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New Advances in Myocardial Regeneration: Molecular Mechanisms and Promising Approaches

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Biology".

Deadline for manuscript submissions: closed (20 March 2025) | Viewed by 3112

Special Issue Editor


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Guest Editor
First Department of Medicine, Cardiology, Angiology, Hemostaseology and Internal Intensive Care Medicine, University Medical Centre Mannheim, 68167 Mannheim, Germany
Interests: cardiomyocyte function; ischemia; inflammation and metabolic models; arrhythmias; molecular biomarkers; intracellular pathways
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Special Issue Information

Dear Colleagues,

Myocardial infarction (MI) and consequent heart failure remain the leading cause of death worldwide and a major burden on healthcare systems. This Special Issue "New Advances in Myocardial Regeneration" aims to focus on the latest basic and translational research aimed at finding a cure for heart failure due to cardiomyocyte loss and/or dysfunction.

This Special Issue aims to highlight key findings and promising approaches to cardiac remuscularisation, but may also discuss remaining challenges or critical issues regarding internal regenerative processes (resident factors or cells; the direct reprogramming of resident cells) or external approaches (the transplantation of cells; the application of substances).

Both original studies and reviews will be published. These could include topics such as cardiac organoids, modified RNA, single cell omics, 3D bioprinting, exosome delivery, and machine learning approaches. Any type of research dedicated to finding new effective approaches to myocardial regeneration will be of interest.

Dr. Katherine Sattler
Guest Editor

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Keywords

  • myocardial regeneration
  • transplantation of cells
  • proliferation of cardiomyocytes
  • 3D bioprinting
  • single cell omics
  • exosome delivery
  • cardiac organoids
  • direct reprogramming

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Published Papers (1 paper)

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Research

22 pages, 3235 KiB  
Article
Advancing Human iPSC-Derived Cardiomyocyte Hypoxia Resistance for Cardiac Regenerative Therapies through a Systematic Assessment of In Vitro Conditioning
by Caroline A. Snyder, Kiera D. Dwyer and Kareen L. K. Coulombe
Int. J. Mol. Sci. 2024, 25(17), 9627; https://doi.org/10.3390/ijms25179627 - 5 Sep 2024
Cited by 1 | Viewed by 2251
Abstract
Acute myocardial infarction (MI) is a sudden, severe cardiac ischemic event that results in the death of up to one billion cardiomyocytes (CMs) and subsequent decrease in cardiac function. Engineered cardiac tissues (ECTs) are a promising approach to deliver the necessary mass of [...] Read more.
Acute myocardial infarction (MI) is a sudden, severe cardiac ischemic event that results in the death of up to one billion cardiomyocytes (CMs) and subsequent decrease in cardiac function. Engineered cardiac tissues (ECTs) are a promising approach to deliver the necessary mass of CMs to remuscularize the heart. However, the hypoxic environment of the heart post-MI presents a critical challenge for CM engraftment. Here, we present a high-throughput, systematic study targeting several physiological features of human induced pluripotent stem cell-derived CMs (hiPSC-CMs), including metabolism, Wnt signaling, substrate, heat shock, apoptosis, and mitochondrial stabilization, to assess their efficacy in promoting ischemia resistance in hiPSC-CMs. The results of 2D experiments identify hypoxia preconditioning (HPC) and metabolic conditioning as having a significant influence on hiPSC-CM function in normoxia and hypoxia. Within 3D engineered cardiac tissues (ECTs), metabolic conditioning with maturation media (MM), featuring high fatty acid and calcium concentration, results in a 1.5-fold increase in active stress generation as compared to RPMI/B27 control ECTs in normoxic conditions. Yet, this functional improvement is lost after hypoxia treatment. Interestingly, HPC can partially rescue the function of MM-treated ECTs after hypoxia. Our systematic and iterative approach provides a strong foundation for assessing and leveraging in vitro culture conditions to enhance the hypoxia resistance, and thus the successful clinical translation, of hiPSC-CMs in cardiac regenerative therapies. Full article
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