metals accumulation and partitioning is important to improve the safety of the food chain.

This Special Issue aims to explore three main issues:

(1) The accumulation and partitioning of heavy metals in crops and wild plants;

(2) The toxicity and molecular behaviours of cells, tissues, and their effects on physiology and plant growth;

(3) Detoxification strategies, plant tolerance, and phytoremediation.

Prof. Dr. Luigi De Bellis
Dr. Alessio Aprile
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1800 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Heavy metals
  • Plant physiological responses to heavy metals
  • Phytoremediation
  • Heavy metal accumulation in plants
  • Toxicity
  • Tolerance
  • Detoxification

Published Papers (6 papers)

View options order results:
result details:
Displaying articles 1-6
Export citation of selected articles as:

Research

Jump to: Review

Open AccessArticle
Ectopic Expression of Poplar ABC Transporter PtoABCG36 Confers Cd Tolerance in Arabidopsis thaliana
Int. J. Mol. Sci. 2019, 20(13), 3293; https://doi.org/10.3390/ijms20133293
Received: 17 May 2019 / Revised: 30 June 2019 / Accepted: 1 July 2019 / Published: 4 July 2019
PDF Full-text (2733 KB) | HTML Full-text | XML Full-text | Supplementary Files
Abstract
Cadmium (Cd) is one of the most toxic heavy metals for plant growth in soil. ATP-binding cassette (ABC) transporters play important roles in biotic and abiotic stresses. However, few ABC transporters have been characterized in poplar. In this study, we isolated an ABC [...] Read more.
Cadmium (Cd) is one of the most toxic heavy metals for plant growth in soil. ATP-binding cassette (ABC) transporters play important roles in biotic and abiotic stresses. However, few ABC transporters have been characterized in poplar. In this study, we isolated an ABC transporter gene PtoABCG36 from Populus tomentosa. The PtoABCG36 transcript can be detected in leaves, stems and roots, and the expression in the root was 3.8 and 2 times that in stems and leaves, respectively. The PtoABCG36 expression was induced and peaked at 12 h after exposure to Cd stress. Transient expression of PtoABCG36 in tobacco showed that PtoABCG36 is localized at the plasma membrane. When overexpressed in yeast and Arabidopsis, PtoABCG36 could decrease Cd accumulation and confer higher Cd tolerance in transgenic lines than in wild-type (WT) lines. Net Cd2+ efflux measurements showed a decreasing Cd uptake in transgenic Arabidopsis roots than WT. These results demonstrated that PtoABCG36 functions as a cadmium extrusion pump participating in enhancing tolerance to Cd through decreasing Cd content in plants, which provides a promising way for making heavy metal tolerant poplar by manipulating ABC transporters in cadmium polluted areas. Full article
(This article belongs to the Special Issue Heavy Metals Accumulation, Toxicity and Detoxification in Plants)
Figures

Figure 1

Open AccessArticle
Comparative Transcriptomic Studies on a Cadmium Hyperaccumulator Viola baoshanensis and Its Non-Tolerant Counterpart V. inconspicua
Int. J. Mol. Sci. 2019, 20(8), 1906; https://doi.org/10.3390/ijms20081906
Received: 3 March 2019 / Revised: 14 April 2019 / Accepted: 16 April 2019 / Published: 17 April 2019
PDF Full-text (1934 KB) | HTML Full-text | XML Full-text | Supplementary Files
Abstract
Many Viola plants growing in mining areas exhibit high levels of cadmium (Cd) tolerance and accumulation, and thus are ideal organisms for comparative studies on molecular mechanisms of Cd hyperaccumulation. However, transcriptomic studies of hyperaccumulative plants in Violaceae are rare. Viola baoshanensis is [...] Read more.
Many Viola plants growing in mining areas exhibit high levels of cadmium (Cd) tolerance and accumulation, and thus are ideal organisms for comparative studies on molecular mechanisms of Cd hyperaccumulation. However, transcriptomic studies of hyperaccumulative plants in Violaceae are rare. Viola baoshanensis is an amazing Cd hyperaccumulator in metalliferous areas of China, whereas its relative V. inconspicua is a non-tolerant accumulator that resides at non-metalliferous sites. Here, comparative studies by transcriptome sequencing were performed to investigate the key pathways that are potentially responsible for the differential levels of Cd tolerance between these two Viola species. A cascade of genes involved in the ubiquitin proteosome system (UPS) pathway were observed to have constitutively higher transcription levels and more activation in response to Cd exposure in V. baoshanensis, implying that the enhanced degradation of misfolded proteins may lead to high resistance against Cd in this hyperaccumulator. Many genes related to sucrose metabolism, especially those involved in callose and trehalose biosynthesis, are among the most differentially expressed genes between the two Viola species, suggesting a crucial role of sucrose metabolism not only in cell wall modification through carbon supply but also in the antioxidant system as signaling molecules or antioxidants. A comparison among transcriptional patterns of some known transporters revealed that several tonoplast transporters are up-regulated in V. baoshanensis under Cd stress, suggesting more efficient compartmentalization of Cd in the vacuoles. Taken together, our findings provide valuable insight into Cd hypertolerance in V. baoshanensis, and the corresponding molecular mechanisms will be useful for future genetic engineering in phytoremediation. Full article
(This article belongs to the Special Issue Heavy Metals Accumulation, Toxicity and Detoxification in Plants)
Figures

