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Special Issue "Cell-Specificity in Plants"
Deadline for manuscript submissions: 29 November 2019.
Institute for Sustainable Plant Protection (IPSP)-CNR, Torino, Italy
Interests: cell wall; genomics and fungal genomics of symbiotic fungi; mycorrhizal fungi; plant-microbe interactions; abiotic stresses
Special Issues and Collections in MDPI journals
Special Issue in Agriculture: Plant-Microbe Interactions
Dr. Valentina Fiorilli
Department of Life Sciences and Systems Biology, University of Torino, Turin, Italy
Interests: molecular and cellular aspects of plant–microbe interactions, mainly during arbuscular mycorrhizal symbiosis; plant response to biotic stress; role of phytohormones in plant growth and in response to microbes; plant biology; plant nutrition; plant–microbe interactions; arbuscular mycorrhizal symbiosis; mycorrhizal-induced resistance and susceptibility
Plant growth and interactions take place in a complex cellular environment. Organs are complex structures composed of different tissues characterized by distinct cell types. Each cell type has specific functions that are driven by its own unique transcriptome, proteome, and metabolome. Global cell-profiling studies can provide information on several transcripts unique to specialized cells or, at least, localized to a few cell types. The examination of functional processes at the molecular level suffers from the use of whole organs, and cell type-specific differences might be masked by this approach. Even if transcripts corresponding to genes of interest are very abundant in one or few cell types, their amount may be diluted within the whole RNA pool of a tissue. Studies aimed to describe protein and metabolite profiles are also complicated by the presence of multiple cell types. The application of different approaches, such as plant transformation, in situ hybridization, and, more recently, laser microdissection (LMD), has allowed the identification of the localized expression of specific genes in specific cell populations. This is particularly important for genes predominantly expressed in cell types that are present in a small number in the analyzed tissue.
Dr. Raffaella Maria Balestrini
Dr. Valentina Fiorilli
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- Cell specificity
- Laser microdissection
- In situ hybridization
- Reporter gene
- Plant interactions