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Selected Papers Associated with 2023 STEMNET MEETING

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A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Biochemistry".

Deadline for manuscript submissions: closed (20 March 2024) | Viewed by 2220

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Special Issue Editors

Dr. Katia Mareschi

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Guest Editor

1. Department of Public Health and Paediatrics, The University of Turin, Piazza Polonia 94, 10126 Torino, Italy
2. Stem Cell Transplantation and Cellular Therapy Laboratory, Paediatric Onco-Haematology Division, Regina Margherita Children’s Hospital, City of Health and Science of Turin, 10126 Torino, Italy
Interests: mesenchymal stem cells; GMP production; cell therapy
Special Issues, Collections and Topics in MDPI journals

Dr. Ivana Ferrero

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Guest Editor

Stem Cell Transplantation and Cellular Therapy Laboratory, Regina Margherita Children’s Hospital, City of Health and Science of Turin, 10126 Turin, Italy
Interests: stem cells; cell therapy

Special Issue Information

Dear Colleagues,

The Second International StemNet Meeting will take place in Brescia (Italy) on October 18-20, 2023, and in this occasion, we would like to share the scientific works of national and international speakers in the field of advanced cell therapies.

StemNet is a federation of the four main associations of stem cell research in Italy (FIRST, GISM, IPLASS, SCRI) that share and synergize experiences to enhance both the quality and the impact of research in this advancing field. The nine sessions of the meeting reflect the long lasting relationship among these associations and aim to improve the exchange of relevant and up-to-date information in basic and translational stem cell research in human and veterinary medicine.

Abstracts accepted for the conference can be published in this Special Issue of International Journal of Molecular Sciences in a conference report (subject to peer review).

Besides papers related directly to the presentations delivered during the conference, we warmly invite all those researchers whose work employs stem cells to submit a manuscript to this special issue.

Dr. Katia Mareschi
Dr. Ivana Ferrero
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

mesenchymal stromal cell
advanced cell therapy
translational medicine
stem cell in veterinary
organoid
secretome

Published Papers (3 papers)

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20 pages, 3762 KiB

Open AccessArticle

Mesenchymal Stromal Cells Derived from Canine Adipose Tissue: Evaluation of the Effect of Different Shipping Vehicles Used for Clinical Administration

by Valentina Andreoli, Priscilla Berni, Virna Conti, Roberto Ramoni, Giuseppina Basini and Stefano Grolli

Int. J. Mol. Sci. 2024, 25(6), 3426; https://doi.org/10.3390/ijms25063426 - 18 Mar 2024

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Abstract

Mesenchymal Stromal Cells (MSCs)-based therapies are rapidly gaining interest in veterinary medicine. Cellular therapy represents a new challenge for practitioners and requires precise coordination between the cell processing laboratory and the veterinary clinic. Cryopreservation is the best method to provide fast, in-time, and long-distance delivery of cells for therapeutic applications. However, potentially toxic cryoprotectants and xenobiotic products make the direct administration of cells impracticable for patients. Alternatively, the cells may be resuspended in a ready-to-use vehicle and shipped to the veterinary clinic. In this study, two nutrient-poor vehicles (physiologic saline and ringer lactate solutions) and two nutrient-rich vehicles (the releasate derived from autologous Platelet Poor Plasma and Platelet Rich Plasma) were tested on adipose tissue-derived canine MSCs (AD-MSCs). AD-MSCs stored for 2, 4, or 24 h in the different media were compared regarding mortality, metabolic activity, and replicative capacity. Furthermore, antioxidant activity and the pattern of expression of genes related to AD-MSCs function were performed following 24 h of storage. The results showed that all the different vehicles preserve cell vitality and replication following short-term storage. In long-term storage, the vehicle and cell density affect cell vitality, proliferation, and gene expression (CCL-2, CXCR-4, and TSG-6). Nutrient-rich vehicles seem better suited to preserve cell functionalities in this contest. Full article