Figure 1

Open AccessArticle
Exogenous Glycinebetaine Reduces Cadmium Uptake and Mitigates Cadmium Toxicity in Two Tobacco Genotypes Differing in Cadmium Tolerance
Int. J. Mol. Sci. 2019, 20(7), 1612; https://doi.org/10.3390/ijms20071612
Received: 20 February 2019 / Revised: 23 March 2019 / Accepted: 29 March 2019 / Published: 31 March 2019
PDF Full-text (6555 KB) | HTML Full-text | XML Full-text | Supplementary Files
Abstract
Greenhouse hydroponic experiments were conducted using Cd-sensitive (cv. Guiyan1) and Cd-tolerant (cv. Yunyan2) tobacco cultivars to study the ameliorative effects of exogenous glycinebetaine (GB) upon 5 μM Cd stress. The foliar spray of GB markedly reduced Cd concentrations in plants and [...] Read more.
Greenhouse hydroponic experiments were conducted using Cd-sensitive (cv. Guiyan1) and Cd-tolerant (cv. Yunyan2) tobacco cultivars to study the ameliorative effects of exogenous glycinebetaine (GB) upon 5 μM Cd stress. The foliar spray of GB markedly reduced Cd concentrations in plants and alleviated Cd-induced soil plant analysis development (SPAD) value, plant height and root length inhibition, with the mitigation effect being more obvious in Yunyan2. External GB markedly reduced Cd-induced malondialdehyde (MDA) accumulation, induced stomatal closure, ameliorated Cd-induced damages on leaf/root ultrastructure, and increased the chlorophyll content and fluorescence parameters of Fo, Fm, and Fv/Fm in both cultivars and Pn in Yunyan2. Exogenous GB counteracted Cd-induced alterations of certain antioxidant enzymes and nutrients uptake, e.g., the depressed Cd-induced increase of superoxide dismutase (SOD) and peroxidase (POD) activities, but significantly elevated the depressed catalase (CAT) and ascorbate peroxidase (APX) activities. The results indicate that alleviated Cd toxicity by GB application is related to the reduced Cd uptake and MDA accumulation, balanced nutrients and antioxidant enzyme activities, improved PSII, and integrated ultrastructure in tobacco plants. Full article
(This article belongs to the Special Issue Heavy Metals Accumulation, Toxicity and Detoxification in Plants)
Figures

Figure 1

Open AccessArticle
Transcriptome Analysis Reveals Cotton (Gossypium hirsutum) Genes That Are Differentially Expressed in Cadmium Stress Tolerance
Int. J. Mol. Sci. 2019, 20(6), 1479; https://doi.org/10.3390/ijms20061479
Received: 29 January 2019 / Revised: 18 March 2019 / Accepted: 19 March 2019 / Published: 24 March 2019
PDF Full-text (6527 KB) | HTML Full-text | XML Full-text | Supplementary Files
Abstract
High concentrations of heavy metals in the soil should be removed for environmental safety. Cadmium (Cd) is a heavy metal that pollutes the soil when its concentration exceeds 3.4 mg/kg. Although the potential use of cotton to remediate heavy Cd-polluted soils is known, [...] Read more.
High concentrations of heavy metals in the soil should be removed for environmental safety. Cadmium (Cd) is a heavy metal that pollutes the soil when its concentration exceeds 3.4 mg/kg. Although the potential use of cotton to remediate heavy Cd-polluted soils is known, little is understood about the molecular mechanisms of Cd tolerance. In this study, transcriptome analysis was used to identify Cd tolerance genes and their potential mechanisms in cotton. We exposed cotton plants to excess Cd and identified 4627 differentially expressed genes (DEGs) in the root, 3022 DEGs in the stem and 3854 DEGs in the leaves through RNA-Seq analysis. Among these genes were heavy metal transporter coding genes (ABC, CDF, HMA, etc.), annexin genes and heat shock genes (HSP), amongst others. Gene ontology (GO) analysis showed that the DEGs were mainly involved in the oxidation–reduction process and metal ion binding. The DEGs were mainly enriched in two pathways, the influenza A and pyruvate pathway. GhHMAD5, a protein containing a heavy-metal binding domain, was identified in the pathway to transport or to detoxify heavy metal ions. We constructed a GhHMAD5 overexpression system in Arabidopsis thaliana that showed longer roots compared to control plants. GhHMAD5-silenced cotton plants showed more sensitivity to Cd stress. The results indicate that GhHMAD5 is involved in Cd tolerance, which gives a preliminary understanding of the Cd tolerance mechanism in upland cotton. Overall, this study provides valuable information for the use of cotton to remediate soils polluted with Cd and potentially other heavy metals. Full article
(This article belongs to the Special Issue Heavy Metals Accumulation, Toxicity and Detoxification in Plants)
Figures

