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Special Issue "Applications of Advanced Imaging and Analytical Microscopic Techniques on Molecular and Cell Biology"

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Biology".

Deadline for manuscript submissions: 31 May 2021.

Special Issue Editor

Dr. Chan-Gi Pack
Website
Guest Editor
1. Department of Convergence Medicine, University of Ulsan College of Medicine, Seoul 05505, Korea<br/>2. Convergence Medicine Research Center, Asan Medical Center, Seoul 05505, Korea
Interests: nanoparticle/drug-cell interaction; cellular drug delivery; protein dynamics and interaction; cellular biophysics; fluorescence correlation spectroscopy; optical diffraction tomography; immuno-gold EM; CLEM

Special Issue Information

Dear Colleagues,

Various imaging techniques have long provided researchers with specific and quantitative information about molecules and cells through a wide variety of imaging modalities, from conventional microscopic and nonmicroscopic techniques to advanced and super-resolution level techniques. Today, the development and improvement of such imaging techniques allow for the establishment of next-generation biological and biomedical techniques. Moreover, the synthesis of established imaging techniques combined with analytical techniques provides new insight into various fields of life science. This Special Issue aims to serve as a guideline of various imaging and analytical methods of cells and provide a wide range of their applications.

Research on applications is expected to target specific biological and biophysical questions, which include quantification and functional analyses of the molecular and cellular states in fixed and live cells. Moreover, the content of the Special issue covers reports uncovering the molecular mechanisms underpinning biological processes encompassing various disciplines of biology and medicine.

We would like to welcome authors to submit research and review articles for the Special Issue “Applications of Advanced Imaging and Analytical Microscopic Techniques on Molecular and Cell Biology”.

Dr. Chan-Gi Pack
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • super-resolution microscopy
  • multiphoton microscopy
  • fluorescence correlation methods
  • fluorescence lifetime imaging
  • label-free microscopic imaging
  • electron microscopy
  • single-molecule imaging
  • fluorescent probe
  • cellular organelles
  • molecular function
  • molecular interaction
  • diffusion dynamics
  • disease- and drug-related subcellular phenomena

Published Papers (1 paper)

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Research

Open AccessArticle
Vibrational Spectroscopic Investigation of Blood Plasma and Serum by Drop Coating Deposition for Clinical Application
Int. J. Mol. Sci. 2021, 22(4), 2191; https://doi.org/10.3390/ijms22042191 (registering DOI) - 22 Feb 2021
Abstract
In recent decades, vibrational spectroscopic methods such as Raman and FT-IR spectroscopy are widely applied to investigate plasma and serum samples. These methods are combined with drop coating deposition techniques to pre-concentrate the biomolecules in the dried droplet to improve the detected vibrational [...] Read more.
In recent decades, vibrational spectroscopic methods such as Raman and FT-IR spectroscopy are widely applied to investigate plasma and serum samples. These methods are combined with drop coating deposition techniques to pre-concentrate the biomolecules in the dried droplet to improve the detected vibrational signal. However, most often encountered challenge is the inhomogeneous redistribution of biomolecules due to the coffee-ring effect. In this study, the variation in biomolecule distribution within the dried-sample droplet has been investigated using Raman and FT-IR spectroscopy and fluorescence lifetime imaging method. The plasma-sample from healthy donors were investigated to show the spectral differences between the inner and outer-ring region of the dried-sample droplet. Further, the preferred location of deposition of the most abundant protein albumin in the blood during the drying process of the plasma has been illustrated by using deuterated albumin. Subsequently, two patients with different cardiac-related diseases were investigated exemplarily to illustrate the variation in the pattern of plasma and serum biomolecule distribution during the drying process and its impact on patient-stratification. The study shows that a uniform sampling position of the droplet, both at the inner and the outer ring, is necessary for thorough clinical characterization of the patient’s plasma and serum sample using vibrational spectroscopy. Full article
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