(This article belongs to the Special Issue Selected Papers Associated with 2023 STEMNET MEETING)

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17 pages, 2624 KiB

Open AccessArticle

Stable Housekeeping Genes in Bone Marrow, Adipose Tissue, and Amniotic Membrane-Derived Mesenchymal Stromal Cells for Orthopedic Regenerative Medicine Approaches

by Enrico Ragni, Simona Piccolo, Andrea Papait, Paola De Luca, Michela Taiana, Giulio Grieco, Antonietta Rosa Silini, Ornella Parolini and Laura de Girolamo

Int. J. Mol. Sci. 2024, 25(3), 1461; https://doi.org/10.3390/ijms25031461 - 25 Jan 2024

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Abstract

The therapeutic effect of mesenchymal stromal cells (MSCs) has been described for a variety of disorders, including those affecting musculoskeletal tissues. In this context, the literature reports several data about the regenerative effectiveness of MSCs derived from bone marrow, adipose tissue, and an amniotic membrane (BMSCs, ASCs, and hAMSCs, respectively), either when expanded or when acting as clinical-grade biologic pillars of products used at the point of care. To date, there is no evidence about the superiority of one source over the others from a clinical perspective. Therefore, a reliable characterization of the tissue-specific MSC types is mandatory to identify the most effective treatment, especially when tailored to the target disease. Because molecular characterization is a crucial parameter for cell definition, the need for reliable normalizers as housekeeping genes (HKGs) is essential. In this report, the stability levels of five commonly used HKGs (ACTB, EF1A, GAPDH, RPLP0, and TBP) were sifted into BMSCs, ASCs, and hAMSCs. Adult and fetal/neonatal MSCs showed opposite HKG stability rankings. Moreover, by analyzing MSC types side-by-side, comparison-specific HKGs emerged. The effect of less performant HKG normalization was also demonstrated in genes coding for factors potentially involved in and predicting MSC therapeutic activity for osteoarthritis as a model musculoskeletal disorder, where the choice of the most appropriate normalizer had a higher impact on the donors rather than cell populations when compared side-by-side. In conclusion, this work confirms HKG source-specificity for MSCs and suggests the need for cell-type specific normalizers for cell source or condition-tailored gene expression studies. Full article

(This article belongs to the Special Issue Selected Papers Associated with 2023 STEMNET MEETING)

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14 pages, 995 KiB

Open AccessConference Report

State of the Art and New Trends from the Second International StemNet Meeting

by Ivana Ferrero, Filippo Piccinini, Pasquale Marrazzo, Manuela Monti, Caterina Pipino, Alessia Santa Giovanna Banche Niclot, Camilla Francesca Proto, Enrico Ragni, Ralf Hass, Giulia Maria Stella, Priscilla Berni, Ana Ivanovska and Katia Mareschi

Int. J. Mol. Sci. 2024, 25(4), 2221; https://doi.org/10.3390/ijms25042221 - 13 Feb 2024

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Abstract

The Second International StemNet (Federation of Stem Cell Research Associations) meeting took place on 18–20 October 2023 in Brescia (Italy), with the support of the University of Brescia and the Zooprophylactic Institute of Lombardy and Emilia Romagna. The program of the meeting was articulated in nine sections: (1) Biomedical Communication in Italy: Critical Aspects; (2) StemNet Next Generation Session; (3) Cell-Free Therapies; (4) Tips and Tricks of Research Valorisation; (5) Stem Cells and Cancer; (6) Stem Cells in Veterinary Applications; (7) Stem Cells in Clinical Applications; (8) Organoids and 3D Systems; (9) induced pluripotent stem cells (iPCS) and Gene Therapy. National and International speakers presented their scientific works, inspiring debates and discussions among the attendees. The participation in the meeting was high, especially because of the young researchers who animated all the sessions and the rich poster session. Full article

(This article belongs to the Special Issue Selected Papers Associated with 2023 STEMNET MEETING)

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