Figure 1

Review

Jump to: Research

Open AccessReview
Advances in the Uptake and Transport Mechanisms and QTLs Mapping of Cadmium in Rice
Int. J. Mol. Sci. 2019, 20(14), 3417; https://doi.org/10.3390/ijms20143417
Received: 28 June 2019 / Revised: 10 July 2019 / Accepted: 11 July 2019 / Published: 11 July 2019
PDF Full-text (1606 KB) | HTML Full-text | XML Full-text
Abstract
Cadmium (Cd), as a heavy metal, presents substantial biological toxicity and has harmful effects on human health. To lower the ingress levels of human Cd, it is necessary for Cd content in food crops to be reduced, which is of considerable significance for [...] Read more.
Cadmium (Cd), as a heavy metal, presents substantial biological toxicity and has harmful effects on human health. To lower the ingress levels of human Cd, it is necessary for Cd content in food crops to be reduced, which is of considerable significance for ensuring food safety. This review will summarize the genetic traits of Cd accumulation in rice and examine the mechanism of Cd uptake and translocation in rice. The status of genes related to Cd stress and Cd accumulation in rice in recent years will be summarized, and the genes related to Cd accumulation in rice will be classified according to their functions. In addition, an overview of quantitative trait loci (QTLs) mapping populations in rice will be introduced, aiming to provide a theoretical reference for the breeding of rice varieties with low Cd accumulation. Finally, existing problems and prospects will be put forward. Full article
(This article belongs to the Special Issue Heavy Metals Accumulation, Toxicity and Detoxification in Plants)
Figures

Figure 1

Open AccessReview
Heavy Metal Pollutions: State of the Art and Innovation in Phytoremediation
Int. J. Mol. Sci. 2019, 20(14), 3412; https://doi.org/10.3390/ijms20143412
Received: 20 June 2019 / Revised: 9 July 2019 / Accepted: 10 July 2019 / Published: 11 July 2019
PDF Full-text (1553 KB) | HTML Full-text | XML Full-text
Abstract
Mineral nutrition of plants greatly depends on both environmental conditions, particularly of soils, and the genetic background of the plant itself. Being sessile, plants adopted a range of strategies for sensing and responding to nutrient availability to optimize development and growth, as well [...] Read more.
Mineral nutrition of plants greatly depends on both environmental conditions, particularly of soils, and the genetic background of the plant itself. Being sessile, plants adopted a range of strategies for sensing and responding to nutrient availability to optimize development and growth, as well as to protect their metabolisms from heavy metal toxicity. Such mechanisms, together with the soil environment, meaning the soil microorganisms and their interaction with plant roots, have been extensively studied with the goal of exploiting them to reclaim polluted lands; this approach, defined phytoremediation, will be the subject of this review. The main aspects and innovations in this field are considered, in particular with respect to the selection of efficient plant genotypes, the application of improved cultural strategies, and the symbiotic interaction with soil microorganisms, to manage heavy metal polluted soils. Full article
(This article belongs to the Special Issue Heavy Metals Accumulation, Toxicity and Detoxification in Plants)
Figures

Figure 1

Planned Papers

The below list represents only planned manuscripts. Some of these manuscripts have not been received by the Editorial Office yet. Papers submitted to MDPI journals are subject to peer-review.

Title: Advances in Cadmium Uptake and Transport Mechanisms and QTLs Mapping Related to Cadmium Stress and Cadmium Accumulation in Rice
Author: Jingguang Chen1,2#, Lijun Meng1#, Wenli Zou1, Xiaorong Fan2, Guohua Xu2, Guoyou Ye1*
Affiliation: 1CAAS-IRRI Joint Laboratory for Genomics-Assisted Germplasm Enhancement, Agricultural Genomics Institute in Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China. 2State Key Laboratory of Crop Genetics and Germplasm Enhancement, MOA Key Laboratory of Plant Nutrition and Fertilization in Low-Middle Reaches of the Yangtze River, Nanjing Agricultural University, Nanjing 210095, China.
Abstract: Cadmium (Cd) is a kind of heavy metal that has extremely strong biological toxicity and is extremely harmful to human health. In order to reduce human Cd uptake, Cd content in food crops must be reduced, which is of great significance to ensure food safety. This review will summarize the genetic traits of Cd accumulation in rice and examine the mechanism of Cd uptake and translocation in rice. The status of QTLs related to Cd stress and Cd accumulation in rice in recent years was summarized, and the genes related to Cd accumulation in rice were classified according to their functions. In addition, the overview of QTLs mapping populations in rice was introduced, aiming to provide theoretical reference for the breeding of rice varieties with low Cd accumulation. Finally, its existing problems and prospects were put forward.

 

Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top